帕金森大鼠模型十二指肠黏膜β肾上腺素能受体不同亚型的表达变化

doi:10.3969/j.issn.1006-7795.2014.04.016

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (4): 463-467.doi: 10.3969/j.issn.1006-7795.2014.04.016

帕金森大鼠模型十二指肠黏膜β肾上腺素能受体不同亚型的表达变化

冯小燕, 樊瑞芳, 孙路, 张悦, 付小锁, 朱进霞

首都医科大学基础医学院生理与病理生理学系, 北京 100069

收稿日期:2014-01-09 出版日期:2014-08-21 发布日期:2014-07-22
通讯作者: 付小锁 E-mail:fxs_news@163.com
基金资助:
国家自然科学基金（81170346），北京市自然科学基金（7121003，7132017），首都医科大学基础临床科研合作基金。

Alterations in the expression of β-adrenoceptor subtypes in duodenal mucosa of Parkinson’s rats

Feng Xiaoyan, Fan Ruifang, Sun Lu, Zhang Yue, Fu Xiaosuo, Zhu Jinxia

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2014-01-09 Online:2014-08-21 Published:2014-07-22
Supported by:
This study was supported by National Natural Science Foundation of China (81170346), Beijing Natural Science Foundation(7121003, 7132017), Basic and Clinical Research Cooperation Fund of Capital Medical University.

摘要/Abstract

摘要：

目的研究β肾上腺素能受体不同亚型在6-羟多巴（6-hydroxydopamine，6-OHDA）制备的帕金森病（Parkinson's disease，PD）大鼠模型十二指肠黏膜的表达变化。方法通过双侧中枢黑质注射6-OHDA损毁多巴胺能神经元，建立6-OHDA大鼠模型。采用免疫荧光组织化学法和实时定量PCR技术检测β受体不同亚型（β₁、β₂、β₃）在6-OHDA大鼠模型十二指肠黏膜的表达变化。结果 β受体的3种亚型（β₁、β₂、β₃）在大鼠十二指肠黏膜均有表达，并且在6-OHDA大鼠模型的表达均上调，其中β₂表达量较β₁和β₃上调明显。结论 6-OHDA大鼠模型十二指肠黏膜β受体3种亚型的表达均上调，可能参与帕金森病胃肠功能紊乱的调节。

关键词: 6-羟多巴大鼠模型, 十二指肠, β受体

Abstract:

Objective To investigate the alterations of β-adrenoceptor subtypes in the duodenal mucosa of 6-hydroxydopamine(6-OHDA)-induced Parkinson's disease(PD) rat models. Methods Rats were subjected with 6-OHDA in bilateral substantia nigra(SN) to make the PD rat models. The location and quantitative detecting techniques including immunohistochemistry and real-time PCR were used to determine the alterations of β-adrenoceptor subtypes(β₁, β₂, β₃) in the PD rats. Results All three subtypes of β-adrenoceptor were found to express in the epithelial layer of duodenum in the model and control rats. However, the levels of the three receptors were higher in model rats than those of control rats with the β₂ subtype being most prominent. Conclusion The β-adrenoceptors(β₁, β₂, β₃) are up-regulated in the duodenal mucosa of 6-OHDA-induced PD rats, suggesting a potential mechanism for the gastrointestinal dysfunction in PD.

Key words: 6-hydroxydopamine(6-OHDA)-induced Parkinson’s disease rats, duodenum, β-adrenoceptor

中图分类号:

R592

冯小燕, 樊瑞芳, 孙路, 张悦, 付小锁, 朱进霞. 帕金森大鼠模型十二指肠黏膜β肾上腺素能受体不同亚型的表达变化[J]. 首都医科大学学报, 2014, 35(4): 463-467.

Feng Xiaoyan, Fan Ruifang, Sun Lu, Zhang Yue, Fu Xiaosuo, Zhu Jinxia. Alterations in the expression of β-adrenoceptor subtypes in duodenal mucosa of Parkinson’s rats[J]. Journal of Capital Medical University, 2014, 35(4): 463-467.

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帕金森大鼠模型十二指肠黏膜β肾上腺素能受体不同亚型的表达变化

Alterations in the expression of β-adrenoceptor subtypes in duodenal mucosa of Parkinson’s rats

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