低氧预适应对急性脑梗死小鼠血清TNF-α、IL-1β和IL-6炎性反应因子的影响

doi:10.3969/j.issn.1006-7795.2016.01.017

首都医科大学学报 ›› 2016, Vol. 37 ›› Issue (1): 89-92.doi: 10.3969/j.issn.1006-7795.2016.01.017

低氧预适应对急性脑梗死小鼠血清TNF-α、IL-1β和IL-6炎性反应因子的影响

张巧云¹, 李思颉², 牛敬忠³, 邵国⁴, 吉训明², 张颜波³

1. 天津市东丽区万新街社区卫生服务中心, 天津 300000;
2. 首都医科大学宣武医院低氧医学研究所, 北京 100053;
3. 泰山医学院附属医院神经内科, 山东泰安 271000;
4. 包头医学院生物医学研究中心, 内蒙古包头 014060

收稿日期:2015-06-11 出版日期:2016-02-21 发布日期:2016-02-01
通讯作者: 张颜波 E-mail:bbnnbn@163.com
基金资助:
低氧预适应转化医学北京市重点实验室开放研究课题(2015DYSY02)。

Effect of hypoxic preconditioning on the serum levels of TNF-α,IL-1β and IL-6 in mice with acute cerebral infarction

Zhang Qiaoyun¹, Li Sijie², Niu Jingzhong³, Shao Guo⁴, Ji Xunming², Zhang Yanbo³

1. Wanxinjie Community Health Centre, Dongli District of Tianjin, Tianjin 300000, China;
2. Institute for Hypoxia Medicine, Xuanwu Hospital, Capital Medical University, Beijing 100069, China;
3. Department of Neurology, Affiliated Hospital to Taishan Medical University, Taian 271000, Shandong Province, China;
4. Biomedical Research Center, Baotou Medical College, Baotou 014060, Inner Mongulia Autonomous Region, China

Received:2015-06-11 Online:2016-02-21 Published:2016-02-01
Supported by:
This study was supported by Open Research Project of Beijing Key Laboratory for Hypoxic Preconditioning and Translational Medicine(2015DYSY02).

摘要/Abstract

摘要： 目的探讨低氧预适应对急性脑梗死小鼠保护作用和血清肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)、白介素-1β(interleukin-1 beta,IL-1β)和白介素-6(interleukin-6, IL-6)炎性反应因子的影响。方法采用数字表法随机分为正常对照组(normal,N)、假手术组(control,C)、急性脑梗死组(cerebral infarction,CI)和低氧预适应(hypoxic preconditioning,HP)+急性脑梗死组(HP+CI)4组,每组小鼠6只,共24只。复制低氧预适应和光化学急性脑梗死模型,行为学方法和ELISA法分别检测神经功能缺损评分和TNF-α、IL-1β和IL-6浓度。结果 N、C组无神经功能缺损症状;CI、HP+CI组均出现明显神经功能缺损症状,CI、HP+CI组与N、C组相比,神经功能评分明显降低,差异有统计学意义(P<0.05);HP+CI组与CI组相比,神经功能明显改善,差异有统计学意义(P<0.05)。CI、HP+CI组小鼠血清TNF-α、IL-1β和IL-6明显升高,与N、C组相比,差异有统计学意义(P<0.05);HP+CI组与CI组相比,TNF-α、IL-1β和IL-6明显下降,差异有统计学意义(P<0.05)。结论低氧预适应对急性脑梗死具有保护作用,可能与减少TNF-α、IL-1β和IL-6炎性反应因子释放,抑制急性脑梗死后炎性反应有关。

关键词: 低氧预适应, 急性脑梗死, 神经功能评分, 炎性反应因子

Abstract: Objective To investigate the effect of hypoxic preconditioning on the serum levels of tumor necrosis factor alpha(TNF-α), interleukin-1 beta(IL-1β) and interleukin-6 (IL-6) in rats with acute cerebral infarction.Methods Twenty-four rats were randomly divided into the four groups: normal control group(normal,N), sham operation group(control,C), acute cerebral infarction group(cerebral infarction,CI), hypoxic preconditioning(hypoxic preconditioning,HP) and acute cerebral infarction group(HP+CI), with 6 rats in each group. Models of hypoxic preconditioning and photochemical acute cerebral infarction were duplicated.Behavioral method and ELISA were used to respectively measure neurological deficit scores and the levels of TNF-α, IL-1β and IL-6.Results No symptom of neurological deficit is shown in group N and C,while it showed up in group CI and HP+CI. The neurological scores in group CI and HP+CI decreased more clearly than those in group N and C (P<0.05). The neurological scores in group HP+CI improved significantly than those in group CI (P<0.05). The serum levels of TNF-α, IL-1β and IL-6 in group CI and HP+CI increased significantly than those in group N and C (P<0.05). The serum levels of TNF-α, IL-1β and IL-6 in group HP+CI decreased more clearly than those in group CI (P<0.05).Conclusion Hypoxic preconditioning, can protect animals from acute cerebral infarction and the protection may related to inhibition of inflammatory reaction (subsequent to acute cerebral infarction) induced by the decrease in release of TNF-α, IL-1β and IL-6.

