Lsd1对小鼠调节性T细胞发育及活化的影响

doi:10.3969/j.issn.1006-7795.2023.06.016

摘要/Abstract

摘要： 目的探讨赖氨酸特异性去甲基化酶1（lysine-specific demethylase 1，Lsd1)对调节性T细胞（regulatory T cells，Treg)发育及活化的影响。方法利用条件性敲除胸腺T细胞中Lsd1的C57BL/6小鼠（Lsd1^fl/flLck-Cre)，取对照组及敲除组小鼠胸腺、脾和淋巴结，通过流式细胞术检测Treg的数量和比例，并统计效应Treg（activated effector Treg cell，eTreg)和静息Treg（naive-like central Treg cell，cTreg)比例（eTreg/cTreg)的变化。结果与对照组相比，敲除组小鼠胸腺指数减小，胸腺中CD4⁺ T细胞减少，但Treg的数量和比例增加。在外周，脾和淋巴结中Treg的比例增加，eTreg/cTreg比值增加。结论条件性敲除T细胞中Lsd1促进胸腺中Treg的发育，并可能引起脾和淋巴结中Treg的活化。

关键词: 组蛋白去甲基化酶, 赖氨酸特异性去甲基化酶1, 胸腺萎缩, 调节性T细胞

Abstract: Objective To investigate the effects of lysine-specific demethylase 1 (Lsd1) in the development and activation of regulatory T cells (Treg). Methods Thymi, spleens, and lymph nodes were isolated from control and T cell-specific Lsd1 conditional knockout (Lsd1^fl/flLck-Cre) mice. The numbers and proportions of Tregs and the ratio changes of eTreg/cTreg were measured by flow cytometry. Results Compared with the control mice, the thymus indices and numbers of thymic CD4⁺ T cells decreased in the Lsd1 knockout mice, but the numbers and proportions of thymic Treg increased. In the spleen and lymph nodes, the proportions of Treg increased, so did the eTreg/cTreg ratio. Conclusion Conditional knockout of Lsd1 in T cells promotes the development of Tregs in the thymus and the activation of Tregs in the spleen and lymph nodes.

Key words: histone demethylase, lysine-specific demethylase 1, thymic atrophy, regulatory T cells

中图分类号:

R392

夏妙然, 周航, 王达祎, 陈萍. Lsd1对小鼠调节性T细胞发育及活化的影响[J]. 首都医科大学学报, 2023, 44(6): 1006-1013.

Xia Miaoran, Zhou Hang, Wang Dayi, Chen Ping. Effects of Lsd1 on the development and activation of regulatory T cells in mice[J]. Journal of Capital Medical University, 2023, 44(6): 1006-1013.

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