己酮可可碱治疗非酒精性脂肪性肝炎的实验研究

doi:10.3785/j.issn.1006-7795.2009.03.013

首都医科大学学报 ›› 2009, Vol. 30 ›› Issue (3): 317-320.doi: 10.3785/j.issn.1006-7795.2009.03.013

己酮可可碱治疗非酒精性脂肪性肝炎的实验研究

崔焱, 张莉, 贾继东

首都医科大学附属北京友谊医院肝病中心

收稿日期:2009-02-28 修回日期:1900-01-01 出版日期:2009-06-21 发布日期:2009-06-21
通讯作者: 贾继东

A Trial of Pentoxifylline in Rat Nonalcoholic Steatohepatitis

CUI Yan, ZHANG Li, JIA Ji-dong

Liver Research Center, Beijing Friendship Hospital, Capital Medical University

Received:2009-02-28 Revised:1900-01-01 Online:2009-06-21 Published:2009-06-21

摘要/Abstract

摘要： 目的通过高脂饮食制备大鼠非酒精性脂肪肝模型,观察肝组织胶原的合成及相关细胞因子的变化。进一步观察及探讨己酮可可碱(pentoxifylline,PTX)对非酒精性脂肪性肝炎(nonalcoholic steatohepatitis,NASH)所致肝纤维化的治疗作用及机制。方法 30只 SD大鼠随机分为模型组(n=10)、治疗组(n=10)和正常对照组(n=10)3组。模型组和治疗组大鼠以高脂饲料喂养,治疗组大鼠高脂饮食12周后每d同时予以己酮可可碱16 mg/kg,及治疗4周。对照组大鼠以普通饲料饲养。实验16周处死3组大鼠。应用荧光定量PCR方法分别测定肝组织Ⅰ、Ⅲ型前胶原(procollagenⅠ、Ⅲ)、转化生长因子-β1(TGF-β1)及肿瘤坏死因子-α(TNF-α)的水平。结果大鼠肝组织中Ⅰ型前胶原mRNA的表达量模型组高于对照组,治疗组显著低于模型组,差异有统计学意义(P＜0.05);大鼠肝组织中Ⅲ型前胶原mRNA的表达量,模型组高于对照组,差异有统计学意义(P＜0.05),治疗组与模型组表达量差异无统计学意义;大鼠肝组织中TGF-β1mRNA的表达量模型组高于对照组,治疗组显著低于模型组,差异有统计学意义(P＜0.05);大鼠肝组织中TNF-α mRNA的表达量模型组高于对照组,治疗组显著低于模型组,差异有统计学意义(P＜0.05)。结论 TGF-β及TNF-α可能参与非酒精性脂肪肝肝纤维化的发生、发展过程。己酮可可碱可能通过抑制细胞因子TGF-β1和TNF-α降低脂肪性肝炎肝脏细胞外基质Ⅰ、Ⅲ型胶原的合成,对NASH引起的肝纤维化起到一定的治疗作用。

关键词: 非酒精性脂肪性肝炎, 己酮可可碱, 转化生长因子-β1, 肿瘤坏死因子-α

Abstract: Objective To investigate the effects and mechanism of pentoxifylline on nonalcoholic steatohepatitis. Methods Thirty SD rats were divided into 3 groups randomly: model group (n=10), treatment group(n=10), and normal control group(n=10). The rats of model group and treatment group were given fat-rich feed, and those of normal control group were given normal feed. Furthermore, the rats of treatment group were given pentoxifylline after 12 weeks of fat-rich diet feeding. At 16 weeks, the rats of treatment group, normal control group and model group were sacrificed. Livers were reserved to measure the procollagen Ⅰ, procollagen Ⅲ, TGF-β1 and TNF-α mRNA expression by real-time PCR. Results The model group's procollagenⅠmRNA expression level was higher than that of the normal control group(P＜0.05), that of the treatment group was lower than that of the model group(P＜0.05). Model group's procollagen Ⅲ mRNA expression level was higher than that of the normal control group(P＜0.05), that of the treatment group was lower than that of the model group, but the differences were no statistically significant. The model group's TGF-β1 mRNA expression level was higher than that of normal control group(P＜0.05), that of treatment group was lower than that of model group(P＜0.05). Model group's TNF-α mRNA expression level was higher than that of normal control group(P＜0.05), and that of treatment group was lower than that of model group(P＜0.05). Conclusion Pentoxifylline can decrease the collagen Ⅰand collagen Ⅲ mRNA expression of nonalcoholic steatohepatitis, inhibit TGF-β1 and TNF-α expression. Pentoxifylline may have certain anti-fibrotic effects.

