首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (2): 251-257.doi: 10.3969/j.issn.1006-7795.2013.02.017

• 临床研究 • 上一篇    下一篇

Toll样受体11~13在肥大细胞上的表达

李艳1,2, 李波3, 姚秀娟1,2, 吕喆2, 安云庆2, 王炜2, 黄克武1, 孙英2,4   

  1. 1. 首都医科大学附属北京朝阳医院呼吸与危重症医学科北京呼吸疾病研究所, 北京 100020;
    2. 首都医科大学基础医学院免疫学系, 北京 100069;
    3. 中国食品药品检定研究院食品化妆品检定所风险评估室, 北京 100050;
    4. Division of Asthma, Allergy & Lung Biology, King’s College London, Medical Research Council & Asthma United Kingdom Centre in Allergic Mechanisms of Asthma, London SE19RT
  • 收稿日期:2012-12-26 出版日期:2013-04-21 发布日期:2013-04-17
  • 通讯作者: 王炜, 黄克武 E-mail:wy_robin@ccmu.edu.cn; kwhuang@hotmail.com
  • 基金资助:

    国家自然科学基金(81102250,81072446),北京市教育委员会科技发展计划面上项目(KM201010025002,KM201110025005),北京市属高等学校人才强教深化计划"中青年骨干人才培养计划"项目(PHR201008382)

Expression of Toll-like receptor 11~13 in mast cells

LI Yan1,2, LI Bo3, YAO Xiujuan1,2, LV Zhe2, AN Yunqing2, WANG Wei2, HUANG Kewu1, SUN Ying2,4   

  1. 1. Division of Pulmonary and Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing Institute of Respiratory Medicine, Beijing 100020, China;
    2. Department of Immunology, School of Basic Medical Science, Capital Medical University, Beijing 100069, China;
    3. Division of Risk Assessment, Institute for Food and Cosmetics Control, National Institutes for Food and Drug Control, Beijing 100050, China;
    4. Division of Asthma, Allergy & Lung Biology, King’s College London, Medical Research Council & Asthma United Kingdom Centre in Allergic Mechanisms of Asthma, London SE19RT, United Kingdom
  • Received:2012-12-26 Online:2013-04-21 Published:2013-04-17
  • Supported by:

    This study was supported by National Natural Science Foundation of China(81102250,81072446), the Science and Technology Project of the Beijing Municipal Education Commission(KM201010025002, KM201110025005), Funding Project for Academic Human Resources Development in Institutions of Higher Learning Under the Jurisdiction of Beijing Municipality(PHR201008382)

摘要:

目的 观察Toll样受体(Toll like receptors,TLRs)11~13在小鼠肥大细胞上的表达。方法 用反转录聚合酶链式反应(reverse transcription polymerase chain reaction,RT-PCR)、流式细胞术和免疫荧光染色方法,在mRNA和蛋白质水平检测TLR11~TLR13在小鼠骨髓来源的肥大细胞(bone marrow-derived mast cell,BMMC)和小鼠肥大细胞系MC/9的表达。结果 BMMC和MC/9细胞在mRNA和蛋白质水平均能表达TLR11~TLR13。结论 发现肥大细胞表达TLR11~TLR13,为进一步研究其生物学功能提供实验依据。

关键词: 肥大细胞, Toll样受体11, Toll样受体12, Toll样受体13

Abstract:

Objective To identify whether murine mast cells express Toll-like receptors 11-13(TLR11-13). Methods Reverse transcription polymerase chain reaction(RT-PCR), flow cytometry and immunofluorescent staining were used to detect the expression of TLR11-13 in murine bone marrow-derived mast cell(BMMC) and mast cell line MC/9 at the levels of mRNA and protein. Results Both BMMC and MC/9 expressed TLR11-13 at both mRNA and protein levels. Conclusion The expression of TLR11-13 by mast cells has provided clues to future investigating biological functions of these TLRs in mast cells.

Key words: mast cells, Toll-like receptor11, Toll-like receptor12, Toll-like receptor13

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