血管紧张素Ⅱ1型受体自身抗体阳性雌鼠孕后出现妊娠期高血压样症状

doi:10.3969/j.issn.1006-7795.2019.02.019

首都医科大学学报 ›› 2019, Vol. 40 ›› Issue (2): 257-262.doi: 10.3969/j.issn.1006-7795.2019.02.019

血管紧张素Ⅱ1型受体自身抗体阳性雌鼠孕后出现妊娠期高血压样症状

边经纬, 尹晓辰, 李浩, 张苏丽, 王美丽, 刘慧荣

首都医科大学生理学与病理生理学系代谢紊乱相关心血管疾病北京市重点实验室代谢紊乱相关心血管疾病国际合作实验室, 北京 100069

收稿日期:2018-11-22 出版日期:2019-03-21 发布日期:2019-04-15
通讯作者: 刘慧荣 E-mail:liuhr2000@ccmu.edu.cn
基金资助:
国家自然科学基金（91539205）。

Angiotensin Ⅱ type 1 receptor autoantibody-positive female rats showed hypertensive disorder after pregnancy

Bian Jingwei, Yin Xiaochen, Li Hao, Zhang Suli, Wang Meili, Liu Huirong

Department of Physiology & Pathophysiology, Beijing Key Laboratory of Cardiovascular Diseases and Related Metabolic Dysfunction, Capital Medical University, International Cooperative Laboratory of Cardiovascular Diseases and Related Metabolic Dysfunction, Beijing 100069, China

Received:2018-11-22 Online:2019-03-21 Published:2019-04-15
Supported by:
This study was supported by National Natural Science Foundation of China (91539205).

摘要/Abstract

摘要： 目的探究血管紧张素Ⅱ1型受体自身抗体（angiotensin Ⅱ type 1 receptor autoantibody，AT1-AA）阳性大鼠孕后是否出现类妊娠期高血压疾病的表现。方法收集临床血清样品，通过酶联免疫吸附实验（enzyme linked immunosorbent assay，ELISA）检测非妊娠的健康女性体内是否含有AT1-AA。通过主动免疫方法建立AT1-AA阳性大鼠模型，利用尾套法测量大鼠收缩压，肠系膜动脉血管环检测血管功能。结果 8.3%未妊娠的健康女性体内含有AT1-AA。AT1-AA阳性大鼠怀孕前与对照组大鼠的血压比较，差异无统计学意义（P>0.05）；但怀孕后AT1-AA阳性大鼠的收缩压较对照组孕鼠显著升高。肠系膜动脉血管环实验结果显示，AT1-AA阳性大鼠怀孕后血管对收缩物质的反应性增强（对照组：110%±5%，模型组：130%±10%，P<0.05），且内皮依赖/非依赖血管舒张功能降低（对照组：90%±4%，模型组：60%±7%/对照组：95%±1%，模型组：75%±3%，P<0.05）。结论 AT1-AA阳性大鼠妊娠期间出现血压升高，阻力血管功能受损等类妊娠期高血压的病理表现，提示健康人体内存在的AT1-AA是妊娠期高血压疾病发生的潜在危险因素。

关键词: 血管紧张素Ⅱ1型受体, 自身抗体, 血压, 血管功能受损

Abstract: Objective To investigate whether angiotensin Ⅱ type 1 receptor autoantibody (AT1-AA)-positive rats exhibited the phenomenon of gestational hypertension after pregnancy. Methods Clinical serum samples were collected to detect the presence of AT1-AA in non-pregnant healthy women by enzyme linked immunosorbent assay (ELISA). AT1-AA-positive rat model was established by active immunization. Tail-cuff method was used to measure the systolic blood pressure and the mesenteric artery ring was used to detect vascular function. Results AT1-AA was also present in some healthy women who were not pregnant. There was no significant difference in blood pressure between AT1-AA-positive rats and control rats before pregnancy, but the systolic pressure of AT1-AA-positive rats after pregnancy was significantly higher than that of control rats. Mesenteric artery ring showed that the vasoconstriction was increased (vehicle group:110%±5%, immunized group:130%±10%, P<0.05), and endothelium-dependent/non-endothelium-dependent vasodilation was decreased in AT1-AA-positive rats after pregnancy (vehicle group:90%±4%, immunized group:60%±7%/vehicle group:95%±1%, immunized group:75%±3%, P<0.05). Conclusion AT1-AA-positive rats after pregnancy showed the phenomenon of gestational hypertension, such as elevated blood pressure and impaired vascular function. These suggested that the presence of AT1-AA in healthy individuals was a risk factor for gestational hypertension.

Key words: Angiotensin Ⅱ type 1 receptor, autoantibody, blood pressure, vascular dysfunction

中图分类号:

R714.252

边经纬, 尹晓辰, 李浩, 张苏丽, 王美丽, 刘慧荣. 血管紧张素Ⅱ1型受体自身抗体阳性雌鼠孕后出现妊娠期高血压样症状[J]. 首都医科大学学报, 2019, 40(2): 257-262.

Bian Jingwei, Yin Xiaochen, Li Hao, Zhang Suli, Wang Meili, Liu Huirong. Angiotensin Ⅱ type 1 receptor autoantibody-positive female rats showed hypertensive disorder after pregnancy[J]. Journal of Capital Medical University, 2019, 40(2): 257-262.

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血管紧张素Ⅱ1型受体自身抗体阳性雌鼠孕后出现妊娠期高血压样症状

Angiotensin Ⅱ type 1 receptor autoantibody-positive female rats showed hypertensive disorder after pregnancy

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