中国首个卵巢组织冻存库冻存患者特征及冻存效果分析

doi:10.3969/j.issn.1006-7795.2021.04.003

首都医科大学学报 ›› 2021, Vol. 42 ›› Issue (4): 521-525.doi: 10.3969/j.issn.1006-7795.2021.04.003

• 绝经、妇科内分泌与生育力保护 • 上一篇下一篇

中国首个卵巢组织冻存库冻存患者特征及冻存效果分析

金凤羽, 阮祥燕^*, 杜娟, 程姣姣, 李扬璐, 王虎生, 谷牧青, 鞠蕊, 杨瑜, 王月姣, 许新

首都医科大学附属北京妇产医院内分泌科,北京 100026

收稿日期:2021-05-18 出版日期:2021-08-21 发布日期:2021-07-29
基金资助:
北京市自然科学基金(7202047), 首都卫生发展科研专项资助(首发2020-2-2112),首都临床特色应用研究与成果推广(Z161100000516143),首都卫生发展科研专项项目(2016-2-2113),北京市医院管理局临床技术创新项目(XMLX201710),北京市医院管理局“登峰”计划专项(DFL20181401),北京市属医院科研培育计划项目(PZ2019031)。

Analysis on the characteristics of the patients and effects of ovarian tissue cryopreservation in the first ovarian tissues cryopreservation bank in China

Jin Fengyu, Ruan Xiangyan^*, Du Juan, Cheng Jiaojiao, Li Yanglu, Wang Husheng, Gu Muqing, Ju Rui, Yang Yu, Wang Yuejiao, Xu Xin

Department of Gynecological Endocrinology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China

Received:2021-05-18 Online:2021-08-21 Published:2021-07-29
Contact: * E-mail:ruanxiangyan@ccmu.edu.cn
Supported by:
Natural Science Foundation of Beijing (7202047); Capital's Funds for Health Improvement and Research (2020-2-2112); Beijing Municipal Science & Technology Commission (Z161100000516143); Capital's Funds for Health Improvement and Research (2016-2-2113); Beijing Municipal Administration of Hospitals Clinical Medicine, Development of Special Funding (XMLX201710); Beijing Municipal Administration of Hospitals' Ascent Plan (DFL20181401); Beijing Municipal Administration of Hospitals Incubating Program (PZ2019031).

摘要/Abstract

摘要： 目的评估中国首个卵巢组织冻存库进行卵巢组织冻存患者的特征及冻存效果。方法对首都医科大学附属北京妇产医院(中国首个卵巢组织冻存库)卵巢组织冻存患者数据库里面的217例女性患者的数据进行统计学分析,初步了解这部分人群所患疾病类型、年龄分布、所在城市、冻存前是否接受了放射治疗及化学药物治疗等情况。对其中的46位患者,每位患者取直径2 mm的标本共4个,采用数字表法随机选取其中2个用于新鲜卵巢组织的卵泡计数,剩余2个标本进行程序化冷冻/复苏,对比两组之间卵泡活性计数的差异。结果进行卵巢组织冻存患者的疾病类型以宫颈癌患者为主,占49.31%;年龄段主要在31～35岁,占总人数的36.4%,其次是26～30岁,占总人数的25.3%。新鲜卵巢组织组与冻存/复苏后卵巢组织组间卵泡活性计数差异无统计学意义(P>0.05)。结论首都医科大学附属北京妇产医院(中国首个卵巢组织冻存库)进行卵巢组织冻存患者的主要疾病类型是宫颈癌,人卵巢组织冻存/复苏技术能够有效保存患者生殖力。

关键词: 程序化冻存/复苏, 卵巢组织, 卵泡活性计数, 生殖力保存, 子宫颈癌

Abstract: Objective To evaluate the characteristics of the patients and effects of ovarian tissue cryopreservation in the first ovarian tissues cryopreservation bank in China. Methods The data of 217 female patients in the ovarian tissue cryopreservation database of Beijing Obstetrics and Gynecology Hospital (the first ovarian tissue cryopreservation bank in China) were statistically analyzed, including the diseases type characteristics, area, age distribution, and chemoradiotherapy received or not. Four specimens with diameter of 2 mm were taken from 46 patients, two of which were randomly selected for follicular count directly and the other two specimens were cryopreservation/thawing. The difference of follicular viability counts between the two groups was compared. Results The main disease type of ovarian tissue cryopreservation in Beijing Obstetrics and Gynecology Hospital is cervical cancer, accounting for 49.31%. The age group was mainly between 31 and 35 years old, accounting for 36.4% of the total number,following by age group between 26-30 years old, accounting for 25.3%. There was no significant difference in follicular viability between fresh tissue group and cryopreservation/thawing group (P>0.05). Conclusion The main diease type of ovarian tissue cryopreservation patients in Beijing Obstetrics and Gynecology Hospital is cervical cancer. Our ovarian cryopreservation technique can effectively preserve patients' fertility.

