急性肺损伤家兔早期血管内皮生长因子的变化

首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (1): 24-27.

急性肺损伤家兔早期血管内皮生长因子的变化

聂秀红, 李晓静, 王烁, 郭德玉

首都医科大学宣武医院呼吸内科

收稿日期:2006-01-18 修回日期:1900-01-01 出版日期:2006-02-24 发布日期:2006-02-24

The Changes of Vascular Endothelial Growth Factor in the Early Period of Acute Lung Injury in Rabbit

Nie Xiuhong, Li Xiaojing, Wang Shuo, Guo Deyu

Department of Respiratory Medicine, Xuanwu Hospital, Capital University of Medical Sciences

Received:2006-01-18 Revised:1900-01-01 Online:2006-02-24 Published:2006-02-24

摘要/Abstract

摘要： 目的研究血管内皮生长因子(VEGF)在急性肺损伤(ALI)早期的变化及意义.方法将17只新西兰家兔分成2组:对照组9只,ALI组8只,建立内毒素ALI模型.测定2组动物血气、血清及肺泡灌洗液(BALF)中VEGF的质量浓度,并进行肺组织病理学观察.结果 ALI组动物注射内毒素(LPS)后出现血压、心率降低,pH值进行性下降(P均<0.05).给药后3 h,血清VEGF质量浓度明显升高,给药后6 h继续升高,与对照组相比差异有统计学意义(P<0.01).给药后6 h,BALF中VEGF质量浓度亦明显升高(P<0.01).模型动物肺组织炎性细胞浸润明显,弥散性肺泡间隔增厚,灶性出血和纤维蛋白渗出.结论内毒素所致ALI早期,VEGF迅速升高,VEGF是ALI早期较为敏感的监测指标之一.

关键词: 急性肺损伤, 内毒素, 血管内皮生长因子

Abstract: Objective To explore changes and significance of vascular endothelial growth factor(VEGF) in the early period of acute lung injury(ALI).Methods Seventeen male New Zealand rabbits were randomly divided into two groups,the control group(n=9) and the ALI group(n=8).The ALI was induced by intravenous injection of lipopolysaccaride(LPS).Blood pressure,heart rate,blood gas contents and VEGF concentration in serum and bronchoalveolar lavage fluid(BALF) were measured 3 h and 6h after LPS injection.Specimens for pathological exams were obtained at the end of each experiment.Results In ALI group,blood pressure,heart rate and pH decreased significantly,compared to control group(P<0.05).Serum and BALF VEGF concentrations were increased significantly(P<0.01).The main pathological changes observed by microscopic examination were inflammatory granulocyte infiltration,disseminated thickening of alveolar septa and local hemorrhages.There was a significant correlation between BALF VEGF concentration and the grades of pathological changes 6 h after intravenous LPS administration(r²=0.850,Y=0.070X+4.598).Conclusion LPS up-regulated serum and BALF VEGF.BALF VEGF concentration shows a high degree of correlation with pathological changes in the lung,and may serve as a useful marker for monitoring ALI.

Key words: acute lung injury, endotoxin, vascular endothelial growth factor

中图分类号:

R563

聂秀红;李晓静;王烁;郭德玉. 急性肺损伤家兔早期血管内皮生长因子的变化[J]. 首都医科大学学报, 2006, 27(1): 24-27.

Nie Xiuhong;Li Xiaojing;Wang Shuo;Guo Deyu. The Changes of Vascular Endothelial Growth Factor in the Early Period of Acute Lung Injury in Rabbit[J]. Journal of Capital Medical University, 2006, 27(1): 24-27.

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