高内涵分析及Western blotting法在蛋白质核质分布研究中的应用及比较研究

doi:10.3969/j.issn.1006-7795.2016.05.012

首都医科大学学报 ›› 2016, Vol. 37 ›› Issue (5): 616-620.doi: 10.3969/j.issn.1006-7795.2016.05.012

高内涵分析及Western blotting法在蛋白质核质分布研究中的应用及比较研究

纪晓方¹, 李丽英², 常娜²

1. 首都医科大学2011级基础医学专业, 北京 100069;
2. 首都医科大学基础医学院细胞生物学系肝脏保护与再生调节北京市重点实验室, 北京 100069

收稿日期:2016-03-01 出版日期:2016-10-21 发布日期:2016-10-19
通讯作者: 常娜 E-mail:changna@ccmu.edu.cn
基金资助:
国家自然科学基金（31301154），北京市属高等学校创新团队建设与教师职业发展计划项目（IDHT20150502）。

Comparison between different methods to detect the subcellular localization of proteins

Ji Xiaofang¹, Li Liying², Chang Na²

1. Grade 2011 in Department of Basic Medicine, Capital Medical University, Beijing 100069, China;
2. Department of Cell Biology, School of Basic Medical Sciences, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University, Beijing 100069, China

Received:2016-03-01 Online:2016-10-21 Published:2016-10-19
Supported by:
This study was supported by National Natural Science Foundation of China (31301154), Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality (IDHT20150502).

摘要/Abstract

摘要： 目的使用不同方法对蛋白质的核质分布进行检测并比较差异。方法细胞经免疫荧光染色后使用高内涵分析（high content analysis，HCA）对蛋白质的细胞质或细胞核总荧光强度进行分析；分别提取胞质及胞核蛋白，使用Western blotting法对核质组分中的蛋白质含量进行鉴定。结果两种方法均可检测到蛋白质的出核转运；经定量分析，HCA法检测到的HuR蛋白质/核比升高倍数小于Western blotting法。结论使用HCA法检测蛋白质核质定位具有准确、客观、成本低、快捷、多参数等特点，不失为Western blotting的有效补充及替代方法。

关键词: 高内涵分析, Western blotting, 蛋白质核质分布

Abstract: Objective To compare different methods for detecting the subcellular localization of proteins. Methods Using high content analysis to count the cytoplasmic or nuclear fluorescence intensity of proteins. Results Cytoplasmic or nuclear proteins were separated and detected by Western blotting. Conclusion The cytoplasmic translocation of protein was detected by these two different methods conclusion high content analysis is better than Western blotting for detecting the subcellular localization of proteins.

Key words: high content analysis, Western blotting, subcellular localization of protein

中图分类号:

纪晓方, 李丽英, 常娜. 高内涵分析及Western blotting法在蛋白质核质分布研究中的应用及比较研究[J]. 首都医科大学学报, 2016, 37(5): 616-620.

Ji Xiaofang, Li Liying, Chang Na. Comparison between different methods to detect the subcellular localization of proteins[J]. Journal of Capital Medical University, 2016, 37(5): 616-620.

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高内涵分析及Western blotting法在蛋白质核质分布研究中的应用及比较研究

Comparison between different methods to detect the subcellular localization of proteins

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