用32P标记的人乳头瘤病毒DNA检测宫颈病变组织DNA中病毒序列

首都医科大学学报 ›› 1989, Vol. 10 ›› Issue (4): 256-259.

用³²P标记的人乳头瘤病毒DNA检测宫颈病变组织DNA中病毒序列

刘晓明¹, 高天祥², 赵立新³

1. 山东医科大学电镜室;2. 首都医学院电镜室;3. 山东省人民医院妇产科

收稿日期:1989-02-15 修回日期:1900-01-01 出版日期:1989-10-15 发布日期:1989-10-15

Detection of Viral Sequences in DNA Extracted from Pathological Tissues of Carcinoma Cervix by Using ³²P-Labelled Human Papillomavirus DNA Probes

Liu Xiaoming¹, Gao Tianxiang², Zhao Lixin³

1. Laboratory of Electron Microscope, Shandong Medical University;2. Laboratory of Electron Microscope, Capital Institute of Medicine;3. Gynaecology and Obstetrics, Shandong People's Hospital

Received:1989-02-15 Revised:1900-01-01 Online:1989-10-15 Published:1989-10-15

摘要/Abstract

摘要： 使用Southern blot杂交方法对33例宫颈病变标本进行了检测,发现在26例宫颈癌中,6例存在HPV16DNA同源序列,占23%。HPV18同源序列占7.6%(2/26)。HPVX1同源序列为11.5%(3/26)。HPV16相关序列为27%(7/26)。而在4例慢性宫颈炎中仅有1例含HPV16相关序列,1例尖锐湿疣中含HPV16相关序列。

关键词: Scuthern blot杂交法, 人类乳头瘤病毒(HPV), 子宫颈癌

Abstract: Southern blot analysis of human papillomavirus genome was carried out on 33 cases of human cervical specimens histologically diagnosed as cervical cacinoma (26 cases), condyloma acuminatum (1 case), vulvar carcinoma (1 case) and chronic inflammation (4 cases). 69% of cervical carcinoma were found to be positive for HPV DNA. Among them 23% (6/26) were positive for HPV 16,7.6% (2/26) were positive for HPV 18 and 11.5% (3/26) were positive for HPV X1.

Key words: southern blot hybridization, human papillomaviruses(HPV), cervical carcinoma

刘晓明;高天祥;赵立新. 用³²P标记的人乳头瘤病毒DNA检测宫颈病变组织DNA中病毒序列[J]. 首都医科大学学报, 1989, 10(4): 256-259.

Liu Xiaoming;Gao Tianxiang;Zhao Lixin. Detection of Viral Sequences in DNA Extracted from Pathological Tissues of Carcinoma Cervix by Using ³²P-Labelled Human Papillomavirus DNA Probes[J]. Journal of Capital Medical University, 1989, 10(4): 256-259.

[1]	金凤羽, 阮祥燕, 杜娟, 程姣姣, 李扬璐, 王虎生, 谷牧青, 鞠蕊, 杨瑜, 王月姣, 许新. 中国首个卵巢组织冻存库冻存患者特征及冻存效果分析[J]. 首都医科大学学报, 2021, 42(4): 521-525.
[2]	盖其伟;赫翠珍;姚庆英;高天祥;周利华;刘晓明. 人类乳头瘤病毒16型DNA诱导NIH　3T3细胞的恶性转化[J]. 首都医科大学学报, 1994, 15(2): 109-111.

用³²P标记的人乳头瘤病毒DNA检测宫颈病变组织DNA中病毒序列

Detection of Viral Sequences in DNA Extracted from Pathological Tissues of Carcinoma Cervix by Using ³²P-Labelled Human Papillomavirus DNA Probes

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价