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Brief report on stroke prevention and treatment in China,2024 Stroke Prevention Project, National Health Commission, Ji Xunming Journal of Capital Medical University    2025, 46 (6): 947-960.   DOI: 10.3969/j.issn.1006-7795.2025.06.001 Abstract （4635）      PDF（pc） （5215KB）（2373）       Knowledge map    Save lthough China has a slight decline in stroke incidence in recent years, it still seems to be the leading health threat to Chinese citizens. China continues to bear a heavy stroke burden which has distinct epidemiological characteristics, while major risk factors remain prevalent and poorly controlled. Markedly, the government is putting effort into advancing the stroke prevention and treatment system, meanwhile, continuously funding cerebrovascular disease research, and driving steady improvements in diagnosis and treatment capabilities. However,  stroke prevention and control work in China still faces considerable challenges. This report systematically reviews the current state of stroke prevention and treatment in China during 2023 from five perspectives: epidemiological characteristics, risk factors, diagnostic and therapeutic advances, prevention system development and stroke center construction, aiming to provide references for clinical practice. Reference | Related Articles | Metrics

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Interpretation of the guidelines for the prevention, treatment and clinical management of iatrogenic premature ovarian insufficiency in clinical practice Zhang Mingzhen, Ruan Xiangyan, Gu Muqing, Du Juan, Cheng Jiaojiao, Alfred O. Mueck Journal of Capital Medical University    DOI: 10.3969/j.issn.1006-7795.2026.04.001 Accepted: 30 June 2026

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Micro RNA-10b regulates the early tooth development homeostasis of miniature swine by targeting and suppressing Wnt9B Sun Meng, He Xiaoli, Tong Xiangyao, Li Ang Journal of Capital Medical University    2025, 46 (6): 992-999.   DOI: 10.3969/j.issn.1006-7795.2025.06.006 Abstract （1906）      PDF（pc） （5245KB）（181）       Knowledge map    Save Objective  To investigate of the regulatory role of micro RNA (miR)-10b in early tooth development homeostasis of miniature swine and its underlying molecular mechanisms.Methods  Miniature swine bud-stage first deciduous molar tooth germs were used to perform miRNA sequencing under monoculture and mandible co-culture conditions to identify differentially expressed miRNAs. Among these, miR-10b was selected for further study, and its expression was validated by real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR). Dental mesenchymal cells were transfected with lentiviral vectors (LV) in vitro to establish a miR-10b overexpression model, and cell proliferation was evaluated using EdU and CCK-8 assays. Potential target genes of miR-10b were predicted through TargetScan and miRDB databases, and the binding sites within the 3'-untranslated region (3'-UTR) were further analyzed with RNAhybrid 2.2. To verify direct interactions, wild-type (WT) and mutant (MUT) pmirGLO dual-luciferase reporter constructs were generated, followed by dual-luciferase reporter assays and RT-qPCR to confirm the regulatory relationship.Results  Sequencing analysis revealed that miR-10b was significantly upregulated in the mandible co-culture group compared with the tooth germ monoculture group (log2FC=4.82, P<0.05), which was confirmed by RT-qPCR (2.03 ± 0.21 fold increase, P<0.001), suggesting its involvement in tooth development in miniature swine. To investigate the functional effects of miR-10b, it was overexpressed in dental mesenchymal cells, and further verified by RT-qPCR (P<0.01). EdU and CCK-8 assays showed that overexpression of miR-10b significantly inhibited the proliferation of dental mesenchymal cell. Bioinformatic analysis identified wingless-type MMTV integration site family, member 9B (Wnt9B) as a potential target gene of miR-10b and showed that specific binding sites were located within the 3'-UTR. Dual-luciferase reporter assays demonstrated direct interaction and showed that miR－10b mimics significantly decreased luciferase activity in Wnt9B-WT (P<0.01) but did not affect Wnt9B-MUT. Consequently, overexpression of miR-10b markedly downregulated Wnt9B mRNA levels in dental mesenchymal cells (P<0.01).Conclusion  miR-10b can decrease the proliferation activity of dental mesenchymal cells, regulate the homeostasis during early tooth development and maintain the balance of mandible-teeth communication by targeting and inhibiting the expression of Wnt9B in miniature swine. This study provides a new theoretical clue for revealing the molecular mechanisms of tooth development and regeneration strategies. Reference | Related Articles | Metrics

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Application of artificial intelligence in the diagnosis of urological tumors Shang Yaxin, Zhao Youquan, Xiong Tianyu, Niu Yinong, Xie Ping Journal of Capital Medical University    2026, 47 (2): 224-229.   DOI: 10.3969/j.issn.1006-7795.2026.02.002 Abstract （1704）      PDF（pc） （492KB）（524）       Knowledge map    Save Early diagnosis of urologic neoplasms is pivotal for delivering precision therapy and optimizing patient outcomes. In recent years, artificial intelligence (AI) has demonstrated remarkable potential in medical-image analysis, histopathologic image recognition, biomarker discovery, and prognostic prediction. This article systematically reviews the current status and advances of AI applications in the diagnosis of the three most common urologic cancers, including prostate cancer, bladder cancer, and renal cell cancer. By enhancing diagnostic efficiency, alleviating physicians' workload, and optimizing clinical decision-making, AI offers broad prospects. With continuous algorithmic refinement and the accumulation of multicentric data, AI is expected to play an even greater role in the precision management of urologic tumors. Reference | Related Articles | Metrics

