首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (6): 879-884.doi: 10.3969/j.issn.1006-7795.2013.06.019

• 基础研究 • 上一篇    下一篇

羟基红花黄色素A对脑缺血再灌注大鼠MMP-9和claudin-5蛋白水平的影响

戚智锋, 师文娟, 闫峰, 罗玉敏, 刘克建   

  1. 首都医科大学宣武医院脑血管病研究室, 北京 100053
  • 收稿日期:2013-07-16 出版日期:2013-12-21 发布日期:2013-12-13
  • 通讯作者: 刘克建 E-mail:kliu@salud.unm.edu
  • 基金资助:

    国家自然科学基金(81171242,81200928)。

Effects of hydroxysafflor yellow A on expression of MMP-9 and claudin-5 in cerebral ischemic/reperfusion rats

QI Zhifeng, SHI Wenjuan, YAN Feng, LUO Yumin, LIU Kejian   

  1. Cerebrovascular Diseases Research Institute, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
  • Received:2013-07-16 Online:2013-12-21 Published:2013-12-13
  • Supported by:

    This study was supported by the National Natural Science Foundation of China(81171242,81200928).

摘要:

目的 探讨羟基红花黄色素A(hydroxysafflor yellow A,HSYA)减轻大鼠脑缺血引起的血脑脊液屏障通透性增加的分子机制。方法 本研究利用大鼠脑缺血再灌注模型,研究HSYA对脑缺血后半影区和核心区基质金属蛋白酶-9(metalloproteinase-9,MMP-9)和紧密连接蛋白claudin-5水平的变化。结果 HSYA处理可以减少半影区MMP-9蛋白水平,而MMP-9的下游底物claudin-5的蛋白水平则显著增加。HSYA并不显著改变缺血核心区MMP-9和claudin-5蛋白水平。结论 本研究证明,活血化瘀中药HSYA可能通过减少半影区MMP-9蛋白水平,提高claudin-5的水平,从而减少半影区血脑脊液屏障损伤,为深入研究HSYA保护血脑脊液屏障的机制提供了参考。

关键词: 脑缺血, 羟基红花黄色素A, 基质金属蛋白酶-9, claudin-5, 血脑脊液屏障

Abstract:

Objective Hydroxysafflor yellow A(HSYA) is the water-extracted fraction from the flower of the safflower plant(C tinctorius), a traditional medicine, which has been used to treat ischemic diseases with a long history. Recent studies in rodents suggested that HSYA may reduce the blood brain barrier(BBB) permeability during acute cerebral ischemic/reperfusion. However, the mechanisms in BBB protection afforded by HSYA are still unclear. Methods In this study, we investigated the changes in protein level of matrix metalloproteinase-9(MMP-9) and tight junction protein claudin-5 in HSYA treatment on a 90 min-ischemia/72 h-reperfusion rat model, which mimics the possible clinical application for acute ischemic stroke patients. Results Western blotting indicated that HSYA reduced the MMP-9 level but increased the level of claudin-5 in penumbral tissue. However, there is no significant change in MMP-9 and claudin-5 level in ischemic core. Conclusion The reduction of MMP-9 and elevation of claudin-5 in penumbral might be involved in HSYA afforded BBB protection on ischemic/reperfusion model of rats.

Key words: cerebral ischemia, hydroxysafflor yellow A, metalloproteinase-9, claudin-5, blood brain barrier

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