脑室内注射脂多糖大鼠海马部位星形胶质细胞的变化

doi:10.3969/j.issn.1006-7795.2012.06.013

首都医科大学学报 ›› 2012, Vol. 33 ›› Issue (6): 771-775.doi: 10.3969/j.issn.1006-7795.2012.06.013

• 神经系统疾病的基础与临床研究 • 上一篇下一篇

脑室内注射脂多糖大鼠海马部位星形胶质细胞的变化

赵咏梅¹, 吕风月¹, 徐群渊²

1. 首都医科大学宣武医院神经变性病教育部重点实验室北京市老年病医疗研究中心, 北京 100053;2. 首都医科大学北京神经科学研究所, 北京 100069

收稿日期:2012-08-02 修回日期:1900-01-01 出版日期:2012-12-21 发布日期:2012-12-21
通讯作者: 赵咏梅

Changes of astrocytes in the hippocampus of a model of intra-ventricular injection of lipopolysaccharide in the rat

ZHAO Yongmei¹, LÜ Fengyue¹, XU Qunyuan²²

1. Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Xuanwu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Beijing 100053, China;2. Beijing Institute for Neuroscience, Capital Medical University, Beijing 100069, China

Received:2012-08-02 Revised:1900-01-01 Online:2012-12-21 Published:2012-12-21

摘要/Abstract

摘要： 目的研究侧脑室注射脂多糖(lipopolysaccharide,LPS)引发大鼠脑内炎性反应时,海马部位星形胶质细胞的变化。方法健康雄性SD大鼠64只,采用数字表法随机分为0.9%氯化钠注射液(normal saline,NS)对照组和LPS实验组。LPS实验组大鼠右侧脑室一次性注射LPS 20 μL(1.25 g/L),NS对照组大鼠脑室内注射同等体积的NS。于注射后2、4、12及24周应用免疫组织化学染色方法观察大鼠海马部位星形胶质细胞特异性标志物-胶质原纤维酸性蛋白(glial fibrillary acidic protein,GFAP)阳性细胞的变化,并应用Western blotting方法检测大鼠海马部位GFAP蛋白表达的变化。结果 GFAP免疫组织化学染色结果显示,NS对照组大鼠不同时间点海马部位星形胶质细胞均未见明显激活。LPS实验组大鼠海马部位星形胶质细胞在LPS注射后2周明显激活,而LPS注射后4周和12周时海马部位星形胶质细胞激活不明显。LPS注射后24周时,LPS实验组大鼠海马部位星形胶质细胞又出现轻度激活。Western blotting结果显示,NS对照组大鼠不同时间点海马部位GFAP蛋白表达量无明显变化。LPS实验组大鼠海马部位GFAP蛋白表达量在LPS注射后2周时明显高于相应NS对照组,为相应NS对照组的2.39倍,差异有统计学意义(P<0.01)。而注射后4周及12周时,LPS实验组大鼠海马部位GFAP蛋白表达量与相应NS对照组相比变化不明显。到注射后24周时,LPS实验组大鼠海马部位GFAP蛋白表达量为相应NS对照组的1.19倍,差异无统计学意义(P>0.05)。结论侧脑室注射LPS可在早期(注射后2周)显著引起大鼠海马部位星形胶质细胞的急性激活。

关键词: 炎性反应, 多巴胺能神经元, 海马, 星形胶质细胞

Abstract: Objective To investigate the change of astrocytes in the hippocampus in intracephalic inflammation rat model induced by intra-ventricular injection of lipopolysaccharide(LPS). Methods Altogether 64 healthy male SD rats were assigned into normal saline(NS)-injected group or LPS-injected group randomly. All injections were made intra-ventricularly on right side of the rats with NS 20 μL or LPS 20 μL(1.25 g/L). The changes of the the astrocytes in rat hippocampus were observed by immunohistochemical staining using astrocyte-specific marker-glial fibrillary acidic protein(GFAP) at 2 weeks, 4 weeks, 12 weeks and 24 weeks after NS or LPS injection. Western blot was performed to detect GFAP expression in the hippocampus of the rats. Results Immunohistochemical staining with GFAP antibody showed that the astrocytes in the hippocampus of rats were not activated at any time point after NS injection in NS-injected group. The astrocytes were activated obviously in the hippocampus of rats in LPS-injected group at 2 weeks after LPS injection. The activation of astrocytes was not found in the hippocampus in LPS-injected group at 4 and 12 weeks while the astrocytes were re-activated slightly in the hippocampus of LPS-injected group rats at 24 weeks after LPS injection. The result of Western blotting showed that the expression of GFAP in the hippocampus of NS-injected group rats did not change significantly at any time point after NS injection. The GFAP protein level in the hippocampus of LPS-injected group rats was 239% of that of NS-injected group rats at 2 weeks after injection(P<0.01). At 4 and 12 weeks after injection, the GFAP protein level in LPS-injected group was similar to that of NS-injected group. The expression of GFAP protein in the hippocampus of LPS-injected group was 119% of that of NS-injected group at 24 weeks post injection with no significant difference(P>0.05). Conclusion Intra-ventricular injection of LPS may result in acute and significant activation of astrocytes in the hippocampus of rats at early time(2 weeks post injection).

Key words: inflammation, dopaminergic neurons, hippocampus, astrocyte

中图分类号:

R592

赵咏梅;吕风月;徐群渊. 脑室内注射脂多糖大鼠海马部位星形胶质细胞的变化[J]. 首都医科大学学报, 2012, 33(6): 771-775.

ZHAO Yongmei;LÜFengyue;XU Qunyuan. Changes of astrocytes in the hippocampus of a model of intra-ventricular injection of lipopolysaccharide in the rat[J]. Journal of Capital Medical University, 2012, 33(6): 771-775.

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脑室内注射脂多糖大鼠海马部位星形胶质细胞的变化

Changes of astrocytes in the hippocampus of a model of intra-ventricular injection of lipopolysaccharide in the rat

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