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Effects of estrogen replacement therapy on the recovery of endocrine function after xenotransplantation of human ovarian tissue in nude mice Jiang Lingling, Ruan Xiangyan, Du Juan, Liu Anming, Cheng Jiaojiao, Gu Muqing, Li Yanglu, Zhang Mingzhen, Wang Zecheng, Li Yanqiu , Alfred O.Mueck, Journal of Capital Medical University    2026, 47 (4): 658-664.   DOI: 10.3969/j.issn.1006-7795.2026.04.004 Abstract （1684）      PDF（pc） （2257KB）（81）       Knowledge map    Save Objective  To investigate the effects of estrogen replacement therapy (ERT) on the recovery of ovarian endocrine function after human ovarian tissue xenotransplantation in nude mice. Methods  Female BALB/c nude mice aged 6-8 weeks were subjected to bilateral ovariectomy to establish a castrated model. Except for the control group, all mice underwent xenotransplantation of human ovarian cortical tissue under the renal capsule. ERT was administered via subcutaneous implantation of 17β-estradiol slow-release pellets (0.72 mg/pellet). Animals were divided into four groups according to the timing of estrogen intervention: pre-transplant estrogen pretreatment (Pre-ERT), continuous estrogen treatment (C-ERT), no estrogen treatment (No-ERT), and control group. Serum samples were collected at days 7 and 21 after transplantation, and levels of follicle-stimulating hormone (FSH), estradiol (E2), and anti-Müllerian hormone (AMH) were measured using ELISA. Results  At day 7, serum FSH levels in the control group were significantly higher than those in all transplantation groups, while the Pre-ERT group showed lower FSH levels than the No-ERT group. The C-ERT group exhibited the highest E2 levels, with all transplantation groups showing elevated E2 levels compared to the control group. AMH levels in the C-ERT group were higher than those in the No-ERT group, with all transplantation groups showed higher AMH levels than the control group (all P < 0.05). At day 21, FSH levels in the control group remained higher than those in all transplantation groups. Both Pre-ERT and C-ERT groups showed higher E2 levels than the No-ERT and control groups. AMH levels in the C-ERT group were higher than those in the No-ERT group, and all transplantation groups showing higher AMH levels relative to the control group (all P < 0.05). Conclusion  ERT improves the recovery of endocrine function after ovarian tissue xenotransplantation. Continuous estrogen treatment appears to be more effective than pre-transplant pretreatment in maintaining E2 and AMH levels, possibly by improving the local microenvironment and supporting graft function. Reference | Related Articles | Metrics

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Correlation between hemoglobin and gestational diabetes mellitus in early pregnancy Han Wenli, Zou Liying, Zheng Wei, Zhao Yue Journal of Capital Medical University    2026, 47 (1): 82-86.   DOI: 10.3969/j.issn.1006-7795.2026.01.010 Abstract （1664）      PDF（pc） （460KB）（289）       Knowledge map    Save Objective  To investigate the relationship between the hemoglobin levels in early pregnancy and risk of gestational diabetes mellitus (GDM). Methods  Clinical data of 1 044 pregnant women without preconception diabetes who gave birth in Beijing Obstetrics and Gynecology Hospital, Capital Medical University from January 1, 2024 to June 30, 2024 were selected, and their age, preconception body mass index (BMI) and the results of the first hemoglobin laboratory test in early pregnancy(at 6－10 weeks of gestations) were collected. According to the results of oral glucose tolerance test (OGTT) in the second trimester, the patients were divided into GDM group(173 cases) and non-GDM group(871 cases). Logistic regression was used to analyze the relationship between hemoglobin (HGB) and GDM in the first trimester. Results  In this study, the incidence of GDM was 16.57%, The Age, BMI and HGB in the early pregnancy of the GDM group were all higher than those of the non-GDM group, and the differences were statistically significant (P < 0.05). The HGB in the early pregnancy was significantly positively correlated with the blood glucose at 0 h (r= 0.069, P=0.025), 1h (r=0.15, P < 0.001) and 2 h (r=0.124, P<0.001) of OGTT in the mid-pregnancy. HGB concentration in the first trimester was an independent risk factor affecting the occurrence of GDM (OR=1.02,95%CI:1.004－1.046, P=0.021)，and after stratification by age and BMI,HGB levels in the early pregnancy stage remained an independent risk factor for the occurrence of GDM (all P<0.05), and the results were reliable.As the HGB level rises during the early stage of pregnancy, the risk of developing GDM increases. Conclusion  The increase of HGB level in early pregnancy is significantly correlated with the occurrence of GDM, which has predictive value for the occurrence of GDM. Reference | Related Articles | Metrics

