不同亚型的帕金森病及多系统萎缩患者血浆α-突触核蛋白寡聚体浓度分析

doi:10.3969/j.issn.1006-7795.2020.02.011

首都医科大学学报 ›› 2020, Vol. 41 ›› Issue (2): 212-216.doi: 10.3969/j.issn.1006-7795.2020.02.011

不同亚型的帕金森病及多系统萎缩患者血浆α-突触核蛋白寡聚体浓度分析

宋启晗^1,2, 李旭冉^1,2, 李昕^1,2, 杨巍巍^1,2, 李伟^1,2, 于顺^1,2,3,4

1. 首都医科大学宣武医院神经生物学研究室北京市老年病医疗研究中心, 北京 100053;
2. 首都医科大学帕金森病临床诊疗与研究中心, 北京 100053;
3. 帕金森病北京市重点实验室和教育部神经变性病重点实验室, 北京 100053;
4. 国家老年疾病临床医学研究中心, 北京 100053

收稿日期:2020-01-15 出版日期:2020-04-21 发布日期:2020-04-16
通讯作者: 于顺 E-mail:yushun103@163.com
基金资助:
国家自然科学基金（81371200， 81071014， 81401042），北京市医院管理局"使命"计划专项经费资助（SML20150803），北京市科学技术委员会资助（Z161100005116011， Z171100000117013）。

Differential α-synuclein oligomerization in plasma of patients with different subtypes of Parkinson's disease and multiple system atrophy

Song Qihan^1,2, Li Xuran^1,2, Li Xin^1,2, Yang Weiwei^1,2, Li Wei^1,2, Yu Shun^1,2,3,4

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing Institute of Geriatrics, Beijing 100053, China;
2. Clinical Center for Parkinson's Disease, Capital Medical University, Beijing 100053, China;
3. Beijing Key Laboratory for Parkinson's Disease and Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 10053, China;
4. National Clinical Research Center for Geriatric Disease, Beijing 10053, China

Received:2020-01-15 Online:2020-04-21 Published:2020-04-16
Supported by:
This study was supported by National Natural Science Foundation of China （81371200， 81071014， 81401042）， Beijing Municipal Administration of Hospitals Mission Plan （SML20150803）， Beijing Municipal Science & Technology Commission （Z161100005116011， Z171100000117013）.

摘要/Abstract

摘要： 目的比较不同亚型帕金森病（Parkinson's disease，PD）和多系统萎缩（multiple system atrophy，MSA）患者血浆促进α-突触核蛋白（α-synuclein，α-Syn）寡聚体形成的能力。方法选择性别与年龄匹配的健康受试者（Control）、震颤为主型PD（tremor dominant，PD-TD）、姿势不稳与步态困难为主型PD（postural instability gait difficulty，PD-PIGD）、小脑萎缩型MSA（MSA of cerebellar type，MSA-C）和混合型MSA（MSA between cerebellar and parkinsonism type，MSA-C+P）患者各10例，抽取血浆，与重组人α-Syn振荡孵育。Western blotting法和ELISA法检测各组α-Syn寡聚体形成量。结果 PD和MSA组α-Syn寡聚体形成量显著高于对照组（P<0.05）。虽然PD组α-Syn寡聚体形成量略高于MSA组，但差异无统计学意义。亚型分析显示，α-Syn寡聚体形成量在PD-PIGD组高于PD-TD组（P<0.05），在MSA-C组高于MSA-C+P组（P<0.05）。结论 PD与MSA及其不同亚型患者血浆促进α-Syn寡聚体形成的能力不同。

关键词: 帕金森病, 多系统萎缩, α-突触核蛋白, 寡聚体, 血浆

Abstract: Objective To study α-synuclein (α-Syn) oligomerization in the plasma of patients with different subtypes of Parkinson's disease (PD) and multiple system atrophy (MSA). Methods The study enrolled 10 healthy controls, 10 tremor dominant PD patients (PD-TD), 10 postural instability/gait difficulty PD patients (PD-PIGD), 10 MSA cerebellar type (MSA-C), and 10 MSA cerebellar and parkinsonism type (MSA-C+P). The blood was collected and the plasma was isolated. Recombinant α-Syn was incubated in the plasma and the amount of α-Syn formed in the plasma was measured using ELISA method and Western blotting. Results Compared with control plasma, PD and MSA plasma significantly increased α-Syn oligomerization. Although the amount of α-Syn oligomer formed in PD plasma was slightly higher than that formed in MSA plasma, the difference was not statistically significant. In addition, α-Syn oligomerization was higher in PD-PIGD than in PD-TD plasma (P<0.05). Moreover, the ability of promoting α-Syn oligomerization was higher for MSA-C than MSA-C+P plasma (P<0.05). Conclusion The plasma of patients with different subtypes of PD and MSA differentially promotes α-Syn oligomerization.

Key words: Parkinsons disease, multiple system atrophy, α-synuclein, oligomerization, plasma

中图分类号:

R338

宋启晗, 李旭冉, 李昕, 杨巍巍, 李伟, 于顺. 不同亚型的帕金森病及多系统萎缩患者血浆α-突触核蛋白寡聚体浓度分析[J]. 首都医科大学学报, 2020, 41(2): 212-216.

Song Qihan, Li Xuran, Li Xin, Yang Weiwei, Li Wei, Yu Shun. Differential α-synuclein oligomerization in plasma of patients with different subtypes of Parkinson's disease and multiple system atrophy[J]. Journal of Capital Medical University, 2020, 41(2): 212-216.

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不同亚型的帕金森病及多系统萎缩患者血浆α-突触核蛋白寡聚体浓度分析

Differential α-synuclein oligomerization in plasma of patients with different subtypes of Parkinson's disease and multiple system atrophy

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