黄芩苷对脂多糖诱导的人单核细胞THP-1的炎症反应的作用

doi:10.3969/j.issn.1006-7795.2022.06.012

首都医科大学学报 ›› 2022, Vol. 43 ›› Issue (6): 893-898.doi: 10.3969/j.issn.1006-7795.2022.06.012

黄芩苷对脂多糖诱导的人单核细胞THP-1的炎症反应的作用

王平^1,2, 杨军^1,2, 王向东^1,2, 张罗^1,2,3*

1.首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,北京 100730;
2.北京市耳鼻咽喉科研究所,北京 100005;
3.中国医学科学院慢性鼻病诊疗策略研究创新单元,北京 100005

收稿日期:2022-09-06 出版日期:2022-12-21 发布日期:2022-11-30
基金资助:
教育部长江学者创新团队(IRT13082),中国医学科学院医学与健康科技创新工程项目(2019-I2M-5-022),北京市科技计划首都健康保障培育研究项目(Z181100001618002)。

Effect of baicalin on lipopolysaccharide-stimulated inflammatory response in human monocytes THP-1

Wang Ping^1,2, Yang Jun^1,2, Wang Xiangdong^1,2, Zhang Luo^1,2,3*

1. Department of Otorhinolaryngology Head and Neck Surgery and Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
2. Beijing Institute of Otorhinolaryngology, Beijing 100005, China;
3. Research Unit of Diagnosis and Treatment of Chronic Nasal Diseases, Chinese Academy of Medical Sciences, Beijing 100005, China

Received:2022-09-06 Online:2022-12-21 Published:2022-11-30
Contact: ^*E-mail:dr.luozhang@139.com

摘要/Abstract

摘要： 目的探讨黄芩苷(baicalin)对脂多糖(lipopolysaccharide, LPS)诱导的人单核细胞THP-1的炎症反应的作用。方法利用LPS建立人单核细胞THP-1的炎症模型,实时荧光定量聚合酶链式反应(real-time quantitatFive polymerase chain reaction, RT-qPCR)方法检测白细胞介素-1β(interleukin-1β,IL-1β)、IL-6、IL-8、粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony-stimulating factor,GM-CSF)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的相对表达,确定最优的LPS刺激浓度。乳酸脱氢酶检测试剂盒检测黄芩苷的细胞毒性,优选其最佳使用浓度范围。对LPS诱导的人单核细胞THP-1炎症模型予以不同浓度的黄芩苷预处理,利用RT-qPCR法检测黄芩苷对LPS诱导的人单核细胞THP-1的IL-1β、IL-6、IL-8、GM-CSF和TNF-α的表达升高的影响。结果与对照组相比,1 μg/mL的LPS处理人单核细胞THP-1 24 h后均可显著引起IL-1β、IL-6、IL-8、GM-CSF和TNF-α的表达升高(P<0.05),黄芩苷在浓度为10、30、100 μmol/L时处理人单核细胞THP-1 24 h和48 h,无明显细胞毒性。30 μmol/L和100 μmol/L的黄芩苷可降低LPS诱导的人单核细胞THP-1的IL-1β、IL-6、IL-8、GM-CSF和TNF-α的表达升高。结论黄芩苷可显著抑制LPS诱导的人单核细胞THP-1的炎症反应。

关键词: 黄芩苷, 脂多糖, 人单核细胞, 炎症反应, 细胞因子

Abstract: Objective To observe the effect of baicalin on the lipopolysaccharide (LPS)-stimulated inflammatory response in human monocytes THP-1. Methods LPS was used to induce the inflammatory response of human monocyte THP-1, and the relative expression levels of interleukin-1β (IL-1β), IL-6, IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor-α (TNF-α) were detected by real-time quantitative polymerase chain reaction (RT-qPCR) method to optimize the stimulation concentration of LPS.The cytotoxic effects of baicalein were also detected using the lactate dehydrogenase assay kit to optimize the bacailin concentration. The LPS-induced inflammation model of human monocyte THP-1 was pretreated with baicalin at different concentrations, and the inhibition effect of baicalin on the increasing expression of IL-1β, IL-6, IL-8, GM-CSF and TNF-α induced by LPS was examined by RT-qPCR. Results Compared to the control, the LPS treatment of human monocytes THP-1 at 1 μg/mL for 24 h could significantly increase the expression of IL-1β, IL-6, IL-8, GM-CSF and TNF-α (P<0.05).Treatment with baicalin at 10, 30 and 100 μmol/L on human monocytes THP-1 for 24 h and 48 h showed no significant cell cytotoxicity. The baicalein at 30 μmol/L and 100 μmol/L could significantly inhibit the LPS-induced increasing expression of IL-1β, IL-6, IL-8, GM-CSF and TNF-α in human monocytes THP-1. Conclusion The baicalin could significantly inhibit LPS-induced inflammatory response in human monocytes THP-1.

Key words: baicalin, lipopolysaccharide, human monocytes, inflammatory response, cytokine

中图分类号:

王平, 杨军, 王向东, 张罗. 黄芩苷对脂多糖诱导的人单核细胞THP-1的炎症反应的作用[J]. 首都医科大学学报, 2022, 43(6): 893-898.

Wang Ping, Yang Jun, Wang Xiangdong, Zhang Luo. Effect of baicalin on lipopolysaccharide-stimulated inflammatory response in human monocytes THP-1[J]. Journal of Capital Medical University, 2022, 43(6): 893-898.

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黄芩苷对脂多糖诱导的人单核细胞THP-1的炎症反应的作用

Effect of baicalin on lipopolysaccharide-stimulated inflammatory response in human monocytes THP-1

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