脑细胞悬液移植治疗黑质损伤 Ⅱ 悬液制备、移植物成活、生长和形态

首都医科大学学报 ›› 1986, Vol. 7 ›› Issue (2): 110-112,167,168.

脑细胞悬液移植治疗黑质损伤 Ⅱ 悬液制备、移植物成活、生长和形态

焦守恕, 邵宴平, 王元身, 陈增

北京第二医学院解剖学教研室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1986-04-15 发布日期:1986-04-15

Intracerebral Grafting of Neuronal Cell Suspensions Correct the Damage of Substantia Nigra Ⅱ. Suspension Preperation, Survial, Growth and Structure of the Grafts

Jiao Shoushu

Department of Anatomy

Received:1900-01-01 Revised:1900-01-01 Online:1986-04-15 Published:1986-04-15

摘要/Abstract

摘要：

从12～15天的胚胎中脑腹侧部取材用以制备含DA细胞的悬液(供体和受体动物均用Wistar大鼠)。选经6-OHDA损毁已存活6个月的动物,向其伤侧尾状核内单点或多点注射细胞悬液(3—4万个细胞/μl)5～40μl(5μl/点)。14个受体动物中有10个动物的脑内移植物成活。多数移植物内含有大量成活的DA细胞体,在注射部位聚集成若干细胞团簇。尾状核接受多点移植的动物中,移植物的体积较小,DA细胞的含量也较少。移植物附近的受体脑组织中可见大量DA末梢。本文结果显示,胚胎黑质组织的细胞悬液移植可以改善因6-OHDA损毁DA通路造成的行为异常。多点悬液移植物比单个移植物对行为的恢复更为有效。

Abstract:

DA-rich cell suspensions were obtained from tissue dissected from the ventral mesencephalon of 12-15 day embryos (donor and host, wistar rats) ,6months after 6-OHDA lesion, 5-40 μl of the suspension (30,000-40,000 cells/μl) was implanted at one or several sites (5μl/site) in the caudate nucleus, iPsilateral to the lesion. Overall, about 10 out of 14 implants survived.Most of the implants contained a lot of surviving DAcontaining cell bodies, arranged in clusters at the site of injection. In animals with multi-focal implantation in the caudate necleus, the grafts seemed to be smaller in size and contain fewer DA neurons. A number of DA terminals were found in the neighbouring host brain tissue. The results indicate that suspension grafts of embryonic nigral tissue can ameliorate behavioural impairments induced by dopamine-depleting 6-OHDA lesions. Multi-focal injections of suspension grafts were found to provide a more extensive behavioural recovery as compared with rats receiving only a single graft.

焦守恕;邵宴平;王元身;陈增. 脑细胞悬液移植治疗黑质损伤 Ⅱ 悬液制备、移植物成活、生长和形态[J]. 首都医科大学学报, 1986, 7(2): 110-112,167,168.

Jiao Shoushu. Intracerebral Grafting of Neuronal Cell Suspensions Correct the Damage of Substantia Nigra Ⅱ. Suspension Preperation, Survial, Growth and Structure of the Grafts[J]. Journal of Capital Medical University, 1986, 7(2): 110-112,167,168.