Study on the preservation with carbon oxide and heterotopic transplantation of the isolated rabbit heart

doi:10.3969/j.issn.1006-7795.2015.05.017

Abstract

Abstract: Objective Investigating the protective effect and mechanism of hypothermia (4 ℃)and desiccation with CO(CO=0.8ATA,O₂=3.2ATA,1ATA=760 mmHg, 1 mmHg=0.133 kPa)on heart preservation. Methods Seventy-two rabbits were used in this experiment; They were divided into 5 groups randomly. Group Ⅰ(eight of them were collected for examination without transplantation or preservation);Group Ⅱ(sixteen of them were divided into this group and the isolated hearts were underwent abdominal heterotopic transplantation without preservation);Group Ⅲ(sixteen of them were divided into this group and the isolated hearts were preserved with desiccation);Group Ⅳ(sixteen of them were divided into this group and the isolated hearts were preserved with desiccation and CO);Group Ⅴ(sixteen of them were divided into this group and the isolate hearts underwent 18 h preserved with HTK solution); Group Ⅲ-Ⅴ were placed in a refrigerator at 4 ℃; And then, the isolated hearts were underwent abdominal heterotopic transplantation. After the heterotopic transplantation, the re-beat rates were counted; 1 h after transplantation, the blood of receptor and the myocardium of donor were collected to measure the enzyme of function and the inflammatory factors of myocardium; Results After the experiment, most of the enzyme of function (CK-MB: creatine kinase-MB; CK: creatine kinase; LDH: lactic dehydrogenase) and the inflammatory factors of myocardium (TNF-α: tumour necrosis factor-α;IL-1: interleukin-1;IL-17: interleukin-17) in Group Ⅲ were lower than those of Group IV (P<0.05); Conclusion The heart preserved with CO was significantly better than that preserved with traditional method. And this new preservation works by reducing the inflammatory factors and alleviating the myocardia reperfusion injury.

Key words: desiccation and CO, myocardia protection, myocardia reperfusion injury, heart transplant

CLC Number:

R332
R617

Guo Yilong, Zhang Zhong, Zhou Pengyu, Zhu Ping, Huang Shuai, Zheng Shaoyi. Study on the preservation with carbon oxide and heterotopic transplantation of the isolated rabbit heart[J]. Journal of Capital Medical University, 2015, 36(5): 751-756.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.05.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I5/751

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