探究胶质纤维酸性蛋白在6-OHDA大鼠胃体中的表达变化

doi:10.3969/j.issn.1006-7795.2016.05.011

首都医科大学学报 ›› 2016, Vol. 37 ›› Issue (5): 611-615.doi: 10.3969/j.issn.1006-7795.2016.05.011

探究胶质纤维酸性蛋白在6-OHDA大鼠胃体中的表达变化

任沁展, 郑丽飞, 刘玮, 朱进霞

首都医科大学基础医学院生理学与病理生理学系, 北京 100069

收稿日期:2015-12-18 出版日期:2016-10-21 发布日期:2016-10-19
通讯作者: 郑丽飞 E-mail:13581582937@163.com
基金资助:
国家自然科学基金（81170346，81370482，31400991），北京市自然科学基金（7121003，7132017），北京市属高等学校创新团队建设与教师职业发展计划项目（IDHT20140514），首都医科大学基础临床科研合作课题（14JL14）。

The expression of glial fibrillary acidic protein in gastric corpus of 6-OHDA rats

Ren Qinzhan, Zheng Lifei, Liu Wei, Zhu Jinxia

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2015-12-18 Online:2016-10-21 Published:2016-10-19
Supported by:
This study was supported by National Natural Science Foundation of China (81170346, 81370482, 31400991), Natural Science Foundation of Beijing (7121003, 7132017), Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality (IDHT20140514) and Basic-Clinical Scientific Research Cooperation Project of Capital Medical University (14JL14).

摘要/Abstract

摘要： 目的研究胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）在6-羟多巴（6-hydroxydopamine，6-OHDA）大鼠胃体中的表达变化情况，探讨神经胶质细胞在帕金森病（Parkinson’s disease，PD）胃轻瘫中的作用机制。方法用6-OHDA损毁大鼠中枢黑质多巴胺能神经元，建立PD模型。采用免疫荧光法检测酪氨酸羟化酶（tyrosine hydroxylase，TH）免疫阳性神经元在黑质的分布，并用同样的方法检测GFAP和神经微丝蛋白（neurofilament，NF）在大鼠胃体肌层的分布情况，采用蛋白免疫印记法（Western blotting）检测GFAP在6-OHDA模型大鼠胃体的表达情况。结果 6-OHDA大鼠黑质内神经元大量丢失，并伴有TH免疫反应性明显降低；6-OHDA大鼠胃体肌层GFAP和NF免疫反应性下降；Western blotting结果显示6-OHDA模型大鼠胃体肌层GFAP蛋白下调。结论 6-OHDA模型大鼠胃部GFAP和NF表达降低，可能参与了胃轻瘫发病的过程，本研究可能为PD患者胃轻瘫防治提供新的思路。

关键词: 6-羟多巴大鼠, 胶质纤维酸性蛋白, 神经微丝蛋白

Abstract: Objective To study the glial fibrillary acidic protein (GFAP) expression in gastric corpus of 6-hydroxydopamine (6-OHDA) treated rats and explore the underlying mechanism of enteric glial cells in gastroparesis of Parkinson's disease (PD). Methods 6-OHDA PD rat model was made by microinjecting 6-OHDA into bilateral substantia nigra. Immunofluorescence was used to observe the distribution of tyrosine hydroxylase (TH) immunoreactive (IR) neurons in the substantia nigra and the expression of GFAP and neurofilament (NF) in gastric corpus of 6-OHDA rats. Western blotting was applied to detect the expression of GFAP and NF in gastric corpus of 6-OHDA rats. Results In rats treated with 6-OHDA, the number of TH-IR neurons in the substantia nigra was significantly reduced; Immunofluorescence results showed that both GFAP and NF expression were distinctly decreased in gastric corpus of 6-OHDA rats; Compared with the control rats, the protein level of GFAP was significantly decreased in gastric corpus of 6-OHDA rats by Western blotting. Conclusion The results suggest that decrease of NF and GFAP in gastric corpus may play a role in the course of gastroparesis of 6-OHDA rats, which may provide a new idea for prevention of gastroparesis in PD.

Key words: 6-hydroxydopamine rats, glial fibrillary acidic protein, neurofilament

中图分类号:

R363

任沁展, 郑丽飞, 刘玮, 朱进霞. 探究胶质纤维酸性蛋白在6-OHDA大鼠胃体中的表达变化[J]. 首都医科大学学报, 2016, 37(5): 611-615.

Ren Qinzhan, Zheng Lifei, Liu Wei, Zhu Jinxia. The expression of glial fibrillary acidic protein in gastric corpus of 6-OHDA rats[J]. Journal of Capital Medical University, 2016, 37(5): 611-615.

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探究胶质纤维酸性蛋白在6-OHDA大鼠胃体中的表达变化

The expression of glial fibrillary acidic protein in gastric corpus of 6-OHDA rats

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