大黄提取液促进大鼠结肠肥大细胞和嗜酸性粒细胞增多反应

doi:10.3969/j.issn.1006-7795.2016.03.019

首都医科大学学报 ›› 2016, Vol. 37 ›› Issue (3): 348-351.doi: 10.3969/j.issn.1006-7795.2016.03.019

大黄提取液促进大鼠结肠肥大细胞和嗜酸性粒细胞增多反应

吴迪, 徐敬东, 朱进霞

首都医科大学基础医学院生理学与病理生理学系, 北京 100069

收稿日期:2016-03-01 出版日期:2016-06-21 发布日期:2016-06-13
通讯作者: 徐敬东, 朱进霞 E-mail:xujingdong@163.com;zhu_jx@ccmu.edu.cn
基金资助:
国家自然科学基金(81274173),北京市自然科学基金(7122017)。

Rhubarb extract solution enhances mast cells and eosinophils in rat colon

Wu Di, Xu Jingdong, Zhu Jinxia

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2016-03-01 Online:2016-06-21 Published:2016-06-13
Supported by:
This study was supported by National Natural Science Foundation of China (81274173), Natural Science Foundation of Beijing (7122017).

摘要/Abstract

摘要： 目的大黄具有清热解毒和润肠通便的作用。本实验结果显示短期服用大黄后,肥大细胞和嗜酸性粒细胞在大鼠的结肠有大量聚集。本研究意在探讨大黄提取液促进大鼠结肠肥大细胞和嗜酸性粒细胞增多反应的作用。方法使用免疫组织化学、免疫荧光及酶联免疫吸附的方法探讨不同剂量大黄灌胃后,大鼠结肠部位肥大细胞、嗜酸性粒细胞和干扰素-γ(interferon-γ,IFN-γ)及白细胞介素-10(interleukin-10,IL-10)的变化。结果大黄可激活肥大细胞、并可使嗜酸性粒细胞出现明显聚集,同时引起IFN-γ增加和IL-10降低的免疫保护机制。结论短期服用大黄可以引起结肠部位肥大细胞和嗜酸性粒细胞聚集,该作用对大黄的润肠通便作用具有重要意义。

关键词: 大黄, 肥大细胞, 嗜酸性粒细胞, 过敏反应

Abstract: Objective Since its effect of detoxification and laxative, it was found that mast cells and eosinophils have a large concentration on the rat colon after a short-term administration of Rhubarb in our investigation. This phenomenon of allergy caused by Rhubarb has not been reported. The aim of the study is to investigate the function that Rhubarb extract solution should be able to aggrandize mast cells and eosinophils on rat colon. Methods In order to explore the changes of mast cells, eosinophils, interferon-γ (IFN-γ) and interleukin-10 (IL-10) on the effect of different doses of Rhubarb, we gave the rats Rhubarb extract solution by intragastric administration. The samples were measured by means of immunohistochemistry, immunofluorescence and ELISA. Results Rhubarb could make mast cells activated and eosinophils accumulated significantly, causing an increase of IFN-γ and a decrease of IL-10, as while other phenomenon of allergy.Conclusion Administration of Rhubarb for 12 h can cause mast cell and eosinophil enhanced and accumulated in rat colon. It is vague how and why Rhubarb enhances the mast cell and eosinophil, which needs further research.

Key words: Rhubarb, mast cell, eosinophil, allergy

中图分类号:

R285

吴迪, 徐敬东, 朱进霞. 大黄提取液促进大鼠结肠肥大细胞和嗜酸性粒细胞增多反应[J]. 首都医科大学学报, 2016, 37(3): 348-351.

Wu Di, Xu Jingdong, Zhu Jinxia. Rhubarb extract solution enhances mast cells and eosinophils in rat colon[J]. Journal of Capital Medical University, 2016, 37(3): 348-351.

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大黄提取液促进大鼠结肠肥大细胞和嗜酸性粒细胞增多反应

Rhubarb extract solution enhances mast cells and eosinophils in rat colon

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