Key words: nonalcoholic steatohepatitis, pentoxifylline, TGF-β1, TNF-α

中图分类号:

R 575.5

崔焱;张莉;贾继东. 己酮可可碱治疗非酒精性脂肪性肝炎的实验研究[J]. 首都医科大学学报, 2009, 30(3): 317-320.

CUI Yan;ZHANG Li;JIA Ji-dong. A Trial of Pentoxifylline in Rat Nonalcoholic Steatohepatitis[J]. Journal of Capital Medical University, 2009, 30(3): 317-320.

[1]	张辉, 马惠清, 王森茂, 王晓娟. 银杏叶提取物对帕金森病模型大鼠脑内神经炎性反应的影响[J]. 首都医科大学学报, 2019, 40(3): 439-442.
[2]	张金, 任思其, 李舒婷, 李苗, 龚小军, 孙艳玲, 武万水, 孙黎明, 杜淑旭. 脑脊液肿瘤坏死因子-α和干扰素-γ对髓母细胞瘤患儿的预后评价分析[J]. 首都医科大学学报, 2019, 40(2): 201-204.
[3]	矫健, 张罗. 转化生长因子-β1对慢性鼻-鼻窦炎伴鼻息肉上皮紧密连接屏障功能的影响[J]. 首都医科大学学报, 2018, 39(1): 45-50.
[4]	陈晨, 孟艳. 血管紧张素Ⅱ对树突状细胞功能的影响[J]. 首都医科大学学报, 2017, 38(5): 709-714.
[5]	王潇, 苏跃, 李天佐, 赵斌江. 胆碱能抗炎通路对大鼠脑缺血再灌注损伤的影响[J]. 首都医科大学学报, 2017, 38(3): 342-347.
[6]	颜繁诚, 王宁利. 青光眼视神经压力损伤相关分子机制研究进展[J]. 首都医科大学学报, 2017, 38(1): 38-41.
[7]	林梅, 张冬雪, 刘志强, 王左敏. 慢性牙周炎患者血清IL-33、TNF-α、IL-6的检测及意义[J]. 首都医科大学学报, 2016, 37(3): 255-259.
[8]	王芸姣, 韩文祺, 李若菲, 杜尊赎, 王学江, 江瑛. AMPK参与调节大鼠脂肪肝相关性肝癌前病变的形成[J]. 首都医科大学学报, 2016, 37(2): 208-213.
[9]	王沧海, 宿慧, 陶旭光, 林香春, 吴静, 台卫平, 刘揆亮. 铁道部机关体检人员非酒精性脂肪肝危险因素分析[J]. 首都医科大学学报, 2016, 37(1): 30-33.
[10]	穆珺;庄晓明;刘锐敏;曾静波. 血清巨噬细胞移动抑制因子、肿瘤坏死因子-α与糖尿病肾病的相关性研究[J]. 首都医科大学学报, 2012, 33(3): 385-388.
[11]	覃岭;王宇童. 过表达apoA-Ⅰ对BEL-7402细胞内脂质堆积的影响[J]. 首都医科大学学报, 2012, 33(2): 198-204.
[12]	陈阳育;张洪玉;庞宝森;李远红. 己酮可可碱可降低实验性阻塞性肺气肿大鼠炎性反应[J]. 首都医科大学学报, 2010, 31(5): 536-539.
[13]	武彦宁;蔡照华_;孙海梅;尚宏伟;郝刚;张立新;孙琳;张华;丁惠国. 非酒精性脂肪性肝炎大鼠肝脏内源性H₂S合成减少[J]. 首都医科大学学报, 2010, 31(3): 287-292.
[14]	安立新;彭宇明;王保国;孙梅珍;袁芳. 停通气预处理对脑出血大鼠炎性介质和氧化物的影响[J]. 首都医科大学学报, 2009, 30(4): 547-551.
[15]	段忠辉;李卓;于红卫;孟庆华. 慢性丙型肝炎病变程度与血清TNF-α水平、TNF-α 基因多态性的关系[J]. 首都医科大学学报, 2009, 30(3): 381-384.

己酮可可碱治疗非酒精性脂肪性肝炎的实验研究

A Trial of Pentoxifylline in Rat Nonalcoholic Steatohepatitis

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价