Key words: hypoxic preconditioning, acute cerebral infarction, neurological function scoring, inflammatory cytokine

中图分类号:

R743

张巧云, 李思颉, 牛敬忠, 邵国, 吉训明, 张颜波. 低氧预适应对急性脑梗死小鼠血清TNF-α、IL-1β和IL-6炎性反应因子的影响[J]. 首都医科大学学报, 2016, 37(1): 89-92.

Zhang Qiaoyun, Li Sijie, Niu Jingzhong, Shao Guo, Ji Xunming, Zhang Yanbo. Effect of hypoxic preconditioning on the serum levels of TNF-α,IL-1β and IL-6 in mice with acute cerebral infarction[J]. Journal of Capital Medical University, 2016, 37(1): 89-92.

参考文献

[1] Kotur-Stevuljevic J, Bogavac-Stanojevic N, Jelic-Ivanovic Z, et al. Oxidative stress and paraoxonase 1 status in acute ischemic stroke patients[J]. Atherosclerosis,2015, 241(1):192-198.
[2] Jiang Y, Li L, Liu B, et al. PPARγ upregulation induced by vagus nerve stimulation exerts anti-inflammatory effect in cerebral ischemia/reperfusion rats[J].Med Sci Monit,2015,21:268-275.
[3] Doll D N, Rellick S L, Barr T L, et al. Rapid mitochondrial dysfunction mediates TNF-alpha-induced neurotoxicity[J]. J Neurochem,2015,132(4):443-451.
[4] Liu H, Wei X, Kong L, et al. NOD2 is involved in the inflammatory response after cerebral ischemia-reperfusion injury and triggers NADPH oxidase 2-derived reactive oxygen species[J]. Int J Biol Sci,2015,11(5):525-535.
[5] Dziedzic T. Systemic inflammation as a therapeutic target in acute ischemic stroke[J]. Expert Rev Neurother,2015,15(5):523-531.
[6] 苏芳, 朱雨岚,郭安臣,等. 脑缺血预适应标志物的评估[J].中国卒中杂志,2015,10(2):147-152.
[7] Zhang Y B, Wang X, Meister E A,et al. The effects of CoCl₂ on HIF-1α protein under experimental conditions of autoprogressive hypoxia using mouse models[J].Int J Mol Sci,2014,15(6):10999-11012.
[8] Li S, Zhang Y, Shao G, et al. Hypoxic preconditioning stimulates angiogenesis in ischemic penumbra after acute cerebral infarction[J]. Neural Regen Res,2013,8(31):2895-2903.
[9] Shao G, Lu G W. Hypoxic preconditioning in an autohypoxic animal model[J]. Neurosci Bull,2012,28(3):316-320.
[10] 侯伟健, 佟浩,柏树令,等. 动物低氧实验箱自动控制系统的组建及其在缺氧缺血脑损伤动物模型制作中的应用[J]. 中国医科大学学报,2013,42(2):138-142.
[11] 韩松, 张楠,尹艳玲,等. 蛋白激酶Cγ在低氧预适应脑保护中的作用及其缺血性卒中鼠脑半影区内相互作用蛋白鉴定[J]. 首都医科大学学报,2013,34(5):698-703.
[12] 胡瑞, 张晓云,晏雪,等. 脑出血后炎性反应机制的研究进展[J]. 中国脑血管病杂志,2015,12(7):370-374.
[13] 王鹏成, 任长虹,季泰令,等. 缺血性卒中后血管新生的研究进展[J]. 中国脑血管病杂志,2014,11(6):325-328.
[14] 刘云, 高晓莹,戚思华. 缺血后处理脑保护作用的研究进展[J]. 中国脑血管病杂志,2015,12(3):160-164.
[15] Tremblay M, Stevens B, Sierra A, et al. The role of microglia in the healthy brain[J]. J Neurosci,2011,31(45):16064-16069.
[16] Wake H, Moorhouse A J, Nabekura J. Functions of microglia in the central nervous system-beyond the immune response[J]. Neuron Glia Biol,2011,7(1):47-53.
[17] Street M E, Miraki-Moud F, Sanderson I R, et al. Interleukin-1beta (IL-1beta) and IL-6 modulate insulin-like growth factor-binding protein (IGFBP) secretion in colon cancer epithelial (Caco-2) cells[J]. J Endocrinol,2003,179(3):405-415.

