首都医科大学学报 ›› 2010, Vol. 31 ›› Issue (2): 251-254.doi: 10.3969/j.issn.1006-7795.2010.02.022

• 基础研究 • 上一篇    下一篇

中药复方912注射液对MODS动物模型细胞因子的实验研究

任添华1, 张淑文2*   

  1. 1. 首都医科大学附属北京天坛医院急诊科;2. 首都医科大学附属北京友谊医院感染暨急救医学科
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-21 发布日期:2010-04-21
  • 通讯作者: 张淑文

Experimental Research on the Effects of a Traditional Chinese Medicine Complex 912 for Injection on Serum Cytokine Levels in the Multiple Organ Dysfunction Syndrome Animal Model

REN Tian-hua1, ZHANG Shu-wen2*   

  1. 1. Department of Emergency, Beijing Tiantan Hospital, Capital Medical University;2. Department of Infection and Critical Care, Beijing Friendship Hospital, Capital Medical University
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-04-21 Published:2010-04-21
  • Contact: ZHANG Shu-wen

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222

被引次数

6

摘要： 目的 为探讨中药复方912注射液对多器官功能障碍综合征（multiple organ dysfunction syndrome，MODS）动物模型血清细胞因子的影响。方法 选择体质量在20~25 kg的一年期雄性健康小香猪32只，以标准大肠杆菌由右侧颈内静脉缓慢注入，于注入5 h后制成MODS动物模型。将这些MODS动物模型随机分为912注射液治疗组和0.9%氯化钠注射液对照组。2组动物模型分别于治疗前及治疗后1 h、3 h、5 h、7 h检测血清内毒素（endotoxin，EX）、血清细胞因子、肿瘤坏死因子（tumor necrosis factor，TNF）、白细胞介素1（interleukin-1，IL-1）、白细胞介素6（interleukin-6，IL-6）、白细胞介素10（interleukin-10，IL-10）、一氧化氮（nitric oxide，NO）。结果 912注射液治疗组于治疗开始3 h后各血清细胞因子的水平明显低于治疗前水平（P<0.01），也明显低于对照组（P<0.01）。结论 这些结果为912注射液对MODS的进一步临床治疗研究提供了实验基础。

关键词: 912液, 多器官功能障碍综合征, 动物模型, 细胞因子

Abstract: Objective To study the effect of traditional Chinese medicine complex 912 for injection on the serum cytokine level of the multiple organ dysfunction syndrome(MODS) animal model. Methods Thirtytwo oneyearold male healthy piglets with body weight 20~25 kg were used for this study. Standard Escherichia coli(17 million/kg) was injected into right jugular vein slowly. Five hours after the injection all the animals developed MODS, and these MODS model animals were randomly divided into the following two groups: 912 complex treatment group and normal saline control group. Serum endotoxin and cytokines including tumor necrosis factor(TNF), interleukin1(IL1), interleukin6(IL6), interleukin10(IL10), and nitric oxide(NO) were determined at 1 h, 3 h, 5 h and 7 h after treatment. Results The serum cytokines decreased 3 h after treatment with 912 complex injection, and were lower than those of the normal saline control group at the same time points. Conclusion These finding provide the experimental basis for further study on treatment of MODS with traditional Chinese medicine 912 complex injection.

Key words: components 912 injection, multiple organ dysfunction syndrome, animal model, cytokines

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