热量限制对C57小鼠学习记忆能力及胰岛素信号通路相关蛋白的影响

doi:10.3969/j.issn.1006-7795.2014.06.011

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (6): 730-734.doi: 10.3969/j.issn.1006-7795.2014.06.011

• 神经系统常见病的基础研究 • 上一篇下一篇

热量限制对C57小鼠学习记忆能力及胰岛素信号通路相关蛋白的影响

赵志炜, 马丽娜, 王蓉, 张旭, 王玉兰, 吴燕川, 陈娟, 张景燕

首都医科大学宣武医院中心实验室北京市老年病医疗研究中心北京脑重大疾病研究院阿尔茨海默病研究所神经变性病教育部重点实验室, 北京 100053

收稿日期:2014-08-18 发布日期:2014-12-15
通讯作者: 王蓉 E-mail:rong_wang72@aliyun.com
基金资助:
国家自然科学基金(30772288),北京市自然科学基金(7132044);首都卫生发展科研专项项目(首发2011-1001-02)

Effects of caloric restriction on learning and memory function and insulin signaling related protein in C57 mice

Zhao Zhiwei, Ma Lina, Wang Rong, Zhang Xu, Wang Yulan, Wu Yanchuan, Chen Juan, Zhang Jingyan

Department of Central Laboratory, Xuanwu Hospital, Capital Medical University, Beijing Geriatric Medical Research Center, Center of Alzheimer's Disease, Beijing Institute for Brain Disorders, Key Laboratory for Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China

Received:2014-08-18 Published:2014-12-15
Supported by:
This study was supported by National Natural Science Foundation(30772288), Natural Science Foundation of Beijing(7132044), Capital Health Development Research(2011-1001-02).

摘要/Abstract

摘要：

目的探讨热量限制(caloric restriction,CR)对C57小鼠学习、记忆能力及胰岛素信号通路相关蛋白的影响。方法雄性C57小鼠30只,采用数字表法随机分为3组:正常对照组(n=10)、高能量组(n=10)、低能量组(n=10)。6个月后通过Morris水迷宫检测各组小鼠的学习、记忆能力,每组各抽取5只应用免疫组织化学染色的方法,测定小鼠脑内胰岛素信号转导通路相关蛋白:依赖于烟酰胺腺嘌呤二核苷酸的组蛋白脱乙酰酶(silent mating type information regulation 2 homolog- 1,SIRT1)、胰岛素样生长因子-1(insulin-like growth factor 1,IGF-1)、胰岛素受体(insulin receptor,IR)、胰岛素受体底物-1(insulin receptor substrate-1,IRS-1)、磷脂肌醇3激酶(phosphatidylinositol 3-kinase,PI3K)、蛋白激酶B(protein kinase B,Akt/PKB)、磷酸化应答原件结合蛋白(cAMP-response element binding protein,p-CREB)的表达。结果低能组小鼠的逃避潜伏期缩短,表明学习和记忆能力较前增高,SIRT1、IGF-1、IR、IRS-1、PI3K、Akt/PKB、p-CREB表达均明显降低(P<0.01)。结论热量饮食提高C57小鼠的学习记忆能力,可能与降低小鼠脑内胰岛素信号通路相关蛋白的表达有关。

关键词: 热量限制, 学习记忆, 胰岛素信号转导通路

Abstract:

Objective To investigate the effect of caloric restrictionon(CR) on learning and memory function and insulin signaling related protein in C57 mice. Methods Thirty male C57 mice were randomly divided into 3 groups: normal control group, high-energy group and low energy group, each group consisted of 10 mice, learning and memory function were measured by Morris test at the sixth month. Five mouse of each group were selected to measurethe insulin signaling pathway related protein by immunochemistry. Results The escape latency of low-energy group was shorter than normal control group, which indicates the learning and memory ability of low-energy C57 mice improved. The expression of Silent mating type information regulation 2 homolog- 1(SIRT1), insulin-like growth factor 1(IGF-1), insulin receptor(IR), insulin receptor substrate-1(IRS-1), phosphatidylinositol 3-kinase(PI3K), protein kinase B(Akt/PKB), cAMP-response element binding protein(p-CREB)in brain decreased significantly as compared with normal control group(P<0.01). Conclusion Caloric restriction can improve the learning and memory ability in C57 mice, probably through downregulate the expression of insulin signaling pathway related protein in brain.

Key words: caloric restriction, learning and memory ability, insulin signaling pathway

中图分类号:

赵志炜, 马丽娜, 王蓉, 张旭, 王玉兰, 吴燕川, 陈娟, 张景燕. 热量限制对C57小鼠学习记忆能力及胰岛素信号通路相关蛋白的影响[J]. 首都医科大学学报, 2014, 35(6): 730-734.

Zhao Zhiwei, Ma Lina, Wang Rong, Zhang Xu, Wang Yulan, Wu Yanchuan, Chen Juan, Zhang Jingyan. Effects of caloric restriction on learning and memory function and insulin signaling related protein in C57 mice[J]. Journal of Capital Medical University, 2014, 35(6): 730-734.