低氧预适应对急性脑梗死小鼠血清TNF-α、IL-1β和IL-6炎性反应因子的影响

Effect of hypoxic preconditioning on the serum levels of TNF-α,IL-1β and IL-6 in mice with acute cerebral infarction

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	张心红, 杨楚琪, 王轩, 沈霞光, 房纯, 王凤英. 白细胞介素-6和超敏C反应蛋白与围产期颅内静脉窦血栓的相关性研究[J]. 首都医科大学学报, 2021, 42(2): 210-213.
[2]	王翼飞, 麻微微, 张淑华, 何电, 余焕玲, 肖荣. 三种膳食油脂对人血清补体、细胞炎性反应因子和趋化因子及其相关蛋白水平的调节作用及机制初探[J]. 首都医科大学学报, 2018, 39(2): 252-257.
[3]	张营, 刘涛, 于荣, 周明, 闫峰, 罗玉敏. 脑血疏对大鼠脑出血的行为学及凝血功能的影响[J]. 首都医科大学学报, 2017, 38(6): 841-845.
[4]	常佳慧, 赵晖, 王蕾, 张弛, 陆跃, 张秋霞. 侯氏黑散化学成分对脂多糖诱导BV2细胞活化中炎性反应因子分泌平衡的影响[J]. 首都医科大学学报, 2017, 38(2): 213-219.
[5]	刘思维, 贾伟华, 周立春. 急性脑梗死后非溶栓患者出血转化的影响因素分析[J]. 首都医科大学学报, 2016, 37(4): 534-537.
[6]	张媛媛, 李伟阳, 杨琳, 李丽英. 磷酸鞘胺醇调节小鼠单核巨噬细胞炎性细胞因子表达的机制研究[J]. 首都医科大学学报, 2015, 36(5): 729-733.
[7]	付璐, 王朝辉. CD62p在诊断急性脑梗死疾病中的作用[J]. 首都医科大学学报, 2015, 36(5): 714-717.
[8]	张营, 赵海苹, 吉训明, 高素琴, 罗玉敏. 远隔缺血后适应对脑缺血/再灌注损伤保护作用的研究[J]. 首都医科大学学报, 2013, 34(6): 844-850.
[9]	韩松, 张楠, 尹艳玲, 赵丽, 罗艳琳, 李俊发. 蛋白激酶Cγ在低氧预适应脑保护中的作用及其缺血性卒中鼠脑半影区内相互作用蛋白鉴定[J]. 首都医科大学学报, 2013, 34(5): 698-703.
[10]	何秀娟, 林燕, 吕喆, 安云庆, 丁跃中, 李萍. 朱红膏对脂多糖刺激人单核细胞系THP-1细胞分泌细胞因子和黏附分子的影响[J]. 首都医科大学学报, 2013, 34(4): 572-575.
[11]	王荣亮, 刘小蒙, 张营, 吉训明, 罗玉敏. 大鼠局灶性脑缺血后神经功能评分与脑梗死体积相关性分析[J]. 首都医科大学学报, 2013, 34(1): 69-74.
[12]	吴修信;李立新;廖品君;苑媛. 急性脑梗死患者血清淀粉样蛋白A的临床和预后价值[J]. 首都医科大学学报, 2012, 33(2): 255-258.
[13]	于培兰;隋雷鸣;韩松;赵焕英;江君;李俊发. 小鼠大脑中动脉栓塞脑局部缺血模型及其评价方法[J]. 首都医科大学学报, 2010, 31(5): 586-590.
[14]	董恺;刘萍;徐敏;马青峰;高利. 脑梗死患者舌象不对称性的临床观察[J]. 首都医科大学学报, 2010, 31(2): 174-176.
[15]	马青峰;贾建平;薛素芳;黄小钦;于跃怡;卢洁;张苗. 急性脑梗死患者血管异常与CT灌注成像及临床预后关系的研究[J]. 首都医科大学学报, 2010, 31(2): 149-153.