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Research progress on the long-term health effects of intrauterine exposure to metformin during pregnancy on offspring Geng Mozhao, Zhao Yue, Zou Liying Journal of Capital Medical University    2026, 47 (1): 108-114.   DOI: 10.3969/j.issn.1006-7795.2026.01.014 Abstract （1646）      PDF（pc） （530KB）（946）       Knowledge map    Save Hyperglycemia in pregnancy is a disorder of carbohydrate metabolism that occurs during pregnancy and is associated with a variety of adverse pregnancy outcomes and an increased risk of long-term maternal and infant metabolic syndrome. Pregnant women with hyperglycemia who do not achieve the blood glucose standard despite lifestyle interventions should receive hypoglycemic drug treatment. Metformin, a classic hypoglycemic drug, has received increasing attention in its application during pregnancy. Current studies show that it has a certain effect on controlling maternal blood glucose, yet its impact on the long-term health of offspring is controversial. This review summarizes the effects of metformin exposure in utero during pregnancy on the obesity, blood pressure, lipid metabolism, endocrine system, reproductive health, and nervous system of offspring, as well as the viewpoints of guidelines from various countries on the application of metformin during pregnancy, thereby facilitating clinicians’ comprehensive understanding of the safety and rationality of metformin application during pregnancy. Reference | Related Articles | Metrics

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Construction and validation of a nomogram prediction model for the risk of adverse pregnancy outcomes in primiparous women with hypertensive disorders of pregnancy combined with gestational diabetes mellitus Lan Xueli, Zou Liying, Zhao Yue Journal of Capital Medical University    2026, 47 (1): 93-101.   DOI: 10.3969/j.issn.1006-7795.2026.01.012 Abstract （1523）      PDF（pc） （2842KB）（856）       Knowledge map    Save Objective  To screen the relevant risk factors for adverse pregnancy outcomes in pregnant women with hypertensive disorders of pregnancy（HDP） combined with gestational diabetes mellitus(GDM) and to construct risk prediction model and evaluate its predictive performance. Methods  Through the electronic medical record system, clinical data of 1 061 singleton primiparas were collected and analyzed retrospectively, who were diagnosed with HDP complicated by GDM in Beijing Obstetrics and Gynecology Hospital, Capital Medical University, from January 2022 to December 2024. A random number method was used to randomly divide the patients into a training set (742 cases) and a validation set (319 cases) according to a 7∶3 ratio. Based the pregnancy outcome the patients were divided into the adverse pregnancy outcome group (APO group) and the non-adverse pregnancy outcome group (non-APO group). The clinical data of two groups of pregnant women were compared to each other, including age, pre-pregnancy weight, pre-pregnancy body mass index (BMI), weight gain during pregnancy, pre-delivery weight, pre-delivery BMI, diabetes family history, hypertension family history, hypothyroidism, whether assisted reproductive technology was used for conception, whether pre-eclampsia (PE) or chronic hypertension with superimposed pre-eclampsia (CPE) occurred. Univariate Logistic regression analysis was used to screen variables, and a prediction model was constructed by multivariate Logistic regression analysis and a nomogram was drawn. The area under the receiver operating characteristic curve (AUC) was used to evaluate the discrimination of the model. The calibration curve and Hosmer-Lemeshow goodness—fit test were used to verify and evaluate the calibration of the model. The decision curve analysis (DCA) was used to evaluate the clinical effectiveness of the prediction model. Results  A total of 1 061 primiparas with HDP  complicated by GDM were enrolled, and the incidence of adverse pregnancy outcomes was 47.6% (505/1061). Six predictive variables were screened out by univariate Logistic regression analysis: age, pre-pregnancy BMI, hypothyroidism, diabetes family history, hypertension family history, and PE/CPE. The multivariate Logistic regression analysis indicated that they were all risk factors for the occurrence of adverse pregnancy outcomes in pregnant women with HDP complicated by GDM (P< 0.05). Draw a column line chart based on these 6 risk factors and construct a risk prediction model. The AUC of the training set patients with adverse pregnancy outcomes were 0.829 （95%CI:0.799-0.859) while that of the validation set patients with adverse pregnancy outcomes were 0.839 (95%CI:0.796-0.883), without significantly difference (P=0.477 6). The Hosmer-Lemeshow goodness-of-fit test showed a good fit (P= 0.323 8 for the training set and P= 0.702 9 for the validation set), and there was a significant agreement between the predicted value and the actual value. DCA indicated that the prediction model demonstrated clinical utility when the threshold probability exceeds 0.05. Conclusion  The risk prediction model can effectively identify high-risk groups with adverse pregnancy outcomes in primiparas with HDP complicated by GDM, which can provide reference for early intervention. Reference | Related Articles | Metrics

