首都医科大学学报 ›› 2004, Vol. 25 ›› Issue (1): 57-59.

• 论著·临床研究 • 上一篇    下一篇

免疫荧光染色检测不同组织细胞微管蛋白-β的表达

历俊华, 万虹, 王忠诚   

  1. 北京市神经外科研究所
  • 收稿日期:2003-04-06 修回日期:1900-01-01 出版日期:2004-01-15 发布日期:2004-01-15

Tubulin-β Expression of Various Culture Cells Using Immunoinfluorescence

Li Junhua, Wan Hong, Wang Zhongcheng Beijing Neurosurgical Institute   

  1. Beijing Neurosurgical Institute
  • Received:2003-04-06 Revised:1900-01-01 Online:2004-01-15 Published:2004-01-15

摘要: 为了探讨体外培养的正常及肿瘤细胞微管蛋白-β的表达,将肺成纤维细胞(3T3)、胃癌细胞(BGC-823)、神经母细胞瘤株(SH-SY5Y)、大鼠胶质瘤细胞株(C6)、多形性胶质母细胞瘤株(BT325)体外培养,免疫荧光细胞染色检测微管蛋白-β的表达。发现:所有细胞均为微管蛋白-β免疫反应阳性。结果提示:微管蛋白β阳性反应可发生在增生活跃的培养细胞.

关键词: 微管蛋白-β, 免疫荧光, 增生

Abstract: To explore tubulin-β expression of normal and tumor cells in vitro, 3T3, BGC-823, SH-SY5Y, C6, BT325 were cultured in vitro and immunofluorescent staining was used to investigate tubulin-β immunoreactivity. Results showed that the po sitive cells were intense red in body and processes which presented in all of cells. The findings prove that tubulin-β expression may occur in any multipl ication cell including normal cells and tumor cells.

Key words: tubulin-β, immunofluorescence, multiplication

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