酶法与Jaffe反应速率法测定肌酐的比较及干扰因素分析

首都医科大学学报 ›› 2002, Vol. 23 ›› Issue (4): 344-345.

酶法与Jaffe反应速率法测定肌酐的比较及干扰因素分析

金晔, 苏建荣

首都医科大学附属北京友谊医院检验科

收稿日期:2001-11-30 修回日期:1900-01-01 出版日期:2002-10-15 发布日期:2002-10-15

Comparison Between Creatinine's Enzymatic Method and Kinetic Jaffe's Method and Interference Factors Analysis

Jin Ye, Su Jianrong

Department of Laboratory, Beijing Friendship Hospital, Affiliate of Capital University of Medical Sciences

Received:2001-11-30 Revised:1900-01-01 Online:2002-10-15 Published:2002-10-15

摘要/Abstract

摘要： 为比较酶法与Jaffe反应速率法测定肌酐的结果及分析其干扰因素,对酶法、Jaffe反应速率法测定肌酐进行方法学评价,在BeckmanCX-7上分析测定结果,并且分析黄疸、溶血、血脂对测定结果的干扰.结果酶法基本不受干扰物影响,Jaffe反应速率法受干扰物影响较大,胆红素>158μmol/L时有明显负干扰.提示:酶法是目前测定肌酐的最好方法.

关键词: 肌酐, 酶法, Jaffe, 干扰因素

Abstract: The objective was to compare and investigate the interference and the difference of Creatinine's enzymatic method and kinetic Jaffe method. To measure creatinine of serum or plasma, Beckman CX 7 analyzer with Beckman reagent for kinetic Jaffe method and Randox reagent for enzymatic method were used. The interferences of lipemia, hemoglobin and bilirubin were compared. The results showed that enzymatic method was not interfered, Jaffe method was negatively affected by bilirubin(>158 μmol/L). Creatinine's enzymatic method is better than kinetic Jaffe method.

Key words: creatinine|enzyme method|Jaffe|interference

中图分类号:

R446.11

金晔;苏建荣. 酶法与Jaffe反应速率法测定肌酐的比较及干扰因素分析[J]. 首都医科大学学报, 2002, 23(4): 344-345.

Jin Ye;Su Jianrong . Comparison Between Creatinine's Enzymatic Method and Kinetic Jaffe's Method and Interference Factors Analysis[J]. Journal of Capital Medical University, 2002, 23(4): 344-345.

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