ATP对人鼻腔纤毛摆动频率的影响

首都医科大学学报 ›› 2009, Vol. 30 ›› Issue (1): 57-61.

ATP对人鼻腔纤毛摆动频率的影响

史凌改¹, 韩德民^1,2, 张罗^1,2

1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科;2. 北京市耳鼻咽喉科研究所,耳鼻咽喉头颈科学教育部重点实验室

收稿日期:2008-11-18 修回日期:1900-01-01 出版日期:2009-02-21 发布日期:2009-02-21
通讯作者: 张罗

Effect of ATP on Human Nasal Ciliary Beat Frequency

Shi Linggai¹, Han Demin^1,2, Zhang Luo^1,2

1. Department of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University;2. Beijing Institute of Otorhinolaryngology, Key Laboratory of Otolaryngology Head and Neck Surgery, Ministry of Education

Received:2008-11-18 Revised:1900-01-01 Online:2009-02-21 Published:2009-02-21

摘要/Abstract

摘要： 目的观察不同浓度三磷酸腺苷(adenosine triphosphate,ATP)对体外培养人鼻腔纤毛运动的影响。方法采用组织块培养法,应用高速数字化显微成像系统观察1 μmol/L、10 μmol/L和100 μmol/L ATP对人鼻腔纤毛摆动频率(ciliary beat frequency,CBF)的影响。结果 1 Hanks平衡盐溶液(Hanks balanced salts solution,HBSS)对CBF无明显影响。2 1 μmol/L ATP使CBF由(9.2±1.8)Hz升至(10.3±2.0)Hz(n=10)(P＜0.05)。10 μmol/L ATP使CBF由(9.5±1.7)Hz升至(11.5±2.0)Hz(n=10)(P＜0.05)。3 100 μmol/L ATP使CBF由(8.7±1.1)Hz升至(11.5±1.4)Hz(n=10)(P＜0.05)。结论 ATP可刺激体外培养人鼻腔纤毛摆动频率升高,在1~100 μmol/L范围内,ATP对CBF的刺激呈浓度依赖性。

关键词: 纤毛摆动频率, 人鼻纤毛上皮细胞, 三磷酸腺苷

Abstract: Objective To investigate the effects of ATP on human nasal cilia movement. Methods Ciliary beat frequency(CBF) of cultured human nasal epithelial cells was measured by high-speed digital microscopy in ATP solution of different concentrations. Results CBF of cultured nasal epithelial cells exposed to HBSS showed no significant changes in 3 minutes(P>0.05); However in 1, 10 and 100 μmol/L ATP solution, CBF increased significantly in 10 seconds and reached the apex, then it showed a sustained elevation in 3 minutes. In 1 μmol/L ATP solution, CBF increased significantly from(9.2±1.8)Hz to(10.3±2.0)Hz(n=10)(P＜0.05). Similarly in 10 μmol/L ATP and 100 μmol/L ATP solution, CBF increased significantly when compared with samples in HBSS. Conclusion When 1, 10 and 100 μmol/L ATP were added to the chamber, there were rapid increase in ciliary activity. In addition, there is concentration-dependent relation between CBF and ATP.

Key words: ciliary beat frequency, human nasal epithelial cell, ATP

中图分类号:

R 765.25

史凌改;韩德民;张罗;. ATP对人鼻腔纤毛摆动频率的影响[J]. 首都医科大学学报, 2009, 30(1): 57-61.

Shi Linggai;Han Demin;Zhang Luo;. Effect of ATP on Human Nasal Ciliary Beat Frequency[J]. Journal of Capital Medical University, 2009, 30(1): 57-61.

[1]	王鸿;矫健;金善哲;张罗. 小鼠鼻腔及气管黏膜上皮细胞的构成和纤毛反应性比较研究[J]. 首都医科大学学报, 2011, 32(1): 21-25.
[2]	矫健;张罗;. 纤毛研究进展[J]. 首都医科大学学报, 2009, 30(1): 70-75.