APP17肽对糖尿病大鼠海马线粒体膜电位的影响

首都医科大学学报 ›› 2003, Vol. 24 ›› Issue (1): 40-43.

APP17肽对糖尿病大鼠海马线粒体膜电位的影响

李红星², 王蓉¹, 杜怡峰¹, 姬志娟¹, 盛树力¹

1. 首都医科大学宣武医院脑老化研究实验室;2. 首都医科大学

收稿日期:2002-09-27 修回日期:1900-01-01 出版日期:2003-01-15 发布日期:2003-01-15
通讯作者: 盛树力

Effect of APP-17 Peptide on Modulating Neuronal Mitochondria Transmembrane Potentials in Hippocampus of STZ-DM Rats

Li Hongxing², Wang Rong¹, Du Yifeng¹, Ji Zhijuan¹, Sheng Shuli¹

1. Beijing Research Laboratory for Brain Aging, Xuanwu Hospital, Affiliate of Capital University of Medical Sciences;2. Capital University of Medical Sciences

Received:2002-09-27 Revised:1900-01-01 Online:2003-01-15 Published:2003-01-15

摘要/Abstract

摘要： 应用流式细胞仪分别观察糖尿病模型组、糖尿病APP17肽治疗组和正常对照组大鼠海马线粒体膜电位和凋亡率。结果:糖尿病组大鼠海马线粒体膜电位明显下降(P<0.01),凋亡发生率增加(P<0.01);APP17肽治疗组与糖尿病组相比海马线粒体膜电位增高(P<0.05),凋亡发生率降低(P<0.05),与正常对照组比较无显着性差异。结果提示:海马线粒体膜电位下降和细胞的凋亡可能参与糖尿病脑病的发生和发展;APP17肽具有改善这一病理过程的作用。

关键词: APP17, 糖尿病脑病, 线粒体膜电位, 凋亡, 海马

Abstract: The objectives were to investigate the role of the alteration of mitochondria transmembrane potentials in apoptosis in hippocampal neurons of STZ-DM rats and the possible effect of APP17 peptide on it. DM models were established by injection of streptozotocin intraperitoneal, the APP17 peptide treated group were injected with APP17 peptide three times a week, whereas the control and DM groups were given saline. Single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the percentage of cell in apoptosis were measured by means of flow cytometry (FCM) at the 11th week when the animals were sacrificed. The neurons in hippocampus of DM group had lower mitochondria transmembrane potentials and higher apoptotic rate than those of the control group. APP-17 peptide could significantly blocked the mitochondria transmembrane potentials collapse and apoptosis. Decreased mitochondria transmembrane potential and related apoptosis may contribute to the mechanism of diabetic encephalopathy. APP-17 peptide palys an anti apoptosis role in it.

Key words: APP-17, peptide diabetic, encephalopathy, mitochondria transmembrane potential, apoptosis, hippocampus

中图分类号:

R587.1

李红星;王蓉;杜怡峰;姬志娟;盛树力. APP17肽对糖尿病大鼠海马线粒体膜电位的影响[J]. 首都医科大学学报, 2003, 24(1): 40-43.

Li Hongxing;Wang Rong;Du Yifeng;Ji Zhijuan;Sheng Shuli. Effect of APP-17 Peptide on Modulating Neuronal Mitochondria Transmembrane Potentials in Hippocampus of STZ-DM Rats[J]. Journal of Capital Medical University, 2003, 24(1): 40-43.

[1]	李扬璐, 阮祥燕, 杜娟, 程姣姣, 金凤羽, 谷牧青, 李妍秋, Alfred O. Mueck. 人卵巢组织冻融过程对卵巢组织的影响[J]. 首都医科大学学报, 2025, 46(4): 594-600.
[2]	李玥, 陈俊逾, 常春雷, 张静. 基于NLRP3/IL-18/NF-κB通路探讨化痰安神方改善神经元细胞凋亡的作用机制[J]. 首都医科大学学报, 2025, 46(3): 487-495.
[3]	何南, 王亚龙. 益生菌对大鼠热性惊厥发作及海马组织中NOD样受体热蛋白结构域相关蛋白3炎症小体活化的影响[J]. 首都医科大学学报, 2025, 46(3): 538-544.
[4]	苟东芸, 梁加一, 郭梓煜, 闫方圆, 王帅翔, 渠硕, 高雁, 张雨佳, 马晓伟. 脑蛋白水解物通过p38 MAPK信号通路降低阿尔茨海默病小鼠的细胞凋亡、炎症及氧化应激水平[J]. 首都医科大学学报, 2024, 45(6): 1062-1070.
[5]	刘秀, 李文才. UNC0379对胶质瘤细胞增殖影响的初步探索[J]. 首都医科大学学报, 2023, 44(5): 741-746.
[6]	于建宇, 郑义, 李文斌. 海马钙素类1在原发性中枢神经系统淋巴瘤中表达和临床预后的生物信息学分析[J]. 首都医科大学学报, 2023, 44(5): 724-732.
[7]	周妍, 吴巍. 萝卜硫素干扰微管与线粒体动力学抗肿瘤机制[J]. 首都医科大学学报, 2023, 44(3): 363-369.
[8]	杨一凡, 王雅梅. 凋亡诱导因子2在乳腺癌中的表达及其相关作用[J]. 首都医科大学学报, 2023, 44(2): 248-257.
[9]	张莹, 刘超楠, 时红林, 刘芳, 陈德喜, 时红波. P53凋亡刺激蛋白2介导酒精性肝病脂代谢相关基因的转录组学分析[J]. 首都医科大学学报, 2023, 44(1): 85-92.
[10]	刘志斌, 靳松, 牛亦农. 苯乙基异硫氰酸酯对良性前列腺上皮细胞增殖凋亡的影响及相关机制研究[J]. 首都医科大学学报, 2021, 42(6): 961-966.
[11]	安松林, 马玲, 赵月, 李雁. 主要促进因子超家族成员MFSD2A对肝癌细胞增殖、凋亡和侵袭的影响[J]. 首都医科大学学报, 2021, 42(6): 1000-1006.
[12]	崔兴, 钟萍. 我的梦想是做“百年老店”——记首都医科大学三博脑科医院神经外科于春江教授[J]. 首都医科大学学报, 2021, 42(1): 167-169.
[13]	刘妍, 陈玉静, 陈文强, 黄小波. 远志汤对慢性脑低灌注大鼠认知功能及海马凋亡蛋白的影响[J]. 首都医科大学学报, 2019, 40(3): 323-329.
[14]	张怡尘, 曹曦, 杨金奎. G蛋白偶联受体MAS对肝脏糖脂代谢的影响[J]. 首都医科大学学报, 2019, 40(3): 409-416.
[15]	卢晶, 沈涵, 程呈, 刘敬怡, 朱晓蓉, 谢荣荣, 袁明霞, 杨金奎. 人ether-α-go-go钾通道减少肝脏的内质网应激和凋亡[J]. 首都医科大学学报, 2019, 40(1): 45-52.