基于网络药理学探究黄芩治疗慢性鼻窦炎的潜在作用机制

doi:10.3969/j.issn.1006-7795.2024.02.013

首都医科大学学报

• 慢性鼻窦炎机制和临床研究 • 上一篇下一篇

基于网络药理学探究黄芩治疗慢性鼻窦炎的潜在作用机制

杨婧兴^1,2, 赵妍^2,3, 陈陆泉⁴, 蔡超^1*#, 王向东^2,3*#

1.首都医科大学附属北京佑安医院耳鼻咽喉科，北京100069；2.首都医科大学附属北京同仁医院耳鼻咽喉头颈外科，过敏科，北京 100730； 3.北京市耳鼻咽喉科研究所,北京市中西医结合耳鼻咽喉科研究所，过敏性疾病北京实验室（北京市教育委员会），鼻病研究北京市重点实验室,耳鼻咽喉头颈科学教育部重点实验室（首都医科大学），北京100005； 4. 首都医科大学附属北京同仁医院传统医学科，北京100730

收稿日期:2024-01-23 出版日期:2024-04-25 发布日期:2024-04-25
通讯作者: 蔡超, 王向东 E-mail:fangzecai@126.com, entwxd@vip.sina.com
基金资助:
北京市自然科学基金项目（7222024），北京市科技新星交叉项目（20220484226），北京市卫健委高层次公共卫生技术人才培养计划（领军人才01-08，学科骨干03-002和02-046）。

he mechanism of Scutellaria baicalensis in treatment of chronic rhinosinusitis based on network pharmacology

Yang Jingxing^1,2, Zhao Yan^2,3, Chen Luquan⁴, Cai Chao^1*#, Wang Xiangdong^2,3*#

1.Department of Otorhinolaryngology, Beijing Youan Hospital, Capital Medical University, Beijing 100069, China； 2. Department of Otorhinolaryngology Head and Neck Surgery and Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing, 100730 China； 3. Beijing Institute of Otorhinolaryngology, Beijing Institute of Otorhinolaryngology and Integrated Traditional Chinese and Western Medicine, Beijing Laboratory of Allergic Diseases (Beijing Municipal Education Commission), Beijing Key Laboratory of Nasal Diseases, Key Laboratory of Otolaryngology Head and Neck Surgery (Capital Medical University)，Ministry of Education, Beijing 100005, China； 4. Department of Traditional Medicine, Beijing TongRen Hospital, Capital Medical University, Beijing 100730, China

Received:2024-01-23 Online:2024-04-25 Published:2024-04-25
Supported by:
This study was supported by Natural Science Foundation of Beijing (7222024), Beijing New-Star Plan of Science and Technology (20220484226), High-Level Public Health Technical Talent Training Plan (Lingjunrencai-01-08, Xuekegugan-03-002 and Xuekegugan-02-046).

摘要/Abstract

摘要： 目的运用网络药理学探究黄芩治疗慢性鼻窦炎的潜在作用机制。方法利用中药系统药理学数据库与分析平台（traditional Chinese medicine systematic pharmacology database and analysis platform, TCMSP）、中医药整合药理学研究平台（integrative pharmacology-based research platform of traditional Chinese medicine, TCMIP）和中药分子机制生物信息学分析工具（bioinformatics analysis tool for molecular mechanism of traditional Chinese medicine, BATMAN-TCM）筛选出黄芩的活性成分；利用Swiss Target Prediction数据库收集黄芩活性成分的靶点；利用GeneCards、在线人类孟德尔遗传(online Mendelian inheritance in man, OMIM)、gharmGKB和DrugBank数据库筛选“慢性鼻窦炎”相关靶点；通过STRING（search tool for the retrieval of interaction gene/proteins）数据库构建活性成分靶点与疾病靶点之间的蛋白质-蛋白质相互作用（protein-protein interaction, PPI）网络；利用Cytoscape3.10.0 软件筛选核心靶点；进行京都基因与基因组百科全书（kyoto encyclopedia of genes and genomes, KEGG）通路和基因本体（Gene Ontology, GO）富集分析以探索黄芩治疗慢性鼻窦炎的相关生物学过程及信号通路，利用Autodock tools 1.5.7将前4位关键靶点和前4个活性成分进行分子对接。结果共收集到35个黄芩活性成分，1 743个慢性鼻窦炎相关的疾病靶点，这些活性成分与慢性鼻窦炎有121个交叉靶点，通过PPI网络分析，获得28个核心靶点，4个关键靶点，GO功能富集表明黄芩治疗慢性鼻窦炎主要涉及脂多糖、蛋白酪氨酸激酶活性、膜筏、膜微区和膜区域等生物学过程，KEGG通路富集提示主要与磷脂酰肌醇3激酶/蛋白激酶B（phosphoinositide 3-kinase-serine/threonine-protein kinase, PI3K-Akt) 信号通路、高级糖基化终末产物-受体（advanced glycation end product-receptor of advanced glycation endproducts, AGE-RAGE）信号通路、Ras (reliability, availability and serviceability signaling pathway)信号通路、趋化因子信号通路等密切相关，分子对接结果显示表小檗碱与磷脂酰肌醇 4,5-二磷酸 3-激酶催化亚基 δ（phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit δ, PIK3CD）对接良好。结论本研究结果表明黄芩可能可通过多种活性成分、多个靶点及多条信号通路发挥其对慢性鼻窦炎治疗的作用。

