首都医科大学学报 ›› 2007, Vol. 28 ›› Issue (4): 476-478.

• 基础研究 • 上一篇    下一篇

血管内皮生长因子在妊娠小鼠子宫GMG细胞中的表达

魏静波, 翁静   

  1. 首都医科大学基础医学院组织胚胎学教研室
  • 收稿日期:2006-06-14 修回日期:1900-01-01 出版日期:2007-08-24 发布日期:2007-08-24
  • 通讯作者: 翁静,Correspondingauthor,E-mail:weng.jing@163.com

Expression of Vascular Endothelial Growth Factor in the Granulated Metrial Gland Cells

Wei Jingbo, Weng Jing   

  1. Department of Histology and Embryology, School of Basic Medical Sciences, Capital Medical University
  • Received:2006-06-14 Revised:1900-01-01 Online:2007-08-24 Published:2007-08-24

摘要: 目的 探讨颗粒子宫腺(GMG)细胞中血管内皮生长因子(VEGF)的表达。方法 取妊娠13~15 d昆明种小鼠的子宫腺细胞区,经组织块培养提纯GMG细胞,应用免疫组织化学及Western blot法显示GMG细胞中VEGF。结果 组织块培养时GMG细胞生长状况好,细胞纯度高,在GMG细胞及其培养液中均有VEGF表达。结论 GMG细胞表达和分泌VEGF。

关键词: 颗粒子宫腺细胞, 血管内皮生长因子

Abstract: Objective To investigate the expression of vascular endothelial growth factor(VEGF) in the granulated metrial gland(GMG) cells.Methods Metrial glands were taken from pregnant Kunming mice at 13~15th gravid day.GMG cells were got and purified by tissue culture.Expression of VEGF in the GMG cells was shown with immunohistochemical staining and Western blot.Results GMG cells migrated from the tissue culture blots and grew well.Expression of VEGF was shown in these cells by immunohistochemical staining and Western blot.Moreover VEGF was also shown in GMG cells culture medium.Conclusion The GMG cells can express and excrete VEGF.

Key words: GMG cells, VEGF

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