大鼠局灶性脑缺血后神经功能评分与脑梗死体积相关性分析

doi:10.3969/j.issn.1006-7795.2013.01.013

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (1): 69-74.doi: 10.3969/j.issn.1006-7795.2013.01.013

• 神经系统疾病的基础研究 • 上一篇下一篇

大鼠局灶性脑缺血后神经功能评分与脑梗死体积相关性分析

王荣亮, 刘小蒙, 张营, 吉训明, 罗玉敏

首都医科大学宣武医院脑血管病研究室北京市老年病医疗研究中心, 北京 100053

收稿日期:2012-10-09 出版日期:2013-02-21 发布日期:2013-02-25
通讯作者: 罗玉敏 E-mail:yumin111@ccmu.edu.cn
基金资助:
国家自然科学基金面上项目(81071058, 30770743, 81271461)。

Correlation between neurological deficits scores and infarct volume in focal cerebral ischemia in rats

WANG Rongliang, LIU Xiaomeng, ZHANG Ying, JI Xunming, LUO Yumin

Cerebrovascular Diseases Research Institute, Xuanwu Hospital, Capital Medical University, Beijing Geriatric medical Research Center, Beijing 100053, China

Received:2012-10-09 Online:2013-02-21 Published:2013-02-25
Supported by:
This study was supported by National Science Foundation of China (81071058, 30770743, 81271461).

摘要/Abstract

摘要：

目的探讨大鼠大脑中动脉阻塞模型(middle cerebral artery occlusion, MCAO)的神经功能评分与脑梗死体积的相关性。方法 20只雄性Sprague-Dawley(SD)大鼠按随机数字表法分为5组,采用线栓法制作短暂性MCAO模型,于缺血再灌注后24 h、3 d、7 d、14 d、30 d进行三种神经功能评分(Longa 5分法、前肢踩空实验、Ludmila Belayev 12评分法),取脑组织后行HE染色。计算各组大鼠脑梗死体积相对百分比,采用方差分析及相关性分析统计结果。结果缺血再灌注后24 h到30 d,三种神经功能评分均有降低,7 d组与30 d组的前肢踩空实验和Ludmila Belayev 12评分法的评分结果相比较,差异有统计学意义(P<0.05)。脑梗死体积随着时间延长有所降低,但各组之间差异无统计学意义。三种神经功能评分中,缺血再灌注3 d组及30 d组,Ludmila Belayev 12分法评分的结果与脑梗死体积呈显著相关(P<0.05),其余时间点与三种神经功能缺失评分均无显著相关性。结论在线栓法制作的SD大鼠短暂性MCAO模型中,Ludmila Belayev 12评分法与缺血再灌注后第3 d、30 d的梗死体积有显著相关性,该评分法可用于评估这两个时间点的大鼠脑梗死损伤程度。

关键词: 脑梗死, 神经功能评分, 脑梗死体积

Abstract:

Objective To investigate the correlation between three scores of neurological deficits and infarct volume of middle cerebral artery occlusion (MCAO) model in rats. Methods A total of 20 Sprague-Dawley (SD) rats were randomly divided into five groups. MCAO models were induced. At five different time points (24 h, 3 d, 7 d, 14 d and 30 d after reperfusion), three grading methods (Longa test, Forelimb Placing test and Ludmila Belayev test) were used to evaluate neurological functions of rats. Then, the volume of infarction was calculated after taking the brain for HE staining. The correlations between neurological function scores and brain infarction volumes were analyzed by ANOVA and correlation.Results From 24 hours to 30 days after reperfusion, the scores of neurological deficits and the volume of infarction were decreased. There were significant differences in the scores of forelimb placing test and Ludmila Belayev test between 7 and 30 days after reperfusing (P<0.05). But the differences of infarction volume at different time points were insignificant (P>0.05). In terms of the correlation between the Ludmila Belayev score and infarction volume, there exists the obvious statistical significance at 3 (P<0.01) and 30 days (P<0.05). Conclusion For the MCAO model of rats, the Ludmila Belayev test can be used to evaluate the extent of injury after cerebral infarction at 3 and 30 days after reperfusion.

Key words: cerebral ischemia, neurological function scoring, infarct volume

中图分类号:

R284.2

王荣亮, 刘小蒙, 张营, 吉训明, 罗玉敏. 大鼠局灶性脑缺血后神经功能评分与脑梗死体积相关性分析[J]. 首都医科大学学报, 2013, 34(1): 69-74.

WANG Rongliang, LIU Xiaomeng, ZHANG Ying, JI Xunming, LUO Yumin. Correlation between neurological deficits scores and infarct volume in focal cerebral ischemia in rats[J]. Journal of Capital Medical University, 2013, 34(1): 69-74.

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大鼠局灶性脑缺血后神经功能评分与脑梗死体积相关性分析

Correlation between neurological deficits scores and infarct volume in focal cerebral ischemia in rats

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