miR-29s通过下调大麻素受体1抑制ACEA促J774A.1细胞迁移的活性

doi:10.3969/j.issn.1006-7795.2018.02.015

首都医科大学学报 ›› 2018, Vol. 39 ›› Issue (2): 230-235.doi: 10.3969/j.issn.1006-7795.2018.02.015

miR-29s通过下调大麻素受体1抑制ACEA促J774A.1细胞迁移的活性

段向辉, 常娜, 李丽英

首都医科大学基础医学院细胞生物学系, ‘肝脏保护与再生调节’北京市重点实验室, 北京 100069

收稿日期:2017-08-28 出版日期:2018-03-21 发布日期:2018-04-14
通讯作者: 李丽英 E-mail:xin.ma@jefferson.edu
基金资助:
国家自然科学基金（81270283，81700270），代谢紊乱相关心脏疾病北京市重点实验室基金（PXM2015_014226_000062），首都医科大学燕京医学院学院基金（11723403）。

miR-29s inhibit ACEA-induced J774A.1 cell migration by down regulation CB1 expression

Duan Xianghui, Chang Na, Li Liying

Department of Cell Biology, School of Basic Medical Sciences, Capital Medical University, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Beijing 100069, China

Received:2017-08-28 Online:2018-03-21 Published:2018-04-14
Supported by:
This study was supported by National Natural Sciences Foundation of China(81270283, 81700270), Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases (PXM2015_014226_000062), Capital Medical University Yanjing Medical College Initial Fund(11723403).

摘要/Abstract

摘要： 目的研究miR-29a-3p、miR-29b-3p和miR-29c-3p是否可以影响J774A.1细胞中大麻素受体1（cannabinoid receptor type 1，CB1）的表达以及其迁移功能。方法 RT-qPCR法检测CB1 mRNA表达；Boyden chamber法检测J774A.1细胞迁移能力的变化。结果本研究证明了在J774A.1细胞里，CB1的激动剂ACEA（Arachidonyl-2'-chloroethylamide）可以上调CB1 mRNA的表达，而分别转染了miR-29a-3p、miR-29b-3p和miR-29c-3p的mimics后可以抑制这一过程；同时对J774A.1细胞转染miR-29a-3p、miR-29b-3p和miR-29c-3p的mimics可以阻碍ACEA诱导的J774A.1细胞的迁移。结论 miR-29s通过下调CB1的表达抑制了ACEA促J774A.1细胞迁移的活性。

关键词: miR-29, 大麻素受体1, 细胞迁移

Abstract: Objective To investigate whether miR-29a-3p, miR-29b-3p and miR-29c-3p affect cannabinoid receptor 1(CB1)expression and cell migration in J774A.1 cells. Methods RT-qPCR was used to detect CB1 mRNA expression in J774A.1 cells. Boyden chamber assay was used for the detection of cell migration. Results In this study, we presented evidences that CB1 agonist Arachidonyl-2'-chloroethylamide (ACEA) upregulates the expression of CB1 mRNA in J774A.1 cells. Whereas miR-29a-3p or miR-29b-3p or miR-29c-3p mimics blocks the increase of CB1 mRNA level and ACEA-induced cell migration in J774A.1 cells. Conclusion miR-29s inhibit ACEA-induced J774A.1 cell migration via downregulating CB1 expression.

Key words: miR-29, cannabinoid receptor type 1 (CB1), cell migration

中图分类号:

段向辉, 常娜, 李丽英. miR-29s通过下调大麻素受体1抑制ACEA促J774A.1细胞迁移的活性[J]. 首都医科大学学报, 2018, 39(2): 230-235.

Duan Xianghui, Chang Na, Li Liying. miR-29s inhibit ACEA-induced J774A.1 cell migration by down regulation CB1 expression[J]. Journal of Capital Medical University, 2018, 39(2): 230-235.

