Differential expressions of DNMT3B gene in glioblastoma

doi:10.3969/j.issn.1006-7795.2013.03.012

Abstract

Abstract:

Objective Global genomic hypomethylation widely exists in glioblastoma (GBM). This study aimed to clarify the molecular mechanism of this abnormality. Methods Totally 25 GBM samples were examined in this study.Total RNA was extracted from these patient samples. Full-length cDNA was amplified by RT-PCR and sequenced. Sequences obtained from each sample were compared to NCBI published standard sequences by using DNASTAR software. Results We successfully amplified DNMT3B from each patient sample. No point mutation which alters amino acids was identified. But several splicing variants were identified in these patient samples which were differentially exited in the tumor samples. Conclusion No point mutation of DNMT3B was found in GBM patient samples. Two major DNMT3B splice variants ( DNMT3b-V3 and DNMT3b-V7 ) were found differentially exist in these tumor samples. Functions of these variants are subject to further elucidation.

Key words: glioblastoma, genomic hypomethylation, DNA methyltransferase 3B

CLC Number:

R 34

BAI Yu, CHEN Yan, GUO Tianzhu, LIU Qingyang, YANG Xue, FENG Ying, YAN Zhuohong, WANG Lijia, ZHANG Quangeng. Differential expressions of DNMT3B gene in glioblastoma[J]. Journal of Capital Medical University, 2013, 34(3): 380-384.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.03.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I3/380

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