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Single-cell RNA sequencing identifies PTPRZ1 as a prognostic biomarker and novel therapeutic target in glioblastoma Jiang Xiaokun, Ma Jinfeng, Xie Ping, He Dong, Zhang Zhen Journal of Capital Medical University    2026, 47 (2): 251-258.   DOI: 10.3969/j.issn.1006-7795.2026.02.005 Abstract （1436）      PDF（pc） （8321KB）（169）       Knowledge map    Save Objective  To make use of single-cell RNA sequencing (scRNA-seq) technology to conduct deep analysis on glioblastoma (GBM) tumor microenvironment, identify key driver genes that have specific expression in malignant tumor cells, and assess their potential as novel therapeutic targets and prognostic biomarkers. Methods  Tumor tissues and adjacent normal tissues from three clinical GBM patients were collected for scRNA-seq. After strict data quality control, integration, normalization, and batch effect correction, cells were clustered and annotated according to cell type. Differentially expressed genes that have specific upregulation in tumor cells were screened. Loss-of-function experiments (colony formation, Transwell assays, etc.) were carried out in vitro via the GBM cell line (LN229) treated with an inhibitor (NAZ2329), so as to verify the target gene's effects on cell proliferation, viability, invasion, and migration. Protein-protein interaction networks were analyzed by use of the STRING database, and pathway enrichment analysis was implemented through Gene Set Enrichment Analysis（GSEA）. Public databases such as the Cancer Genome Atlas（TCGA） and Gene Expression Profiling Interactive Analysis（ GEPIA） were used for expression profiling and survival analysis, and immunohistochemical staining  was employed to validate target protein expression in clinical tissue samples. Results  scRNA-seq analysis identified four major cell populations: T cells, macrophages, malignant tumor cells, and oligodendrocytes. Differential expression analysis revealed that the receptor-type tyrosine phosphatase receptor-type tyrosine-protein phosphatase zeta（PTPRZ1） was one of the most significantly and specifically upregulated genes in malignant tumor cells, which was validated in an independent single-cell dataset (GSE131928). TCGA data analysis indicated that high expression of PTPRZ1 was specific to glioma. In vitro functional experiments proved that inhibition of PTPRZ1 obviously suppressed the proliferation, viability, invasion, and migration capacities of GBM cells. Bioinformatics analysis indicated that PTPRZ1 has close association with its ligand PTN, thus it may promote tumor progression via activating pathways related to nervous system development. Clinical analysis confirmed that PTPRZ1 protein has high expression in GBM tissues, and it significantly correlated with patients' poor overall survival. Conclusion  PTPRZ1 is a key molecule that has specific high expression in malignant GBM cells, it obviously promotes malignant phenotypes of GBM cells, and its high expression is associated with a poor clinical outcomes. Hence, these findings demonstrate that PTPRZ1 is a potential diagnostic biomarker. Reference | Related Articles | Metrics

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Inhibitory effects of STAT3 inhibitor WP1066 on proliferation of MYCN amplified neuroblastoma cells Jia Anna, Zhang Yao, Zhan Liping, Zhang Xuan, Zhan Shijia, Guo Jinxin, Hong Enyu, Zhang Wenxin, Guo Yongli, Chang Yan Journal of Capital Medical University    2026, 47 (2): 230-239.   DOI: 10.3969/j.issn.1006-7795.2026.02.003 Abstract （1320）      PDF（pc） （9442KB）（184）       Knowledge map    Save Objective  To investigate the inhibitory effects and molecular mechanisms of the small molecule drug WP1066 on MYCN amplified neuroblastoma (NB) cells. Methods  Among 26 pediatric drugs approved by the Food and Drug Administration (FDA) or in clinical trials, WP1066 demonstrated the most potent cytotoxic effect against MYCN amplified NB cells SK-N-BE(2). After treatment with different concentration gradients of WP1066, cell proliferation and viability were detected with CellTiter-Glo, crystal violet staining, and colony formation assays in MYCN amplified NB cells SK-N-BE(2) and IMR32, MYCN non-amplified NB cells SH-SY5Y and SK-N-AS, and human retinal pigment epithelial cells hTERT RPE-1. Apoptosis was detected with Caspase3/7 assay. The expression levels of signal transducer and activator of transcription 3(STAT3) and p-STAT3 proteins were detected with Western blotting, and the expression level of the anti-apoptotic molecule Bcl-2 mRNA was detected with real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Results  CellTiter-Glo results showed that WP1066 had a significant killing effect on MYCN-amplified NB cells SK-N-BE(2) and IMR32(P<0.05), but had no significant effect on MYCN non-amplified NB cells SH-SY5Y and SK-N-AS, and human retinal pigment epithelial cells hTERT RPE-1 at the same concentration. Crystal violet staining and colony formation experiments also showed the same results. Caspase3/7 apoptosis assay showed that WP1066 significantly promoted apoptosis in MYCN amplified NB cells SK-N-BE(2) (P<0.05), but had no significant effect on MYCN non-amplified NB cells SH-SY5Y. At the mechanism level, the small molecule drug WP1066 affected the activity of NB cells by inhibiting the p-STAT3 level in SK-N-BE(2) cells and regulating the expression level of Bcl-2(P<0.05), an anti-apoptotic molecule downstream of p-STAT3. Conclusion  WP1066 has a significant inhibitory effect on the proliferation of MYCN amplified NB cells and is expected to become a candidate therapeutic drug for MYCN amplified NB. Reference | Related Articles | Metrics

