Composite piezoelectric nanoparticles based on P(VDF-TrFE) for precise imaging and targeted therapy of glioma

doi:10.3969/j.issn.1006-7795.2023.06.002

Abstract

Abstract: Objective To explore the efficacy of composite piezoelectric nanoparticles constructed based on poly（vinylidene fluoride)-trifluoroethylene ［P(VDF-TrFE)］ in the treatment and imaging of glioma. Methods P(VDF-TrFE) piezoelectric materials were reshaped in crystal form and improved in hydrophilicity, and Arg-Gly-Asp (RGD) targeted peptides were connected to 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-poly(ethylene glycol)-maleimide (DSPE-PEG-Mal) by click chemistry reaction. In addition, DSPE-PEG- tetranitroheterocyclic ligand (DSPE-PEG-DOTA) was mixed with a certain amount of gadolinium acetate solution was added to obtain DSPE-PEG-DOTA-Gd through chelation. And then the P(VDF-TrFE), DAPE-PEG-Mal RGD, and DSPE-PEG-DOTA-Gd in proportion were mixed to obtain composite piezoelectric nanoparticles P(VDF-TrFE)@DOTA-Gd@RGD. The obtained nanoparticles were characterized and assesed. Results P(VDF-TrFE)@DOTA-Gd@RGD composite piezoelectric nanoparticles have excellent stability, especially in Dulbecco's modified eagle medium (DMEM) solutions where their particle size remained around 160 nm over time. After crystal reshaping, the piezoelectric performance of P(VDF-TrFE)@DOTA-Gd@RGD is significantly improved, with a surface potential of approximately 4.2 mV. The nanoparticles could be used as a magnetic resonance imaging (MRI) agent. Under ultrasound excitation, P(VDF-TrFE)@DOTA-Gd@RGD composite piezoelectric nanoparticles generated a large amount of reactive oxygen species, inhibited the proliferation, invasion, and migration ability of glioma cells, and promoted their apoptosis. Conclusion P(VDF-TrFE)@DOTA-Gd@RGD composite piezoelectric nanoparticles could be used as an MRI agent for the localization of brain gliomas and as a sound sensitive agent for sonodynamic therapy of glioma.

Key words: glioma, piezoelectric materials, sonodynamic therapy, magnetic resonance imaging

CLC Number:

Chen Zhiguang, Zhang Wei, He Wen. Composite piezoelectric nanoparticles based on P(VDF-TrFE) for precise imaging and targeted therapy of glioma[J]. Journal of Capital Medical University, 2023, 44(6): 912-920.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2023.06.002

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2023/V44/I6/912

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