参考文献

[1] Wynn T A, Chawla A, Pollard J W. Macrophage biology in development, homeostasis and disease[J]. Nature, 2013, 496(7446):445-455.
[2] Mai P, Yang L, Tian L, et al. Endocannabinoid system contributes to liver injury and inflammation by activation of bone marrow-derived monocytes/macrophages in a CB1-Dependent manner[J]. J Immunol, 2015, 195(7):3390-3401.
[3] Zhou Y, Falenta K, Lalli G. Endocannabinoid signalling in neuronal migration[J]. Int J Biochem Cell Biol, 2014, 47:104-108.
[4] Saez T M, Aronne M P, Caltana L, et al. Prenatal exposure to the CB1 and CB2 cannabinoid receptor agonist WIN 55,212-2 alters migration of early-born glutamatergic neurons and GABAergic interneurons in the rat cerebral cortex[J]. J Neurochem, 2014, 129(4):637-648.
[5] Preet A, Qamri Z, Nasser M W, et al. Cannabinoid receptors, CB1 and CB2, as novel targets for inhibition of non-small cell lung cancer growth and metastasis[J]. Cancer Prev Res (Phila), 2011, 4(1):65-75.
[6] Nithipatikom K, Gomez-Granados A D, Tang A T, et al. Cannabinoid receptor type 1(CB1) activation inhibits small GTPase RhoA activity and regulates motility of prostate carcinoma cells[J]. Endocrinology, 2012, 153(1):29-41.
[7] Hammond S M. An overview of microRNAs[J]. Adv Drug Deliv Rev, 2015, 87:3-14.
[8] Mohr A M, Mott J L. Overview of microRNA biology[J]. Semin Liver Dis, 2015, 35(1):3-11.
[9] Wang B, Wu H, Chai C, et al. MicroRNA-1301 suppresses tumor cell migration and invasion by targeting the p53/UBE4B pathway in multiple human cancer cells[J]. Cancer Lett, 2017, 401:20-32.
[10] Sandbothe M, Buurman R, Reich N, et al. The microRNA-449 family inhibits TGF-beta-mediated liver cancer cell migration by targeting SOX4[J]. J Hepatol, 2017, 66(5):1012-1021.
[11] Patel V, Noureddine L. MicroRNAs and fibrosis[J]. Curr Opin Nephrol Hypertens, 2012, 21(4):410-416.
[12] Mallat A, Teixeira-Clerc F, Lotersztajn S. Cannabinoid signaling and liver therapeutics[J]. J Hepatol, 2013, 59(4):891-896.
[13] Jourdan T, Demizieux L, Gresti J, et al. Antagonism of peripheral hepatic cannabinoid receptor-1 improves liver lipid metabolism in mice:evidence from cultured explants[J]. Hepatology, 2012, 55(3):790-799.
[14] Giannone F A, Baldassarre M, Domenicali M, et al. Reversal of liver fibrosis by the antagonism of endocannabinoid CB1 receptor in a rat model of CCl(4)-induced advanced cirrhosis[J]. Lab Invest, 2012, 92(3):384-395.
[15] Gentilini D, Besana A, Vigano P, et al. Endocannabinoid system regulates migration of endometrial stromal cells via cannabinoid receptor 1 through the activation of PI3K and ERK1/2 pathways[J]. Fertil Steril, 2010, 93(8):2588-2593.
[16] Song Z H, Zhong M. CB1 cannabinoid receptor-mediated cell migration[J]. J Pharmacol Exp Ther, 2000, 294(1):204-209.
[17] Li Y, Zhang Z. Computational biology in microRNA[J]. Wiley Interdiscip Rev RNA, 2015, 6(4):435-452.
[18] Virant-Klun I, Stahlberg A, Kubista M, et al. MicroRNAs:from female fertility, germ cells, and stem cells to cancer in humans[J]. Stem Cells Int, 2016, 2016:3984937.
[19] Szabo G, Bala S. MicroRNAs in liver disease[J]. Nat Rev Gastroenterol Hepatol, 2013, 10(9):542-552.
[20] Jones-Rhoades M W, Bartel D P, Bartel B. MicroRNAS and their regulatory roles in plants[J]. Annu Rev Plant Biol, 2006, 57:19-53.
[21] Lewis B P, Burge C B, Bartel D P. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets[J]. Cell, 2005, 120(1):15-20.
[22] Matsumoto Y, Itami S, Kuroda M, et al. MiR-29a assists in preventing the activation of human stellate cells and promotes recovery from liver fibrosis in mice[J]. Mol Ther, 2016, 24(10):1848-1859.
[23] He Y, Huang C, Lin X, et al. MicroRNA-29 family, a crucial therapeutic target for fibrosis diseases[J]. Biochimie, 2013, 95(7):1355-1359.
[24] Geissmann F, Manz M G, Jung S, et al. Development of monocytes, macrophages, and dendritic cells[J]. Science, 2010, 327(5966):656-661.
[25] Sindrilaru A, Peters T, Wieschalka S, et al. An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice[J]. J Clin Invest, 2011, 121(3):985-997.
[26] Krausgruber T, Blazek K, Smallie T, et al. IRF5 promotes inflammatory macrophage polarization and TH1-TH17 responses[J]. Nat Immunol, 2011, 12(3):231-238.
[27] Moreira F A, Crippa J A. The psychiatric side-effects of rimonabant[J]. Rev Bras Psiquiatr, 2009, 31(2):145-153.
[28] 潘霞,王智慧,雷小勇.microRNAs与ABC转运蛋白介导的肿瘤多药耐药研究进展2015,22(8):644-648.
[29] 童春华,李新建,李志晋.微小RNA在肝癌组织中的表达及其潜在临床价值探讨[J].中华肿瘤防治杂志,2016,23(S1):28-29.

miR-29s通过下调大麻素受体1抑制ACEA促J774A.1细胞迁移的活性

miR-29s inhibit ACEA-induced J774A.1 cell migration by down regulation CB1 expression

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