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Evaluation of the effect of characteristic electroc-acupuncture technique in treating non-infectious lower urinary tract symptoms following childbirth Zhang Quandong, Lyu Shuangxi, Zou Liying, Hua Yongqin, Yu Fengyong, Liu Hui Journal of Capital Medical University    2026, 47 (1): 102-107.   DOI: 10.3969/j.issn.1006-7795.2026.01.013 Abstract （1309）      PDF（pc） （535KB）（544）       Knowledge map    Save Objective  To evaluate the clinical efficacy of a specialized electro-acupuncture technique in treating postpartum women with non-infectious lower urinary tract symptoms (LUTS). Methods  A total of 120 primiparous women with LUTS, who were treated at the Beijing Daxing District Hospital of Integrated Chinese and Western Medicine, from January 2024 to April 2025,  were randomly assigned to the study group (n=60) and the control group (n=60). The control group received routine lifestyle guidance and pelvic floor functional training, while the study group underwent additional treatment with the specialized electro-acupuncture technique. Needles inserted into the Ciliao  acupoint and  Sanyinjiao acupoints were punctured with filiform needles, followed by electrode attachment. The treatment was administered for 30 min per session, 3 times weekly, for a total of 8 weeks. The ICIQ-FLUTS scores were collected and analyzed before and after treatment in both groups. Results  Comparison of the before and after treatment suggested that the study group showed a reduction in frequent urination (FU), urgency, dysuria, nocturia, urge urinary incontinence (UUI), stress urinary incontinence (SUI), and voiding symptoms (VS) (P<0.05), and the control group showed only a reduction in FU, nocturia, and SUI (P<0.05). Comparison within two groups after treatment suggested that the study group had a greater reduction in FU, urgency, dysuria, UUI, SUI and VS(P<0.05). The study group showed a significant reduction in severity of distress(FU, urgency, dysuria, nocturia, UUI) after the treatment.  Within  two groups after treatment, the study groups was more significant than the control group in reduction of the serious distress (P<0.05). Conclusion  The specialized electro-acupuncture technique is effective in treating postpartum women with non-infectious LUTS 42 d after delivery, which reduced the severe distress caused by FU, urgency, dysuria, nocturia, and UUI. It is worthy of clinical promotion and application. Reference | Related Articles | Metrics

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Chinese expert consensus on cytoprotection based on reperfusion therapy for acute ischemic stroke Stroke Prevention Project, National Health Commission, Stroke Prevention and Control Professional Committee of the Chinese Preventive Medicine Association Journal of Capital Medical University    2026, 47 (1): 1-9.   DOI: 10.3969/j.issn.1006-7795.2026.01.001 Abstract （1281）      PDF（pc） （648KB）（203）       Knowledge map    Save Stroke is a leading cause of mortality and disability worldwide, with approximately 70% of all cases being acute ischemic strokes. Reperfusion therapy (such as intravenous thrombolysis and emergency endovascular treatment) is the first-line treatment for acute ischemic stroke, yet the prognosis remains suboptimal for some patients. In recent years, research on neuroprotection based on reperfusion therapy has made significant progress, with some cytoprotective drugs and therapies showing promising clinical potential. This consensus summarizes recent advances in the field of cytoprotection based on reperfusion therapy and, in combination with the specific clinical and healthcare context of clinical and healthcare China, develops a practical and clinically applicable expert consensus. It aims to provide a scientific basis for cytoprotection research and clinical application in acute ischemic stroke, further optimizing treatment strategies and improving patient outcomes. Reference | Related Articles | Metrics

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The mechanism of neural modulation on the lineage differentiation of cathepsin K-positive cells in mouse alveolar bone development and homeostasis Xu Ruoshi , Peng Zhengying, Wang Yushun, Li Xuan, Pi Huaijin, Yuan Quan Journal of Capital Medical University    2025, 46 (6): 977-983.   DOI: 10.3969/j.issn.1006-7795.2025.06.004 Abstract （1273）      PDF（pc） （13037KB）（341）       Knowledge map    Save Objective  To explorethe cellular differentiation mechanisms, with  Cathepsin K (Ctsk)-positive skeletal stem cells (SSCs) lineage tracing, underlying alveolar bone tissue, periodontal ligament, and dentin development, as well as the regulatory role of peripheral nerves.Methods  Ctsk-Cre;tdTomato;Col2.3-GFP  dual-fluorescence mouse models were generated. Tissues including alveolar bone, periodontal ligament, and dental pulp were harvested from neonatal mice for Ctsk-positive lineage tracing and immunofluorescence staining. Futhermore, an inferior alveolar nerve transection model was established to analyze tdTomato+Col2.3-GFP+ double-positive cells.Results  The Ctsk-Cre;tdTomato;Col2.3-GFP fluorescent reporter mouse model was successfully generated. Lineage tracing and immunofluorescence staining in neonatal mice revealed that tdTomato+ cells in the alveolar bone expressed osterix, tdTomato+ cells in the periodontal ligament expressed Periostin, and tdTomato+ cells in the dentin expressed Col2.3-GFP.  Further analysis with the inferior alveolar nerve transection model showed reduced tdTomato+ lineage and decreased numbers of differentiating tdTomato+ Col2.3-GFP+ double-positive cells in denervated mice compared to the nerve-intact group.Conclusions  During the developmental stage of alveolar bone in neonatal mice, Ctsk positive cells participated in the development of bone tissue, periodontal ligament, and dentin, which were regulated by the inferior alveolar nerve. It shed light on injury repair and homeostasis of adulthood skeleton. Reference | Related Articles | Metrics