Key words: programed cryopreservation/thawing, ovarian tissue, follicle counts, fertility protection, cervical cancer

中图分类号:

R711.75

金凤羽, 阮祥燕, 杜娟, 程姣姣, 李扬璐, 王虎生, 谷牧青, 鞠蕊, 杨瑜, 王月姣, 许新. 中国首个卵巢组织冻存库冻存患者特征及冻存效果分析[J]. 首都医科大学学报, 2021, 42(4): 521-525.

Jin Fengyu, Ruan Xiangyan, Du Juan, Cheng Jiaojiao, Li Yanglu, Wang Husheng, Gu Muqing, Ju Rui, Yang Yu, Wang Yuejiao, Xu Xin. Analysis on the characteristics of the patients and effects of ovarian tissue cryopreservation in the first ovarian tissues cryopreservation bank in China[J]. Journal of Capital Medical University, 2021, 42(4): 521-525.

参考文献

[1] Donnez J, Dolmans M M, Demylle D, et al. Restoration of ovarian function after orthotopic (intraovarian and periovarian) transplantation of cryopreserved ovarian tissue in a woman treated by bone marrow transplantation for sickle cell anaemia: case report[J]. Hum Reprod, 2006,21(1): 183-188.
[2] Donnez J, Squifflet J, Van Eyck A S, et al. Restoration of ovarian function in orthotopically transplanted cryopreserved ovarian tissue: a pilot experience[J]. Reprod Biomed Online, 2008,16(5): 694-704.
[3] Wallace W H, Smith A G, Kelsey T W, et al. Fertility preservation for girls and young women with cancer: population-based validation of criteria for ovarian tissue cryopreservation[J]. Lancet Oncol, 2014,15(10):1129-1136.
[4] Donnez J, Dolmans M M. Fertility preservation in women[J]. N Engl J Med, 2017,377(17):1657-1665.
[5] Ruan X, Du J, Korell M, et al. Case report of the first successful cryopreserved ovarian tissue retransplantation in China[J]. Climacteric, 2018,21(6):613-616.
[6] 阮祥燕,杜娟,卢丹,等.中国自体卵巢组织冻存移植后成功妊娠首例报道[J].中国临床医生杂志,2021,49(3):375-378.
[7] Isachenko V, Isachenko E, Reinsberg J, et al. Cryopreservation of human ovarian tissue: comparison of rapid and conventional freezing[J]. Cryobiology, 2007,55(3):261-268.
[8] Dittrich R, Kliesch S, Schüring A, et al. Fertility preservation for patients with malignant disease. Guideline of the DGGG, DGU and DGRM (S2k-Level, AWMF Registry No.015/082, November 2017)-Recommendations and Statements for Girls and Women[J]. Geburtsh Frauenheilk, 2018,78(6):567-584.
[9] Torino F, Barnabei A, De Vecchis L, et al.Chemotherapy-induced ovarian toxicity in patients affected by endocrine-responsive early breast cancer[J].Crit Rev Oncol Hematol, 2014, 89(1): 27-42.
[10] von Wolff M, Dittrich R, Liebenthron J, et al. Fertility-preservation counselling and treatment for medical reasons: data from a multinational network of over 5000 women[J]. Reprod Biomed Online, 2015,31(5):605-612.
[11] 阮祥燕,杜娟,卢丹,等.中国首例冻存卵巢组织移植报告[J].首都医科大学学报,2016,37(6): 840-842.
[12] 阮祥燕, Alfred O Mueck.妇科内分泌病例评析[M].北京:人民卫生出版社, 2016:161-162.
[13] Lobke B, Westphal J R,Beerendonk C C, et al. Clinically applied procedures for human ovarian tissue cryopreservation result in different levels of efficacy and efficiency [J]. J Assist Reprod Genet,2016,33(12):1605-1614.
[14] Bastings L, Liebenthron J, Westphal J R, et al. Efficacy of ovarian tissue cryopreservation in a major European center[J]. J Assist Reprod Genet,2014,31(8):1003-1012.

中国首个卵巢组织冻存库冻存患者特征及冻存效果分析

Analysis on the characteristics of the patients and effects of ovarian tissue cryopreservation in the first ovarian tissues cryopreservation bank in China

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