白介素1受体辅助蛋白在儿童和成人低级别胶质瘤中的作用

doi:10.3969/j.issn.1006-7795.2013.02.018

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (2): 258-263.doi: 10.3969/j.issn.1006-7795.2013.02.018

白介素1受体辅助蛋白在儿童和成人低级别胶质瘤中的作用

邢树山¹, 胡万宁¹, 赵喜庆¹, 刘文庆², 宫剑³

1. 河北联合大学附属唐山市人民医院肿瘤科, 唐山 063000;
2. 宁夏医科大学总医院神经外科, 银川 750004;
3. 首都医科大学附属北京天坛医院神经外科, 北京 100050

收稿日期:2012-04-20 出版日期:2013-04-21 发布日期:2013-04-17
通讯作者: 宫剑 E-mail:gongjian88@tom.com
基金资助:
国家自然科学基金(81072074、31271119),北京市教委科技计划重点项目(KZ201110025033),北京市优秀人才基金(2011D003034000004)资助。

Interleukin 1 receptor accessory protein contributes to children and adult low grade gliomas

XING Shushan¹, HU Wanning¹, ZHAO Xiqing¹, LIU Wenqing², GONG Jian³

1. Department of Oncology, Tangshan People’ s Hospital, Hebei United University, Tangshan 063000, Hebei Province, China;
2. Department of Neurosurgery, Ningxia Medical University, Yinchuan 750004, China;
3. Department of Pediatric Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China

Received:2012-04-20 Online:2013-04-21 Published:2013-04-17
Supported by:
This study was supported by National Natural Science Foundation of China(81072074, 31271119), Key Project of Beijing Municipal Education Commission(KZ201110025033) and Beijing Excellent Talent Personal Training Foundation(2011D003034000004).

摘要/Abstract

摘要：

目的探讨白介素1受体辅助蛋白(interleukin 1 receptor accessory protein,IL-1RAP)在成人与儿童低级别胶质瘤中的表达,分析其与预后的相关性。方法应用高通量全基因分析、免疫组织化学法、qRT-PCR和Western blotting方法对20例低级别胶质瘤(成人10例,儿童10例)标本中IL-1RAP的表达进行检测。结果经高通量全基因分析、免疫组织化学、qRT-PCR和Western blotting分析证明IL-1RAP与成人比较,儿童低级别胶质瘤总阳性表达率较低,有统计学意义(100%>30%,Chi-Square=12.439,P<0.05)。结论 IL-1RAP可能在胶质瘤的发生、发展过程之中扮演重要的角色。IL-1RAP可能成为评价低级别胶质瘤预后差异的重要参考指标及潜在的药物靶点。

关键词: 白介素1受体辅助蛋白, 低级别胶质瘤, 成人, 儿童

Abstract:

Objective To analyze the expression of interleukin 1 receptor accessory protein(IL-1RAP) in children and adult low grade gliomas(LGG) and correlation between the level of this markers and low grade gliomas recurrence. Methods The samples of LGG were collected and divided into two groups(adult group and children group).Gene expression profiles in adult group and children group were compared by microarray. The expression of changed gene was indentified by immunohistochemistry(IHC), Western blot and quantitative real-time PCR(qRT-PCR) in 20 cases of LGG(10 children, 10 adult). Results We identified that IL-1RAP was consistently up-regulated in children group LGG with the adult group LGG by cDNA array, and the level of IL-1RAP in children group was significantly higher than that of adult group detected by RT-PCR, Western blot and IHC(Chi-Square=12.439, P<0.05). Conclusion IL-1RAP may be the potential biomarkers to evaluate the inclination of invasion and recurrence in children and adult low grade gliomas. And the dysregulation of IL-1RAP may be critical for malignant astrocytoma formation, and thus a possible novel universal target for therapeutic intervention.

Key words: interleukin 1 receptor accessory protein(IL-1RAP), low grade gliomas, adult, children

中图分类号:

R730.264

邢树山, 胡万宁, 赵喜庆, 刘文庆, 宫剑. 白介素1受体辅助蛋白在儿童和成人低级别胶质瘤中的作用[J]. 首都医科大学学报, 2013, 34(2): 258-263.

XING Shushan, HU Wanning, ZHAO Xiqing, LIU Wenqing, GONG Jian. Interleukin 1 receptor accessory protein contributes to children and adult low grade gliomas[J]. Journal of Capital Medical University, 2013, 34(2): 258-263.

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白介素1受体辅助蛋白在儿童和成人低级别胶质瘤中的作用

Interleukin 1 receptor accessory protein contributes to children and adult low grade gliomas

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