血管紧张素Ⅱ对树突状细胞功能的影响

doi:10.3969/j.issn.1006-7795.2017.05.016

首都医科大学学报 ›› 2017, Vol. 38 ›› Issue (5): 709-714.doi: 10.3969/j.issn.1006-7795.2017.05.016

血管紧张素Ⅱ对树突状细胞功能的影响

陈晨¹, 孟艳²

1. 首都医科大学附属北京潞河医院中美神经科学研究所, 北京 101100;
2. 首都医科大学基础医学院病理系, 北京 100069

收稿日期:2017-07-07 出版日期:2017-09-21 发布日期:2017-10-18
通讯作者: 孟艳 E-mail:littlemengyan@163.com

Ang Ⅱ activates dendritic cell to produce immune function

Chen Chen¹, Meng Yan²

1. China-America Institute of Neuroscience, Beijing Luhe Hospital, Capital Medical University, Beijing 101100, China;
2. Department of Pathology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2017-07-07 Online:2017-09-21 Published:2017-10-18

摘要/Abstract

摘要： 目的以肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）为对照，探讨血管紧张素Ⅱ（angiotensin Ⅱ，AngⅡ）对树突状细胞（dendritic cells，DC）的功能影响并比较DC对上述刺激的反应差异。方法体外培养DC2.4细胞，分别加入20 ng/mL TNF-α和100 nmol Ang Ⅱ诱导DC细胞成熟。用MTT、流式细胞仪、Transwell、混合淋巴细胞反应、FITC-Dxtra内吞实验、酶联免疫吸附试验等方法检测上述细胞的增生、分化成熟、迁徙、刺激淋巴细胞增生、内吞和分泌细胞因子白细胞介素-6（interleukin-6，IL-6）、干扰素-γ（interferon-γ，IFN-γ）等方面能力。结果与TNF-α相比，AngⅡ可以同等程度抑制DC的增生；可以诱导DC成熟标志表达，其能力弱于TNF-α；可以促进DC的迁徙，能力略弱于TNF-α；可以抑制树突状细胞的吞噬能力，其能力弱于TNF-α；促进相关细胞因子IL-6、IFN-γ分泌，与TNF-α作用基本一致；刺激淋巴细胞增生能力增强，其能力弱于TNF-α。结论 Ang Ⅱ具有和TNF-α相近的能力，对于树突状细胞的功能起着正向调节的作用。

关键词: 血管紧张素Ⅱ, 肿瘤坏死因子-α, 树突状细胞, 免疫

Abstract: Objective To investigate the immune effect of angiotensin Ⅱ(Ang Ⅱ) on immature dendritic cells (DC) compared with tumor necrosis factor-α (TNF-α)in vitro. Methods Prepared DC2.4 stimulated with different stimuli, such as 20 ng/mL TNF-α and 100 nmol Ang Ⅱ for indicated time. The effects of TNF-α and Ang Ⅱ on the proliferation, maturation, phagocytosis, cytokine secretion, migration, and communication with T cells of DCs are test by MTT, flow cytometry, ELISA, transwell assay and mixed lymphocyte culture respectively. Results First, the data showed that Ang Ⅱ stimulation significantly inhibited proliferation of DC, induced phenotypic maturation of DC, markedly increased the migration of DC, inhibited macrophage phagocytosis, promoted the secretion of interleukin-6 (IL-6) and interferon-γ(IFN-γ) and initiated the proliferation and activation of T-cell. Conclusion Compared with TNF-α, Ang Ⅱ positively modulated the function of DC to the same extent.

Key words: angiotensin Ⅱ (Ang Ⅱ), tumor necrosis factor-α, dendritic cell, immunity

中图分类号:

R392

陈晨, 孟艳. 血管紧张素Ⅱ对树突状细胞功能的影响[J]. 首都医科大学学报, 2017, 38(5): 709-714.

Chen Chen, Meng Yan. Ang Ⅱ activates dendritic cell to produce immune function[J]. Journal of Capital Medical University, 2017, 38(5): 709-714.

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血管紧张素Ⅱ对树突状细胞功能的影响

Ang Ⅱ activates dendritic cell to produce immune function

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