Doxorubicin augments adeno-associated-virus type 2 transduction in mice brain slice culture

doi:10.3969/j.issn.1006-7795.2015.06.012

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (6): 902-907.doi: 10.3969/j.issn.1006-7795.2015.06.012

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Doxorubicin augments adeno-associated-virus type 2 transduction in mice brain slice culture

Gong Xiaoli^1,2, Wang Le^2,3, Wang Wei^2,3, Fu Xia^2,3, Zhang Ting^2,3, Wang Xiaomin^2,3,4

1. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
2. Key Laboratory of Neurodegenerative Disorders of the Ministry of Education, Capital Medical University, Beijing 100069, China;
3. Department of Neurobiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
4. Beijing Institute for Brain Disorders, Beijing 100069, China

Received:2015-09-02 Online:2015-12-21 Published:2015-12-18
Supported by:
This study was supported by Major State Basic Research Development Program of China(973 Program, 2011CB504100), Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality (IDHT20140514),Projects of Beijing Municipality (TJSHG201310025006), Beijing Higher Education Young Elite Teacher Project (YETP1667).

Abstract

Abstract: Objective To investigate whether doxorubicin can be applied to increase the efficacy of AAV-2 transduction in mice brain slice culture system. Methods We co-administrated doxorubicin with AAV-2 carrying enhanced green fluorescent protein(AV2.EGFP) into the mice brain slice culture, and then observed the EGFP expression from live cells in the brain slice by microscope. Results We found that EGFP fluorescence was very low within 6 days post-infection. One micromole doxorubicin augmented AAV-2 transduction dramatically to 2.8 folds compared with control group. This effect can last at least 7 days. Moreover, similar results were obtained when doxorubicin treated at the same time with or after AV2.EGFP. Conclusion Doxorubicin augmented AAV-2 transduction in mice brain slice. Our data provided evidence to facilitate AAV-mediated gene expression in the central nervous system with the treatment of doxorubicin.

Key words: doxorubicin, brain slice, adeno-associated-virus

CLC Number:

R987.7

Gong Xiaoli, Wang Le, Wang Wei, Fu Xia, Zhang Ting, Wang Xiaomin. Doxorubicin augments adeno-associated-virus type 2 transduction in mice brain slice culture[J]. Journal of Capital Medical University, 2015, 36(6): 902-907.

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Doxorubicin augments adeno-associated-virus type 2 transduction in mice brain slice culture

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