APP17肽对糖尿病大鼠海马线粒体膜电位的影响

首都医科大学学报 ›› 2003, Vol. 24 ›› Issue (1): 40-43.

APP17肽对糖尿病大鼠海马线粒体膜电位的影响

李红星², 王蓉¹, 杜怡峰¹, 姬志娟¹, 盛树力¹

1. 首都医科大学宣武医院脑老化研究实验室;2. 首都医科大学

收稿日期:2002-09-27 修回日期:1900-01-01 出版日期:2003-01-15 发布日期:2003-01-15
通讯作者: 盛树力

Effect of APP-17 Peptide on Modulating Neuronal Mitochondria Transmembrane Potentials in Hippocampus of STZ-DM Rats

Li Hongxing², Wang Rong¹, Du Yifeng¹, Ji Zhijuan¹, Sheng Shuli¹

1. Beijing Research Laboratory for Brain Aging, Xuanwu Hospital, Affiliate of Capital University of Medical Sciences;2. Capital University of Medical Sciences

Received:2002-09-27 Revised:1900-01-01 Online:2003-01-15 Published:2003-01-15

摘要/Abstract

摘要： 应用流式细胞仪分别观察糖尿病模型组、糖尿病APP17肽治疗组和正常对照组大鼠海马线粒体膜电位和凋亡率。结果:糖尿病组大鼠海马线粒体膜电位明显下降(P<0.01),凋亡发生率增加(P<0.01);APP17肽治疗组与糖尿病组相比海马线粒体膜电位增高(P<0.05),凋亡发生率降低(P<0.05),与正常对照组比较无显着性差异。结果提示:海马线粒体膜电位下降和细胞的凋亡可能参与糖尿病脑病的发生和发展;APP17肽具有改善这一病理过程的作用。

关键词: APP17, 糖尿病脑病, 线粒体膜电位, 凋亡, 海马

Abstract: The objectives were to investigate the role of the alteration of mitochondria transmembrane potentials in apoptosis in hippocampal neurons of STZ-DM rats and the possible effect of APP17 peptide on it. DM models were established by injection of streptozotocin intraperitoneal, the APP17 peptide treated group were injected with APP17 peptide three times a week, whereas the control and DM groups were given saline. Single cell of hippocampus was isolated, and mitochondria transmembrane potentials and the percentage of cell in apoptosis were measured by means of flow cytometry (FCM) at the 11th week when the animals were sacrificed. The neurons in hippocampus of DM group had lower mitochondria transmembrane potentials and higher apoptotic rate than those of the control group. APP-17 peptide could significantly blocked the mitochondria transmembrane potentials collapse and apoptosis. Decreased mitochondria transmembrane potential and related apoptosis may contribute to the mechanism of diabetic encephalopathy. APP-17 peptide palys an anti apoptosis role in it.

Key words: APP-17, peptide diabetic, encephalopathy, mitochondria transmembrane potential, apoptosis, hippocampus

中图分类号:

R587.1

李红星;王蓉;杜怡峰;姬志娟;盛树力. APP17肽对糖尿病大鼠海马线粒体膜电位的影响[J]. 首都医科大学学报, 2003, 24(1): 40-43.

Li Hongxing;Wang Rong;Du Yifeng;Ji Zhijuan;Sheng Shuli. Effect of APP-17 Peptide on Modulating Neuronal Mitochondria Transmembrane Potentials in Hippocampus of STZ-DM Rats[J]. Journal of Capital Medical University, 2003, 24(1): 40-43.

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