关键词: 黄芩, 慢性鼻窦炎, 网络药理学, 作用机制

Abstract: Objective To explore the potential mechanisms of Scutellaria baicalensis in the treatment of chronic rhinosinusitis based on network pharmacology approach. Methods The active ingredients of Scutellaria baicalensis were screened on the traditional Chinese medicine systematic pharmacology database and analysis platform (TCMSP), traditional Chinese medicine integrated pharmacology research platform (TCMIP), and bioinformatics analysis tool for molecular mechanisms of traditional Chinese medicines (BATMAN-TCM). The targets of the active ingredients of Scutellaria baicalensis were collected by using the Swiss Target Prediction database； The GeneCards, online Mendelian inheritance in man (OMIM), gharmGKB and DrugBank databases were used to screen the relevant targets of chronic rhinosinusitis. The protein-protein interaction (PPI) networks between active ingredient targets and disease targets were constructed via the search tool for the retrieval of interaction gene/proteins (STRING) database. The Cytoscape 3.10.0 software was used to screen the core targets； Kyoto encyclopedia of genes and genomes (KEGG) pathway and Gene Ontology (GO) enrichment analyses were performed to explore the biological processes and signaling pathways related to the treatment of chronic rhinosinusitis with Scutellaria baicalensis. Autodock tools 1.5.7 was used to molecularly dock the top 4 key targets and the top 4 active ingredients. Results A total of 35 Scutellaria baicalensis active ingredients and 1 743 disease targets related to chronic rhinosinusitis were collected, including 121 cross-targets. A total of 28 core targets and 4 key targets were obtained by PPI network analysis. GO functional enrichment indicated that the treatment of chronic rhinosinusitis by Scutellaria baicalensis mainly involves biological processes such as lipopolysaccharide, protein tyrosine kinase activity, membrane rafts, membrane microregions and membrane regions. KEGG pathway enrichment suggests that it is mainly closely related to phosphoinositide 3-kinase-Serine/Threonine-protein kinase (PI3K-Akt) signaling pathway, advanced glycation end product-receptor of advanced glycation endproducts (AGE-RAGE) signaling pathway, reliability, availability and serviceability signaling pathway (Ras) signaling pathway, chemokine signaling pathway and so on. Epiberberine docked well with phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit δ(PIK3CD). Conclusion This study demonstrated that Scutellaria baicalensis can treat chronic rhinosinusitis through multiple active ingredients, multiple targets, and multiple signaling pathways.

Key words: Scutellaria baicalensis, chronic rhinosinusitis, network pharmacology, therapeutic mechanism

中图分类号:

R765

杨婧兴, 赵妍, 陈陆泉, 蔡超, 王向东. 基于网络药理学探究黄芩治疗慢性鼻窦炎的潜在作用机制[J]. 首都医科大学学报, doi: 10.3969/j.issn.1006-7795.2024.02.013.

Yang Jingxing, Zhao Yan, Chen Luquan, Cai Chao#, Wang Xiangdong, #. he mechanism of Scutellaria baicalensis in treatment of chronic rhinosinusitis based on network pharmacology[J]. Journal of Capital Medical University, doi: 10.3969/j.issn.1006-7795.2024.02.013.

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基于网络药理学探究黄芩治疗慢性鼻窦炎的潜在作用机制

he mechanism of Scutellaria baicalensis in treatment of chronic rhinosinusitis based on network pharmacology

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