Effect of postnatal nutritional intervention on learning and memory of intrauterine growth retardation rats

doi:10.3969/j.issn.1006-7795.2017.06.015

Abstract

Abstract: Objective In order to explore the possible developmental origins and related mechanisms of nutrition in neurodegenerative disease like Alzheimer disease (AD), we observed the changes of learning and memory ability of rats with intrauterine growth retardation (IUGR) under different nutritional intervention.Methods IUGR rats were induced by calorie restriction on pregnant rats. After birth, control (C) rats and IUGR (I) rats were fed with ad libitum food intake (A), 70% restricted food intake (R) and high fat food intake (H) respectively, and 6 groups of rats with different nutritional intervention were obtained:control with ad libitum food intake (CA), control with restricted food intake (CR), control with high fat food intake(CH), IUGR with ad libitum food intake (IA), IUGR with restricted food intake (IR) and IUGR with high fat food intake (IH). Body mass, brain mass, brain:body mass ratio, blood sugar, triglyceride and total cholesterol were determined at the period of newborn, weaning and adult. Morris water maze (MWM) was used to detect the changes of learning and memory ability in adult rats. SPSS 18.0 was applied to analyze the data. Results Compared with the control group, the blood sugar (P<0.05) and the total cholesterol (P<0.01) from newborn IUGR rats were decreased. In weaning rats, triglyceride increased in the IA and IH groups compared to all the control groups (P<0.05); total cholesterol was lower in CR group compared with CA group (P<0.05), while it was higher in CH, IA and IH groups than in CR group (P<0.05), and lower in IR group than in IA group (P<0.05). In adult rats, total cholesterol was higher in CH, IA and IH groups compared with the CA group (P<0.05). The body mass of weaning rats of CH group increased(P<0.05)and that of CR, IA and IR groups decreased(P<0.05)compared to the CA group; the brain mass in weaning rats decreased in CR, IA, IH and IR groups compared to the CA group(P<0.05); brain-to-body mass ratio in weaning rats increased in CR and IR groups(P<0.05)and decreased in CH group(P<0.05)compared to CA group. The varieties of body and brain mass in adult rats were similar to that in weaning rats. In Morris water maze test, when the platform was removed, it was detected that rats of CH and IR groups crossed the platform more frequently than the ones from CA group(P<0.05); the rats of CH group stayed in the quadrant where the platform was placed before for a longer time than the rats of CA, CR and IR group(P<0.05).Conclusion Nutritional interventions on IUGR rats resulted in the changes of blood sugar, triglyceride and total cholesterol, which thus affected the body and brain mass, which might be involved in the variety of ability of learning and memory in adult rats. It indicated that the changes in the brain of neurodegenerative disease like AD might derived from early life and be affected by the nutritional circumstance after birth.

Key words: intrauterine growth retardation, nutritional intervention, brain-body mass ratio, Morris water maze

CLC Number:

R722

Xu Shiming, Wang Hongjuan, Li Zhongqiu, Gu Tongnan, Xu Zihua, Sheng Shuli. Effect of postnatal nutritional intervention on learning and memory of intrauterine growth retardation rats[J]. Journal of Capital Medical University, 2017, 38(6): 851-856.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.06.015

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I6/851

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