妊娠晚期维生素C干预对大鼠先天性膈疝模型中胎肺的作用

doi:10.3969/j.issn.1006-7795.2012.02.021

首都医科大学学报 ›› 2012, Vol. 33 ›› Issue (2): 238-241.doi: 10.3969/j.issn.1006-7795.2012.02.021

妊娠晚期维生素C干预对大鼠先天性膈疝模型中胎肺的作用

蔡晶晶¹, 陈永卫², 张建敏¹

1. 首都医科大学附属北京儿童医院麻醉科,北京 100045;2. 首都医科大学附属北京儿童医院外科,北京 100045

收稿日期:2010-07-12 修回日期:1900-01-01 出版日期:2012-04-21 发布日期:2012-04-21
通讯作者: 张建敏

The effect of vitamin C given in late gestation on the lung of fetal rat with congenital diaphragmatic hernia

CAI Jing-jing¹, CHEN Yong-wei², ZHANG Jian-min¹

1. Department of Anesthesiology, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China;2. Department of Surgery, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China

Received:2010-07-12 Revised:1900-01-01 Online:2012-04-21 Published:2012-04-21

摘要/Abstract

摘要： 目的建立大鼠先天性膈疝(congenital diaphragmatic hernia,CDH)模型,检验妊娠晚期维生素C干预对先天性膈疝的发生率以及胎肺发育的作用。方法妊娠S-D雌鼠12只采用抽签法随机分为4组,对照组(C)、维生素C组(W)、膈疝组(N)及治疗组(T)。N、T组除草醚(nitrofen)灌胃建立胎鼠先天性膈疝模型;孕晚期W、T组维生素C干预。正常孕期前一天(孕21天)剖腹取出胎鼠,记录体质量及肺脏质量;检查胎鼠膈疝的发生率、发生部位、膈肌缺损程度;采用免疫组织化学技术显示ki-67的表达,计算机软件分析计算胎肺组织中增生细胞的比例。结果膈疝组与治疗组膈疝发生率差异无统计学意义。治疗组胎鼠平均体质量高于另3组,差异有统计学意义。维生素C组及治疗组的平均胎肺质量均高于对照组,对照组高于膈疝组,差异有统计学意义。膈疝组增生细胞比例低于另3组,差异有统计学意义。结论通过除草醚的应用,成功建立了大鼠的先天性膈疝模型。膈疝组胎鼠的肺脏质量降低,增生细胞比例减少,可能存在肺脏发育不良。维生素C不能明显改变胎鼠先天性膈疝的发生率,但可提高胎鼠肺脏质量,提高增生细胞的比例,促进胎肺发育。

关键词: 先天性膈疝, 肺脏发育不良, 除草醚, 维生素C, 大鼠

Abstract: Objective To induce a congenital diaphragmatic hernia model of fetal rat and to test the effect of vitamin C given in late gestation on the incidence of congenital diaphragmatic hernia(CDH) and lung development of fetal rat. Methods Sprague-Dawley female rats were randomly divided into four groups: control group(C), vitamin C group(W), CDH group(N) and treatment group(T). Nitrofen(100 mg) was given intragastrically to the rats of CDH and treatment groups on day 9.5 of gestation to induce a congenital diaphragmatic hernia model of fetal rat. Vitamin C(1.5 mg) was given intraperitoneally to vitamin C and treatment group from day 16.5 to 20.5. The offsprings were recovered on day 21(term=22nd). Fetal rats and their lungs were weighed. The incidence of CDH was examined. Immunohistochemical staining(Ki-67) was performed. The percentage of proliferative cells were counted by software. Results The incidences of CDH in CDH group and treatment group were the same. The mean body weight of treatment group was higher than those of the other three groups. The mean fetal lung weight was the highest in vitamin C group and treatment group and the lowest in CDH group. Percentage of proliferation cells in CDH group was lower than those of the other three groups. Conclusion By the use of nitrofen we induced congenital diaphragmatic hernia model in fetal rat. In the CDH group, the mean lung weight and the percentage of proliferation cells decreased. There may be lung hypoplasia in this group. There was no obvious evidence that vitamin C could change the incidence of CDH in rat model induced by nitrofen. Vitamin C increased mean lung weight and percentage of proliferation cells of CDH fetal rats, so it may improve lung development of fetal rats.

Key words: congenital diaphragmatic hernia, lung hypoplasia, nitrofen, vitamin C, rat

中图分类号:

R 321

蔡晶晶;陈永卫;张建敏. 妊娠晚期维生素C干预对大鼠先天性膈疝模型中胎肺的作用[J]. 首都医科大学学报, 2012, 33(2): 238-241.

CAI Jing-jing;CHEN Yong-wei;ZHANG Jian-min. The effect of vitamin C given in late gestation on the lung of fetal rat with congenital diaphragmatic hernia[J]. Journal of Capital Medical University, 2012, 33(2): 238-241.

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妊娠晚期维生素C干预对大鼠先天性膈疝模型中胎肺的作用

The effect of vitamin C given in late gestation on the lung of fetal rat with congenital diaphragmatic hernia

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