关键词: 急性肝衰竭, 动物模型, 猪, 人工肝

Abstract: Objective To evaluate the efficacy of combined nonbioartificial liver(plasma exchange plus hemofiltration)for large animal model of acute hepatic failure induced by D-galactosamine.Methods 12 pigs were divided into two groups at random: control group(n=6) and treatment group(n=6).All of the animals were induced acute hepatic failure with adminis-tration of D-galactosamine firstly.24 h and 48 h later the animals in the treatment group were treated with plasma exchange(PE) combined with hemofiltration(HF) twice.Efficacy was assessed by comparing the survival time,improvement in manifestation,biochemical and pathological parameters.Results The treatment group's mean survival time was(112.5±14.7)h,which was much longer than that in the control group(67.9±9.4)h(P<0.01).There were significant differences for serum bilirubin,aminotransferase,ammonia,prothrombin time and histological manifestation between the two groups at the same time.Conclusion Combined nonbioartificial liver(PE plus HF) is effective for acute hepatic failure,and may be as a good bridging measure for liver transplantation and other methods.

Key words: acute hepatic failure, animal model, pig, artificial liver