MT启动子控制的GFP基因在CHO细胞中的诱导表达

首都医科大学学报 ›› 2003, Vol. 24 ›› Issue (2): 118-120.

MT启动子控制的GFP基因在CHO细胞中的诱导表达

孙丽翠¹, 祁雅慧¹, 仲峰²

1. 首都医科大学实验中心;2. 张家口老虎头煤矿职工医院

收稿日期:2002-06-17 修回日期:1900-01-01 出版日期:2003-04-15 发布日期:2003-04-15

Inducible Expression of GFP Gene in CHO Cells under Control of MT Promoter

Sun Licui¹, Qi Yahui¹, Zhong Feng²

1. Department of Experiment Centre, Capital University of Medical Sciences Laohutou Colliery Hospital of Zhangjiakou;2. Laohutou Colliery Hospital of Zhangjiakou

Received:2002-06-17 Revised:1900-01-01 Online:2003-04-15 Published:2003-04-15

摘要/Abstract

摘要： 用羊金属硫蛋白(MT)启动子构建含绿色荧光蛋白(GFP)报告基因的真核表达载体,经脂质体介导在培养的中国仓鼠卵巢(CHO)细胞中进行诱导性表达。结果:构建的GFP基因表达载体能在CHO细胞中进行表达,用硫酸锌诱导可提高GFP的表达量。

关键词: MT启动子, 诱导表达, GFP报告基因

Abstract: Eukaryotic expression vector of GFP report gene under the control of sheep MT promoter was constructed. Result showed that the GFP gene in the vector could express itself inducibly in cultured CHO cells. The expression level of GFPcould be increased by the inducement of ZnSO₄.

Key words: MT promoter, inducible expression, GFP report gene

中图分类号:

Q786

孙丽翠;祁雅慧;仲峰. MT启动子控制的GFP基因在CHO细胞中的诱导表达[J]. 首都医科大学学报, 2003, 24(2): 118-120.

Sun Licui;Qi Yahui;Zhong Feng. Inducible Expression of GFP Gene in CHO Cells under Control of MT Promoter[J]. Journal of Capital Medical University, 2003, 24(2): 118-120.

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