脑细胞悬液移植治疗黑质损伤 Ⅰ 黑质损伤动物模型的建立

首都医科大学学报 ›› 1986, Vol. 7 ›› Issue (2): 107-109,106,167.

脑细胞悬液移植治疗黑质损伤 Ⅰ 黑质损伤动物模型的建立

邵宴平, 焦守恕, 王元身, 陈增

北京第二医学院解剖学教研室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1986-04-15 发布日期:1986-04-15

Intracerebral Grafting of Neuronal Cell Suspensions Correct the Damage of Substantia Nigra Ⅰ. Animal Model

Shao Yanping

Department of Anatomy

Received:1900-01-01 Revised:1900-01-01 Online:1986-04-15 Published:1986-04-15

摘要/Abstract

摘要：

本文应用67只Wistar种系大鼠在左侧注射6-OHDA后引起黑质纹体通路的完全损毁。术后一个月始,给动物注射Apomorphine(0.5mg/kg)诱发旋转行为并利用自制的计算机控制的旋转记录仪记录。平均转速达7圈/分钟者(旋转360°算一圈)判为模型成功。32.8%的实验动物恒定地向右旋转。T型迷宫试验显示实验动物多有左侧空间选择偏向。脑组织切片可见实验动物的左侧黑质区变性,用SPG荧光组化法观察该区内未见DA细胞,同侧尾状核内亦未见DA未稍。

Abstract:

The study was based upon observations of 67 adult wistar rats sustained 6-OHDA induced complete destruction of the nigrostriatal DA pathway on the left side. Rotation tests were conducted in a rotometer bowls with computer built-in designed by ourselves. Tests were initiated approximately one month after severing this Pathway.The animals were given 0.5 mg/kg of apomorphine hydrochloride that evoked consistent turning of the animal. An average turning rate of more than 7 cycle/minute (a full 360-degree turn as one cycle) was considered to meet the criterion for a succesful animal model. 32.8% of the experimental animals turned to the right consistently, contralateral to the lesion. T-maze tests showed that most of the experimental animals had a predilection for left-sided deviation. Brain sections of the experimental animals showed degernerative changes in left SN area, and no DA cell was seen there by histochemistry method SPG, also no DA terminals in the ipsilatetal caudate nucleus area were detectable.

邵宴平;焦守恕;王元身;陈增. 脑细胞悬液移植治疗黑质损伤 Ⅰ 黑质损伤动物模型的建立[J]. 首都医科大学学报, 1986, 7(2): 107-109,106,167.

Shao Yanping. Intracerebral Grafting of Neuronal Cell Suspensions Correct the Damage of Substantia Nigra Ⅰ. Animal Model[J]. Journal of Capital Medical University, 1986, 7(2): 107-109,106,167.