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Efficacy of the natural cryoprotectant L-proline versus conventional cryoprotectants in human ovarian tissue cryopreservation and transplantation Liu Anming, Ruan Xiangyan, Du Juan, Jiang Lingling, Li Yanqiu, Gu Muqing, Cheng Jiaojiao, Zhang Mingzhen, Wang Zecheng, Alfred O. Mueck, Journal of Capital Medical University    2026, 47 (4): 673-679.   DOI: 10.3969/j.issn.1006-7795.2026.04.006 Abstract （1256）      PDF（pc） （4553KB）（33）       Knowledge map    Save Objective  To evaluate the efficacy of the natural cryoprotectant L-proline (L-pro) in human ovarian tissue cryopreservation and transplantation, and to compare it with the conventional cryoprotectant dimethyl sulfoxide (DMSO).Methods  Ovarian cortical tissues obtained from ten patients were cryopreserved using a slow-freezing protocol with either DMSO-based or L-pro-based cryoprotective solutions. After thawing, the tissues were xenotransplanted into ovariectomized female nude mice, while the control group underwent ovariectomy only. Twenty-one days after transplantation, grafts were retrieved for follicle counting, and serum levels of follicle-stimulating hormone (FSH), estradiol (E2), and anti-Müllerian hormone (AMH) were measured.Results  The number of quiescent (primordial) follicles was significantly higher in the L-pro group than in the DMSO group (P=0.042), whereas no significant differences were observed in the number of growing follicles or total follicle counts between the two groups (all P> 0.05). Serum hormone levels in both experimental groups were significantly improved compared with the control group (all P<0.05), with no significant differences between the L-pro and DMSO groups.Conclusion L-pro, as a cryoprotectant, demonstrates comparable efficacy to DMSO in preserving follicular integrity and restoring endocrine function following ovarian tissue cryopreservation and xenotransplantation. These findings provide a novel perspective for optimizing cryoprotective agents in ovarian tissue preservation, although further studies are needed to elucidate the underlying mechanisms and long-term functional outcomes. Reference | Related Articles | Metrics

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Correlation analysis of thyroid peroxidase antibody and free thyroxine on the incidence of gestational diabetes mellitus Wang Chunyu, Zou Liying, Zhao Yue Journal of Capital Medical University    2026, 47 (1): 87-92.   DOI: 10.3969/j.issn.1006-7795.2026.01.011 Abstract （1242）      PDF（pc） （1044KB）（581）       Knowledge map    Save Objective  To analyze the relationship between thyroid peroxidase antibody (TPOAb), free thyroxine (FT4) in the first trimester of pregnancy and the risk of gestational diabetes mellitus (GDM). Methods  A total of 440 pregnant women who received regular prenatal check-ups and had complete data in the Department of Perinatal Medicine, Beijing Obstetrics and Gynecology Hospital, Capital Medical University from January 2020 to October 2020 were selected. They were divided into the GDM group (n=335) and the non-GDM group (n=105) according to whether GDM was diagnosed. Univariate and multivariate regression analyses were used to explore the relationship between TPOAb, FT4 in the first trimester and the occurrence of GDM. Results  The age, body mass index (BMI) and TPOAb levels of patients in the GDM group were higher than those in the non-GDM group, while the FT4 level was lower than that in the non-GDM group, with statistically significant differences (P<0.05). There was no statistically significant difference in thyroid-stimulating     hormone (TSH) level between the two groups (P>0.05). Univariate analysis showed that the occurrence of GDM was correlated with age, BMI, TPOAb and FT4 levels. Multivariate Logistic regression analysis showed that the influencing factors for the incidence of GDM were age (OR=1.111, 95%CI: 1.005-1.228), BMI (OR=1.141, 95%CI: 1.028-1.266), TPOAb level (OR=1.046, 95%CI: 1.015-1.078) and FT4 level (OR=0.749, 95%CI: 0.644-0.871). The combined area under the curve (AUC) of TPOAb and FT4 levels for predicting the prevalence of GDM was higher than that of a single indicator. Conclusion  Increased TPOAb and decreased FT4 during pregnancy are independent risk factors for GDM, and the combined detection of these two indicators has higher predictive value for the risk of GDM. It is recommended to conduct thyroid function and antibody screening for women in the first trimester of pregnancy, to provide a reference for early intervention of GDM. Reference | Related Articles | Metrics