参考文献

[1] McCay C M, Crowell M F, Maynard L A. The effect of retarded growth upon the length of life span and upon the ultimate body size[J]. Nutrition,1989,5(3):155-171.
[2] Morris K C, Lin H W, Thompson J W, et al. Pathways for ischemic cytoprotection: role of sirtuins in caloric restriction, resveratrol, and ischemic preconditioning[J]. J Cereb Blood Flow Metab, 2011,31(4):1003-1019.
[3] 赵志炜,马丽娜,王蓉,等.热量限制对C57小鼠糖脂代谢的影响[J].中国比较医学,2013,23(8):20-24.
[4] Chouliaras L, van den Hove D L, Kenis G, et al. Prevention of age-related changes in hippocampal levels of 5-methylcytidine by caloric restriction[J]. Neurobiol Aging, 2012,33(8):1672-1681.
[5] De Lorenzo M S, Baljinnyam E, Vatner D E, et al. Caloric restriction reduces growth of mammary tumors and metastases[J]. Carcinogenesis, 2011,32(9):1381-1387.
[6] 赵静姝,王蓉.热量限制与神经保护作用[J].首都医科大学学报,2011,32(4):514-518.
[7] Gillette-Guyonnet S, Vellas B. Caloric restriction and brain function[J]. Curr Opin Clin Nutr Metab Care, 2008,11(3):686-692.
[8] D'Hooge R, De Deyn P. Applications of the Morris water maze in the study of learning and memory[J]. Brain Research Reviews, 2001,36(1):60-90.
[9] Datta S R, Dudek H, Xu T, et al. AKT phosphorylation of BAD couples survival signals to the cell-intrinsic death machinery[J]. Cell, 1997,91(2):231-241.
[10] Bond N, Everitt A V, Walton J. Effects of dietary restriction on radial-arm maze performance and flavor memory in aged rats[J]. Neurobiol Aging,1989,10(1):27-30.
[11] Yanai S, Okaichi Y, Okaichi H. Long-term dietary restriction causes negative effects on cognitive functions in rats[J]. Neurobiol Aging, 2004,25(3):325-332.
[12] 崔常清.胰岛素抵抗的机制与临床研究进展[J].中国煤炭工业医学杂志,2012,15(7):1119-1121.
[13] 陈敏,庄晓明,窦京涛,等.胰岛素自身免疫综合征的临床特征比较分析[J].首都医科大学学报,2012,33(3):409-413.
[14] 崔黎黎,赵传胜.2型糖尿病患者胰岛素抵抗与合并周围神经病变的相关性分析[J].中国医科大学学报,2012,41(8):734-737.

热量限制对C57小鼠学习记忆能力及胰岛素信号通路相关蛋白的影响

Effects of caloric restriction on learning and memory function and insulin signaling related protein in C57 mice

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 9

编辑推荐

Metrics

本文评价

[1]	余辉艳, 池亚菲, 李红睿, 王轩, 周雪阳, 肖荣, 席元第. 大豆卵磷脂联合大豆异黄酮改善大鼠学习记忆损伤及脑血管保护作用[J]. 首都医科大学学报, 2021, 42(6): 993-999.
[2]	张静爽, 马丽娜, 王蓉, 张景燕, 吴燕川, 赵志炜, 王玉兰. EX527对热量限制保护SH-SY5Y细胞的影响[J]. 首都医科大学学报, 2015, 36(5): 705-708.
[3]	赵静姝;王蓉. 热量限制与神经保护作用[J]. 首都医科大学学报, 2011, 32(4): 514-518.
[4]	张婷;王晓民. 从生命科学领域解读《Science》2009年度十大突破[J]. 首都医科大学学报, 2010, 31(1): 136-141.
[5]	冯波;胡鹏;王蓉. 突触后致密区与突触可塑性[J]. 首都医科大学学报, 2010, 31(1): 84-87.
[6]	孟祥宏;赵志炜;姬志娟;张景燕;王蓉. APP5肽类似物P165及罗格列酮对Ⅲ型糖尿病大鼠学习记忆功能的影响[J]. 首都医科大学学报, 2010, 31(1): 65-68.
[7]	余焕玲;毕研霞;肖荣;麻微微;向丽;丁冰杰;何玲玲. β谷固醇对高脂饲料喂养小鼠空问学习记忆能力的影响[J]. 首都医科大学学报, 2008, 29(6): 724-727.
[8]	邵志敏;张炙萍;赵志炜;庄晓明;姬志娟;张景燕;盛树力;王蓉. APP5肽类似物P165对2型糖尿病大鼠学习记忆的影响[J]. 首都医科大学学报, 2008, 29(2): 162-166.
[9]	庄晓明;穆珺;李然;陈敏;王蓉;姬志娟;赵志炜;盛树力. APP17肽对糖尿病KK小鼠海马神经元胰岛素信号转导途径的影响[J]. 首都医科大学学报, 2007, 28(3): 273-277.