IL-17BR在人血管内皮细胞和哮喘患者气道黏膜组织中的表达

首都医科大学学报 ›› 2010, Vol. 31 ›› Issue (2): 245-250.

IL-17BR在人血管内皮细胞和哮喘患者气道黏膜组织中的表达

范宜强¹, 王炜^1,4, 吕喆¹, 许晴², 孟秋³, 孙英^3*, 安云庆^1,4*

1. 首都医科大学基础医学院免疫学系;2. 首都医科大学基础医学院生殖医学系;3. 伦敦大学国王学院,英国卫生部哮喘研究中心暨英国伦敦大学哮喘、过敏与呼吸科学系;4. 首都医科大学呼吸病学系

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-21 发布日期:2010-04-21
通讯作者: 孙英，安云庆

Expression of IL-17BR in Human Blood Vascular Endothelial Cell and Asthmatic Airways Mucosa

FAN Yi-qiang¹, WANG Wei^1,4, LV Zhe¹, XU Qing², MENG Qiu³, SUN Ying^3*, AN Yun-qing^1,4*

1. Department of Immunology, School of Basic Medical Sciences, Capital Medical University; 2. Reproductive Medical Center, School of Basic Medical Sciences, Capital Medical University; 3. King's College London, MRC & Asthma UK Centre in Allergic Mechanisms of Asthma, Division of Asthma, Allergy & Lung Biology, London UK; 4. Department of Respiratory Disease, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2010-04-21 Published:2010-04-21
Contact: SUN Ying, AN Yun-qing

摘要/Abstract

摘要：

目的检测人脐静脉内皮细胞(human umbilical vein endothelial cells，HUVEC)和哮喘患者支气管黏膜组织白细胞介素-17BR（IL-17BR）（IL-25受体）的表达情况，为深入探讨IL-25是否参与哮喘发生发展和气道重塑提供实验依据。方法采用免疫细胞化学方法检测HUVEC 能否表达IL-17BR；采用RT-PCR和Real-time PCR检测HUVEC接受IL-25（1 μg/L）刺激前后IL-17BR基因表达水平的变化；采用亲和素-生物素复合系统检测哮喘患者支气管活检组织中IL-17BR的表达情况。结果 HUVEC可组成性表达IL-17BR，接受IL-25（1 μg/L）刺激后，IL-17BR（IL-25R）基因表达水平显著升高；哮喘患者支气管活检组织中有大量IL-17BR阳性细胞，而正常对照人群相应活检组织中仅有极少数IL-17BR阳性细胞。结论 HUVEC和支气管黏膜组织可组成性表达IL-17BR，提示人血管内皮细胞和黏膜组织中IL-17BR阳性细胞有可能通过表面IL-17BR与IL-25结合相互作用参与哮喘的发生发展过程。

关键词: 人脐静脉内皮细胞, 白细胞介素-25, 白细胞介素-17BR

Abstract:

Objective To investigate the expression of IL-17BR(IL-25R) from human vascular endothelial cell(HUVEC) and bronchial biopsies of asthmatics compared with controls. Methods Immunocytochemical method was employed to detect the expression of IL-17BR in cultured human umbilical vein endothelial cells(HUVEC). Reverse transcription-polymerase chain reaction (RT-PCR) and real-time quantitative PCR were used to study the diversification of IL-17BR gene expression in HUVEC cultured with or without rhIL-25. Avidin-biotin complex(ABC) method was employed for detecting IL-17BR immunoreactivity in bronchial biopsies. Results Immunocytochemistry showed that HUVEC constitutively expressed IL-17BR. PCR showed that the expression of IL-17BR mRNA was upregulated by its ligand, IL-25 (1 μg/L). The increased number of IL-17BR immunoreactive cells were found in the bronchial biopsies of asthmatics, whereas extremely few cells of the normal controls were positive for IL-17BR. Conclusion IL-17BR is constitutively expressed in HUVEC and airways bronchial mucosa and increases in asthmatic airways. It suggests that the IL-17BR positive cells in human bronchial vascular endothelial cells and airways mucosa may participate in the pathogenesis of asthma through interaction of IL-25 with its receptor.

Key words: human umbilical vein endothelial cells, interleukin-25, interleukin-17BR

中图分类号:

R 392.3， R 562.2⁺5

范宜强;王炜;吕喆;许晴;孟秋;孙英;安云庆;. IL-17BR在人血管内皮细胞和哮喘患者气道黏膜组织中的表达[J]. 首都医科大学学报, 2010, 31(2): 245-250.

FAN Yi-qiang;WANG Wei;LV Zhe;XU Qing;MENG Qiu;SUN Ying;AN Yun-qing;. Expression of IL-17BR in Human Blood Vascular Endothelial Cell and Asthmatic Airways Mucosa[J]. Journal of Capital Medical University, 2010, 31(2): 245-250.