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Study on the correlation between blood miR-181a and PGRMC1 levels and breast cancer characteristics Wang Yuejiao , Ruan Xiangyan, Gu Muqing, Wei Yun, Guan Yuwei, Alfred O.Mueck, Journal of Capital Medical University    DOI: 10.3969/j.issn.1006-7795.2026.04.008 Accepted: 30 June 2026

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Mechanisms of follicle loss after ovarian tissue cryopreservation and transplantation and the protective effect of N-acetylcysteine Li Yanglu, Ruan Xiangyan, Cheng Jiaojiao, Du Juan, Gu Muqing, Jin Fengyu, Li Yanqiu, Wang Zecheng, Zhang Mingzhen, Alfred O. Mueck, Journal of Capital Medical University    2026, 47 (4): 650-657.   DOI: 10.3969/j.issn.1006-7795.2026.04.003 Abstract （1181）      PDF（pc） （5057KB）（20）       Knowledge map    Save Objective  To investigate the mechanisms underlying follicle loss after ovarian tissue transplantation and evaluate the protective effects of N-acetylcysteine (NAC) on primordial follicles.Methods  Frozen-thawed human ovarian tissue was transplanted onto the chorioallantoic membrane (CAM) of chick embryos. Four groups were established: fresh ovarian tissue (fresh group), standardized cryopreservation and transplantation (control group), PI3K inhibitor treatment (PI3Ki group), and NAC treatment (NAC group). Follicle counts were assessed by hematoxylin-eosin staining (HE) staining, mitochondrial membrane potential (ΔΨm) was measured by JC-1 staining using flow cytometry, and the expression of phosphorylated protein kinase B (p-Akt) and phospholated forhead box o3a (p-Foxo3a) in the PI3K-Akt-Foxo3a pathway was detected by Western blotting.Results  Compared with the fresh group, all transplantation groups showed significantly reduced follicle counts (P<0.008 3) and increased mitochondrial depolarization (P<0.001). Compared with PI3Ki group, the control group exhibited activation of the PI3K-Akt-Foxo3a pathway, as indicated by elevated p-Akt and p-Foxo3a levels. This activation was suppressed in the PI3Ki group. In the NAC group, the expression of p-Akt and p-Foxo3a was significantly lower than in the control group (P<0.008 3), and the mitochondrial depolarization was the lowest among all transplanted groups(P<0.008 3).Conclusion  NAC protects primordial follicles during ovarian tissue transplantation by downregulating the PI3K-Akt-Foxo3a signaling pathway, thereby inhibiting excessive follicle activation and reducing mitochondrial damage. Reference | Related Articles | Metrics

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Mechanism of cytoskeletal regulator RACGAP1 in mediating resistance to CDK4/6 inhibitor therapy Liu Zhenzhen, Liu Weixiao, Xie Ping Journal of Capital Medical University    2026, 47 (2): 240-250.   DOI: 10.3969/j.issn.1006-7795.2026.02.004 Abstract （1175）      PDF（pc） （16576KB）（414）       Knowledge map    Save Objective  To investigate the role and molecular mechanisms of Rac GTPase-activating protein 1 (RACGAP1) in resistance to cyclin-dependent kinase 4/6 (CDK4/6) inhibitors, and to determine whether targeting RACGAP1 can synergistically enhance the efficacy of CDK4/6 inhibitors and reverse drug resistance phenotypes, thereby providing a novel therapeutic target strategy for overcoming resistance in breast cancer and other malignancies.  Methods  A Ribociclib-resistant MCF-7 sub-line was established by long-term exposure. Whole-cell pellets were subjected to RNA-seq to identify differentially expressed transcripts between parental and resistant cells. Genes consistently up-regulated across multiple CDK4/6 inhibitor models were prioritized. Public breast cancer data from The Cancer Genome Atlas (TCGA) were interrogated using the R package clusterProfiler to perform gene set enrichment analysis (GSEA) and map downstream pathways.  Results  Both RACGAP1 was markedly elevated at the mRNA and protein levels in resistant cells and in TCGA tumors, and their high expression correlated with poor response to Ribociclib. Genetic knockdown of RACGAP1 restored sensitivity to CDK4/6 inhibitors and synergistically inducing apoptosis and suppressing clonogenicity.  Conclusion  Elevated RACGAP1 drive CDK4/6 inhibitor resistance by modulating cell-cycle progression and microtubule dynamics. Targeting these genes re-sensitizes resistant breast cancer cells, offering a clinically implementable strategy to overcome CDK4/6 inhibitor resistance. Reference | Related Articles | Metrics

