二氧化硫对自发性高血压大鼠主动脉平滑肌细胞内质网应激的影响

首都医科大学学报 ›› 2010, Vol. 31 ›› Issue (2): 183-186.

二氧化硫对自发性高血压大鼠主动脉平滑肌细胞内质网应激的影响

朱露璐¹, 赵霞¹, 唐朝枢^2,3, 杜军保^1,3, 金红芳^1*

1. 北京大学第一医院儿科;2. 北京大学医学部生理与病理生理教研室;3. 北京大学教育部分子心血管重点实验室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-04-21 发布日期:2010-04-21
通讯作者: 金红芳

Impact of Sulfur Dioxide on the Endoplasmic Reticulum Stress of Aortic Smooth Muscle Cells in the Spontaneously Hypertensive Rats

ZHU Lu-lu¹, ZHAO Xia¹, TANG Chao-shu^2,3, DU Jun-bao^1,3, JIN Hong-fang^1*

1. Department of Pediatrics, Peking University First Hospital;2. Department of Physiology and Pathophysiology, Peking University Health Science Center; 3. Key Laboratory of Molecular Cardiovascular Diseases, Ministry of Education

Received:1900-01-01 Revised:1900-01-01 Online:2010-04-21 Published:2010-04-21
Contact: JIN Hong-fang

摘要/Abstract

摘要： 目的探讨二氧化硫(sulfur dioxide，SO₂)对自发性高血压大鼠（spontaneously hypertensive rat，SHR）主动脉平滑肌细胞内质网应激的调节作用。方法 4周龄正常血压WKY（wistar-kyoto rat）大鼠7只作为WKY对照组。4周龄SHR大鼠12只分为SHR（spontaneously hypertensive rat）对照组及SHR+SO₂供体(Na₂SO₃/NaHSO₃)组,每组6只。5周后检测大鼠血压，采用高效液相色谱（HPLC）法测定血浆SO₂水平，采用免疫组织化学方法检测主动脉平滑肌细胞GRP78和caspase-12的蛋白表达。结果 9周时SHR对照组血压明显高于WKY对照组，血浆SO₂水平显著低于WKY对照组，主动脉平滑肌细胞GRP78和caspase-12蛋白表达显著增高。与SHR对照组比较，SHR+SO₂供体组大鼠血压明显降低，血浆SO₂含量升高，主动脉平滑肌细胞GRP78和caspase-12蛋白表达降低。结论 SO₂可能抑制高血压大鼠主动脉血管平滑肌细胞内质网应激反应的激活。

关键词: 二氧化硫, 高血压, 主动脉, 内质网应激

Abstract: Objective To explore the effect of sulfur dioxide(SO₂) on the endoplasmic reticulum stress(ERS) in the aortic smooth muscle cells of spontaneously hypertensive rat(SHR). Methods Seven male WKY rats were used as WKY control group. Twelve male SHRs at the age of 4 weeks were randomly divided into SHR control group and SHR+SO₂ donor(Na₂SO₃/NaHSO₃) group, respectively. Five weeks later, the blood pressure was determined. SO₂ content in the plasma was detected by reversephase high performance liquid chromatography with fluorescence detection(HPLC). The expressions of glucose-regulated protein 78(GRP78) and caspase-12 in the aortic smooth muscle cells were measured by immuohistochemistry. Results Compared with WKY control group, SO₂ contents in the plasma were decreased, while the blood pressure and the expressions of GRP78 and caspase-12 in the aortic smooth muscle cells were increased in the rats of SHR control group significantly, respectively. Administration of SO₂ donor increased the SO₂ content in the plasma in the rats of SHR control group. Moreover, SO₂ donor decreased the blood pressure and the expressions of GRP78 and caspase-12 in the aortic smooth muscle cells of SHR control rats. Conclusion SO₂ might inhibit the activation of ERS in the aortic smooth muscle cells of hypertensive rats.

Key words: sulfur dioxide, hypertension, aortic, endoplasmic reticulum stress

中图分类号:

R 544.1

朱露璐;赵霞;唐朝枢;杜军保;金红芳. 二氧化硫对自发性高血压大鼠主动脉平滑肌细胞内质网应激的影响[J]. 首都医科大学学报, 2010, 31(2): 183-186.

ZHU Lu-lu;ZHAO Xia;TANG Chao-shu;DU Jun-bao;JIN Hong-fang. Impact of Sulfur Dioxide on the Endoplasmic Reticulum Stress of Aortic Smooth Muscle Cells in the Spontaneously Hypertensive Rats[J]. Journal of Capital Medical University, 2010, 31(2): 183-186.

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