产前应激对情绪相关脑区c-Fos神经元激活的影响

doi:10.3969/j.issn.1006-7795.2023.06.017

首都医科大学学报 ›› 2023, Vol. 44 ›› Issue (6): 1014-1021.doi: 10.3969/j.issn.1006-7795.2023.06.017

产前应激对情绪相关脑区c-Fos神经元激活的影响

李志恒¹，于鑫²，袁莹²，徐志卿^1,2*

1.首都医科大学基础医学院病理学系，北京 100069; 2.首都医科大学基础医学院神经生物学系，北京 100069

收稿日期:2023-05-16 出版日期:2023-12-21 发布日期:2023-12-20
通讯作者: 徐志卿 E-mail:zhiqingx@ccmu.edu.cn
基金资助:
国家自然科学基金项目（0100/521047）。

mpact of prenatal stress on evoked expression of c-Fos in emotion-related brain areas

Li Zhiheng¹, Yu Xin², Yuan Ying², Xu Zhiqing^1,2*

1.Department of Pathology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069,China; 2.Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2023-05-16 Online:2023-12-21 Published:2023-12-20
Supported by:
This study was supported by National Natural Science Foundation of China（0100/521047）.

摘要/Abstract

摘要： 目的探讨产前应激（prenatal stress, PS)对抑郁样行为子代大鼠情绪相关脑区c-Fos神经元激活的影响。方法利用产前束缚应激建立抑郁样行为子代大鼠模型。应用免疫荧光染色方法检测正常对照组和PS组大鼠在内侧前额叶皮质（medial prefrontal cortex，mPFC)脑区、基底外侧杏仁核（basolateral amygdala，BLA)脑区、腹侧海马（ventral hippocampus，VH)脑区和腹外侧中脑导水管周围黑质（ventrolateral periaqueductal gray，vlPAG)脑区等情绪相关脑区的c-Fos与NeuN蛋白的表达并观察上述脑区应激敏感性神经元密度和比例的改变。结果与对照组相比，PS组大鼠mPFC及BLA脑区c-Fos应激敏感性神经元密度和比例降低，VH脑区c-Fos应激敏感性神经元密度降低；vlPAG脑区c-Fos应激敏感性神经元密度不变。结论 PS导致子代大鼠mPFC、BLA及VH脑区c-Fos神经元激活密度下降，这种改变可能与抑郁样行为存在相关性。

关键词: 产前应激, c-Fos, 情绪相关脑区, 抑郁样行为

Abstract: Objective To elucidate the impact of prenatal stress (PS) on evoked expression of c-Fos in select emotion-related brain areas. Methods An experimental model of maternal exposure to chronic restraint stress in rats was employed as PS model. Subsequently, we assessed c-Fos and NeuN staining by immunofluorescence staining method in various brain regions of PS offspring rats, including the medial prefrontal cortex (mPFC), basolateral amygdala (BLA), ventral hippocampus (VH), and ventrolateral periaqueductal gray (vlPAG). Results Our findings revealed a significant decrease in the density and proportion of c-Fos positive neurons in the mPFC and BLA regions of PS-exposed offspring compared to control offspring. The density of c-Fos positive neurons in the VH region reduced in PS-exposed offspring, while no change was observed in the vlPAG region. Conclusion Our results indicated that PS induced a decreased density of stress-sensitive neurons in mPFC,BLA and VH brain regions, suggesting a potential underlying mechanism for the observed behavioral changes.

Key words: prenatal stress, c-Fos, emotion-related brain regions, depression behavior

中图分类号:

R749.4

李志恒, 于鑫, 袁莹, 徐志卿. 产前应激对情绪相关脑区c-Fos神经元激活的影响[J]. 首都医科大学学报, 2023, 44(6): 1014-1021.

Li Zhiheng, Yu Xin, Yuan Ying, Xu Zhiqing. mpact of prenatal stress on evoked expression of c-Fos in emotion-related brain areas[J]. Journal of Capital Medical University, 2023, 44(6): 1014-1021.

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产前应激对情绪相关脑区c-Fos神经元激活的影响

mpact of prenatal stress on evoked expression of c-Fos in emotion-related brain areas

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