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Analysis of clinical and histopathological features of brucella spondylitis and diagnostic value of novel molecular pathological techniques Du Juan, Che Jialu, Yan Guangxuan, Su Dan, Che Nanying Journal of Capital Medical University    2026, 47 (3): 482-489.   DOI: 10.3969/j.issn.1006-7795.2026.03.009 Abstract （1171）      PDF（pc） （6528KB）（672）       Knowledge map    Save Objective  To analyze the clinicopathological characteristics of Brucella spondylitis (BS) and compare the pathogen detection capabilities of special staining and molecular pathology techniques, thereby providing a novel technical approach for the pathological diagnosis of BS.Methods  A retrospective analysis was conducted on the clinical data, imaging findings and histopathological features of 86 BS patients were confirmed by etiological or serological tests, who were admitted to Beijing Chest Hospital Affiliated to Capital Medical University from October 2016 to October 2025. Paraffin-embedded pathological tissue samples of these patients were subjected to Giemsa staining, weak acid-fast staining and Brucella-specific quantitative real-time polymerase chain reaction (qRT-PCR) detection.Results  Histopathological examination revealed lymphocyte and plasma cell infiltration (54.7%), eosinophil infiltration (37.2%), neutrophil infiltration (44.2%), and inflammatory granulation tissue formation (22.1%), accompanied by varying degrees of bone destruction (41.9%). In some cases, focal necrosis (12.3%), fibrous connective tissue proliferative lesions (19.8%) and sheets of histiocytes and foam cells (11.6%) were also observed, while a minority of cases showed multinucleated giant cell infiltration with granulomatous lesion formation (8.1%). The positive rate of Giemsa staining was 33.7%, and that of weak acid-fast staining was 23.3%. The results of qRT-PCR showed that 54 out of 86 cases (62.79%) were positive for Brucella-specific targets. Notably, the positive rate of IS711 (62.79%) was significantly higher than that of bcsp31 (46.51%) (P < 0.01). The combined positive detection rate of IS711 qRT-PCR and Giemsa staining was 67.44%, which was slightly higher than that of IS711 qRT-PCR alone (62.79%), without statistically significant difference (P > 0.05).Conclusion  BS presents is characterized by clinical-imaging-pathological triad. Histopathological evaluation, supplemented by special staining and molecular pathological techniques, can effectively improve the accuracy of pathological diagnosis of BS. Reference | Related Articles | Metrics

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Comparison of incisor homeostatic maintenance and injury repair in immunodeficient mice and immunocompetent mice Chang Xu, Zhang Jing, Li Juanjuan, Liu Liangliang, Shi Xuange, Shang Yutong, Wang Fu Journal of Capital Medical University    2025, 46 (6): 1011-1018.   DOI: 10.3969/j.issn.1006-7795.2025.06.008 Abstract （1150）      PDF（pc） （10705KB）（781）       Knowledge map    Save Objective  To evaluate the different responses in immunodeficient mice and immunocompetent mice during incisor homeostasis maintenance and injury repair. Methods  The mice aged 8-10 weeks were used, which include male BALB/c Nude mice (n=10), BALB/c mice (n=10), and C57BL/6J mice (n=10). A mechanical injury model was established in the incisors, and the length of the incisors was measured at a fixed time every day for three consecutive days, and their growth rate was calculated. Comparisons were made between clipped and non-clipped sides, as well as normal controls. Immunological responses were assessed, and histological analyses were conducted to evaluate mineralization in dental hard tissues. Real-time quantitative reverse transcription  polymerase chain reaction (RT-qPCR), immunohistochemistry (IHC), and immunofluorescence (IF) staining were employed to detect the expression of immune-related cytokine receptors and to assess stem cell proliferation in the cervical loop. Results  BALB/c Nude mice demonstrated significantly lower incisor growth rates under both homeostatic maintenance and injury conditions compared to BALB/c and C57BL/6J mice (P<0.05), suggesting that immune responses may contribute to incisor regeneration. Growth rates were markedly higher at night than during the day. The injury stimulated an accelerated incisor growth (P<0.05), with increased proliferation of cervical loop stem cells, as indicated by elevated numbers of SOX2+ and Ki-67+ cells (P<0.05). Histological staining revealed reduced mineralization on the clipped side compared to the unclipped and normal control side (P<0.05). Additionally, expression levels of Dspp, Ambn, genes involved in dentin and enamel formation, were significantly upregulated (P<0.05). IF staining showed that the expression of the Th2 cytokine receptor interleukin 4 receptor alpha（IL4rα）was significantly increased on the clipped side, consistent with mRNA expression levels. Conclusion  The incisor injury promotes growth and enhances stem cell proliferation and differentiation in the cervical loop. Early repair is characterized with reduced mineralization of hard tissues. This study suggests that T cell deficiency may affect the homeostatic repair and regeneration ability of incisors, and the high expression of type Ⅱ immune cytokine receptor IL4rα is closely related to the repair process. Further, it is necessary to study the specific functional mechanism to delineates the role of immune participation, thereby providing a novel theoretical framework for clinical regenerative medicine. Reference | Related Articles | Metrics