Siewert Ⅱ型食管胃结合部腺癌组织中PDZK1和PDGFR-β的表达及相互作用

doi:10.3969/j.issn.1006-7795.2017.06.005

首都医科大学学报 ›› 2017, Vol. 38 ›› Issue (6): 804-809.doi: 10.3969/j.issn.1006-7795.2017.06.005

Siewert Ⅱ型食管胃结合部腺癌组织中PDZK1和PDGFR-β的表达及相互作用

蒲昌盛, 郑智, 蔡军, 唐华臻, 张军, 张忠涛

首都医科大学附属北京友谊医院普外科, 北京 100050

收稿日期:2017-10-16 出版日期:2017-11-21 发布日期:2017-12-16
通讯作者: 张军 E-mail:zhangjundoctor@163.com
基金资助:
首都特色临床研究资助项目（Z131107002213046），北京市卫生系统高层次卫生技术人才培养计划资助项目（2013-3-067）。

Expression and interaction of PDZK1 and PDGFR-β in adenocarcinoma of the esophagogastric junction of Siewert Ⅱ

Pu Changsheng, Zheng Zhi, Cai Jun, Tang Huazhen, Zhang Jun, Zhang Zhongtao

Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China

Received:2017-10-16 Online:2017-11-21 Published:2017-12-16
Supported by:
This study was supported by Capital Characteristic Clinical Research Project (Z131107002213046), Beijing Municipal Health System High-level Health Technician Training Program (2013-3-067).

摘要/Abstract

摘要： 目的探究Siewert Ⅱ型食管胃结合部腺癌组织中PDZ蛋白激酶1（PDZ kinase 1，PDZK1）和血小板源性生长因子受体-β（platelet-derived growth factor receptor-β，PDGFR-β）的表达以及相互之间的作用，对比PDZK1和PDGFR-β在Siewert Ⅱ型食管胃结合部腺癌与远端胃癌的表达差异，探寻PDZK1是否能成为食管胃结合部腺癌靶向治疗的新靶点和分子分型的依据。方法从患者组织蜡块中采集标本，制作成石蜡切片，进行免疫组织化学染色。采用半定量评分系统评定免疫组织化学染色结果并做统计分析。结果 PDZK1在食管胃结合部腺癌组织中阳性表达率为30%（12/40），在食管胃结合部正常组织中阳性表达率为95%（38/40），差异无统计学意义（P=0.268）。PDGFR-β在食管胃结合部腺癌组织中阳性表达率为80%（32/40），食管胃结合部正常组织中阳性表达率为28%（11/40），差异无统计学意义（P=0.648）。PDZK1和PDGFR-β在食管胃结合部正常组织无明显相关性（r=0.026，P=0.874），PDZK1和PDGFR-β在食管胃结合部腺癌组织无明显相关性（r=0.111，P=0.497）。对比本课题组前期研究的远端胃癌，结果显示PDZK1在胃食管结合部正常组织中表达要高于远端胃正常组织（Z=-1.997，P=0.046），同样PDGFR-β在胃食管结合部腺癌组织中表达要高于远端胃癌组织（Z=-3.811，P=0.000），差异具有统计学意义。结论 PDGFR-β在Siewert Ⅱ食管胃结合部腺癌组织中呈现高表达，PDZK1在Siewert Ⅱ食管胃结合部正常组织中呈现高表达。PDZK1与PDGFR-β在Siewert Ⅱ食管胃结合部腺癌组织和正常组织中的表达均未表现出相关性。Siewert Ⅱ型食管胃结合部腺癌中PDZK1与PDGFR-β的表达要高于远端胃癌，结合其不同于胃癌的临床特点，食管胃结合部腺癌有可能是不同于远端胃癌的一类特殊肿瘤，而且PDZK1有可能在食管胃结合部腺癌的分子分型和靶向治疗方面提供一定的参考价值，为后续的研究提供了新的思路。

关键词: 食管胃结合部腺癌, PDZ蛋白激酶l, 血小板源性生长因子受体-β, 胃癌

Abstract: Objective To study the expression and interaction of PDZ kinase 1 (PDZK1) and platelet-derived growth factor receptor-β (PDGFR-β) in Siewert Ⅱ adenocarcinoma of the esophagogastric junction. To compare the expression of PDZK1 and PDGFR-β in Siewert Ⅱ adenocarcinoma of the esophagogastric junction and distal gastric cancer. To investigate whether PDZK1 is a new target of adenocarcinoma of the esophagogastric junction (AEG) targeted therapy and the basis of molecular typing.Methods Paraffin sections were prepared for immunohistochemical staining. Semi-quantitative scoring system was used to evaluate the results of immunohistochemical staining and then statistical analysis was performed. Results There was 30% (12/40) positive rate of PDZK1 in the adenocarcinoma of the esophagogastric junction. There was 95% (38/40) positive rate of PDZK1 in the normal tissues of esophagogastric junction. There was no significant difference between two tissues (P=0.268). There was 80% (32/40) positive rate of PDGFR-β in the adenocarcinoma of the esophagogastric junction. There was 28% (11/40) positive rate of PDGFR-β in the normal tissue of esophagogastric junction. There was no significant difference between two tissues (P=0.648). There was no significant correlation between PDZK1 and PDGFR-β in the normal tissue of the esophagogastric junction (r=0.026, P=0.874) and the adenocarcinoma of the esophagogastric junction (r=0.111, P=0.497). Compared with distal gastric cancer, the expression of PDZK1 in the normal tissue of the esophagogastric junction was higher than that in the distal gastric tissues (Z=-1.997,P=0.046). the expression of PDGFR-β in the adenocarcinoma tissues of the gastroesophageal junction was higher than that in the distal gastric tissues (Z=-3.811,P=0.000), there was significant difference.Conclusion There was highly expression of PDGFR-β in adenocarcinoma of the esophagogastric junction. There was highly expression of PDZK1 in normal tissues of the esophagogastric junction. There was no significant correlation between PDZK1 and PDGFR-β in the normal tissues of the esophagogastric junction and the adenocarcinoma of the esophagogastric junction. Combined with the clinical characteristics, the adenocarcinoma of esophagogastric junction may be different from the distal gastric cancer. With the determination of expression of PDZK1, it is possible to provide some reference value in AEG molecular classification and targeted therapy.

Key words: adenocarcinoma of the esophagogastric junction, PDZ kinase 1(PDZK1), platelet-derived growth factor receptor-β(PDGFR-β), gastric cancer

中图分类号:

R656

蒲昌盛, 郑智, 蔡军, 唐华臻, 张军, 张忠涛. Siewert Ⅱ型食管胃结合部腺癌组织中PDZK1和PDGFR-β的表达及相互作用[J]. 首都医科大学学报, 2017, 38(6): 804-809.

Pu Changsheng, Zheng Zhi, Cai Jun, Tang Huazhen, Zhang Jun, Zhang Zhongtao. Expression and interaction of PDZK1 and PDGFR-β in adenocarcinoma of the esophagogastric junction of Siewert Ⅱ[J]. Journal of Capital Medical University, 2017, 38(6): 804-809.

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Siewert Ⅱ型食管胃结合部腺癌组织中PDZK1和PDGFR-β的表达及相互作用

Expression and interaction of PDZK1 and PDGFR-β in adenocarcinoma of the esophagogastric junction of Siewert Ⅱ

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