分光光度法测定仙鹤草总酚含量的方法研究

doi:10.3969/j.issn.1006-7795.2012.01.022

首都医科大学学报 ›› 2012, Vol. 33 ›› Issue (1): 107-110.doi: 10.3969/j.issn.1006-7795.2012.01.022

分光光度法测定仙鹤草总酚含量的方法研究

李倩¹, 刘子衿¹, 叶燕彬², 夏颖², 张枫²

1. 首都医科大学基础医学院, 北京 100069;2. 首都医科大学化学生物学与药学院, 北京 100069

收稿日期:2011-09-23 修回日期:1900-01-01 出版日期:2012-02-21 发布日期:2012-02-21
通讯作者: 张枫

Study on determining of total polyphenols content in agrimonia pilosa ledeb. by the spectrophotometry

LI Qian¹, LIU Zi-jin¹, YE Yan-bin², XIA Ying², ZHANG Feng²

1. School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;2. School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China

Received:2011-09-23 Revised:1900-01-01 Online:2012-02-21 Published:2012-02-21

摘要/Abstract

摘要： 目的建立测定仙鹤草中总酚含量的测定方法。方法通过可见分光光度法,以没食子酸为标准品,采用Folin-Ciocalteus试剂,在装有仙鹤草样品的10mL容量瓶中,依次加入Folin-Ciocalteus试剂0.5mL,20%Na₂CO₃1.7mL,在室温条件下放置90min,然后在波长760nm和660nm测定吸光度。结果总多酚浓度在0~10.4 mg/L与吸光度有良好的线性关系。760nm时的回归方程为y=0.091.8x+0.012.9,R²=0.999 3,加标回收率为98.4%~99.8%;660nm时的回归方程为y=0.084 7x-0.002 6,R²=0.999 7,加标回收率为101.2%~101.9%。结论在760nm和660nm 2个波长处测定的结果,都具有良好的精密度、重复性和回收率,可以作为测定仙鹤草多酚定量分析的参考方法。

关键词: 仙鹤草, 分光光度法, Folin-Ciocalteus试剂, 植物多酚

Abstract: Objective To propose a method to determine the total polyphenols in agrimonia pilosa ledeb. Methods Folin-Ciocalteus reagent and gallic acid as the standard were used to determine the total polyphenols in agrimonia pilosa ledeb by the spectrophotometry. 0.5mL of Folin-Ciocalteus reagent and 1.7mL of 20% Na₂CO₃ solution were added into 10mL volumetric flask which contained the certain amounts of agrimonia pilosa ledeb sample. The reaction continued of 90 min at room temperature, and then determined absorption value at 660nm and 760nm. Results The mass concentrations of polyphenols in the interval of 0~10.4 mg/L were linearly related to the absorption values. The standard curve at 760nm was y=0.091 8x+0.012 9, R²=0.999 3 and the recovery of total polyphenols was 98.4%~99.8%. The standard curve at 660nm was y=0.084 7x-0.002 6, R²=0.999 7 and the recovery of total polyphenols was 101.2%~101.9%. Conclusion It showed good precision, repeatability and recovery to determine total polyphenols both at 760 nm and 660nm. This research provided a reference for quantitative analysis total polyphenols of agrimonia pilosa ledeb.

Key words: agrimonia pilosa ledeb, spectrophotometry, Folin-Ciocalteus reagent, plant polyphenol

中图分类号:

李倩;刘子衿;叶燕彬;夏颖;张枫. 分光光度法测定仙鹤草总酚含量的方法研究[J]. 首都医科大学学报, 2012, 33(1): 107-110.

LI Qian;LIU Zi-jin;YE Yan-bin;XIA Ying;ZHANG Feng. Study on determining of total polyphenols content in agrimonia pilosa ledeb. by the spectrophotometry[J]. Journal of Capital Medical University, 2012, 33(1): 107-110.

分光光度法测定仙鹤草总酚含量的方法研究

Study on determining of total polyphenols content in agrimonia pilosa ledeb. by the spectrophotometry

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