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    21 November 2017, Volume 38 Issue 6
    Clinical application and challenge of liquid biopsy in the precision medicine of colorectal cancer
    Yang Yingchi, Zhang Zhongtao
    2017, 38(6):  785-789.  doi:10.3969/j.issn.1006-7795.2017.06.001
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    With the rapid development of precision medicine in cancer,the demand for dynamic monitoring of tumor molecular characteristic has been increased. In recent years, liquid biopsy is currently playing a more and more important role in diagnosis and treatment of cancer in clinical practice due to its convenience, non-invasiveness, high specificity and overcoming temporal-spatial heterogeneity. This review introduces circulating tumor cell (CTC) and circulating tumor DNA (ctDNA) as the most important technique in liquid biopsy, mainly focusing on their technological feature, especially recent research progress in colorectal cancer. Further, the challenge and possible solution are discussed in the clinic application of liquid biopsy.
    Transanal total mesorectal excision combined with intersphincteric resection for ultra-low rectal cancer
    Yao Hongwei, Chen Ning, Zhang Zhipeng, Zhang Zhongtao
    2017, 38(6):  790-794.  doi:10.3969/j.issn.1006-7795.2017.06.002
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    Objective To explore the safety and feasibility of pure transanal total mesorectal excision (pure-TaTME)combination with intersphincteric resection (ISR) for ultra-low rectal cancer.Methods The patient with ultra-low rectal cancer, who had been treated with pure-TaTME and ISR, was screened carefully by colonoscopy, endorectal ultrasound and MRI examination. A purse suture closure of the rectal lumen at the level of tumor's inferior edge was done transanally. The cut-line was made vertically to the anal canal at the level of dentate line, and further ISR procedure was performed upright into the pelvic floor.Then pure-TaTME was performed. The specimen was pulled down transanally and removed. A colo-anal anastomosis was made by use of circle stapler. Results The operation time was 350 min and the volume of blood loss was 200 mL. The length of the specimen was about 18 cm with intact mesorectum. The distal resection margin was 1.0 cm below the inferior edge of tumor.The circumferential resection margin (CRM) was negative.The pathological cancer staging was T1N0M0. Totally 32 lymph nodes had been retrieved without positive node. The postoperative recovery of patient was good without any evidence of infection and anastomotic leakage. Defecation function was acceptable and no tumor relapse was detected after a follow-up of 24 months.Conclusion The operation of pure-TaTME combined with ISR is safe, which provides a feasible option for the surgical treatment of the low or ultra-low rectal cancer. The long-term outcome need to be identified by further clinical study.
    Results of individual extralevator abdominoperineal resection for locally advanced low rectal cancer
    Han Jiagang, Wang Zhenjun, Wei Guanghui, Gao Zhigang, Yang Yong, Zhai Zhiwei, Zhao Baocheng
    2017, 38(6):  795-799.  doi:10.3969/j.issn.1006-7795.2017.06.003
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    Objective To evaluate the safety and efficacy of individualized extralevator abdominoperineal excision (ELAPE) for locally advanced low rectal cancer.Methods Fifty-six patients who underwent individualized abdominoperineal resection (APR) based on magnetic resonance imaging (MRI) from June 2011 to June 2015 were evaluated. The main outcome measures were circumferential resection margin involvement, intraoperative perforation, postoperative complications, and local recurrence. Results During the study, fifty patients (89.3%) received preoperative chemoradiotherapy.The common complications included perineal wound infection (9/56, 16.1%), sexual dysfunction (5/41, 12.2%), urinary retention (4/56, 7.1%) and chronic perineal pain (3/56, 5.4%). A positive circumferential resection margin (CRM) was demonstrated in 3 (5.4%) patients, and intraoperative perforations occurred in 2 (3.6%) patient. The local recurrence was 3.6% at a median follow-up of 35 months (range, 12-60 months).Conclusion With adequate preoperative chemoradiotherapy and MRI precise guideline, individualized ELAPE might be a relatively safe and feasible approach for locally advanced low rectal cancer with acceptable oncological outcomes.
    Diagnostic value of γ-glutamyltransferase for common bile duct stones
    Ning Xiaojing, Zhu Jiegao, Wang Dong, Zhou Xiaona, Guo Wei, Zhang Zhongtao
    2017, 38(6):  800-803.  doi:10.3969/j.issn.1006-7795.2017.06.004
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    Objective To investigate the diagnostic value of γ-glutamyltransferase (GGT) for common bile duct stones.Methods The retrospective study was conducted. The clinical data of 248 patients who were treated by laparoscopic transcysitc common bile duct exploration (LTCBDE) from January 2016 to December 2016 were collected. To evaluate the sensitivity and specificity of preoperative GGT in the diagnosis of common bile duct stones, the golden standard was the common bile duct stones were found by choledochoscopy. The receiver operating characteristic (ROC) curve was done for indicators. Results The result of preoperative detection of GGT in the diagnosis of common bile duct stones was as follows:the area under the ROC curve was 0.729, the correspondent cut-off value of diagnostic accuracy, sensitivity, specificity, positive predictive rate, negative predictive rate and the accuracy were 93.5 U/L, 78.47%, 56.73%, 21.34%-24.25%,93.72-95.95% and 69.35%, respectively; After removal of silt stones:the area under the ROC curve was 0.779, the correspondent cut-off value of diagnostic accuracy, sensitivity, specificity, positive predictive rate, negative predictive rate and the accuracy were 93.5 U/L, 74.36%, 74.04%, 24.14%-33.58%, 94.24%-96.29% and 74.2% respectively.Conclusion GGT is a sensitive indicators for the diagnosis of common bile duct stones, but it is low specific. It should be highly suspected common bile duct stones when GGT>93.5U/L, magnetic resonance cholangiopancreatography (MRCP) or endoscopic retrograde cholangiopancreatography (ERCP) should be further examined. And it can be used as one of the indications of common bile duct exploration.
    Expression and interaction of PDZK1 and PDGFR-β in adenocarcinoma of the esophagogastric junction of Siewert Ⅱ
    Pu Changsheng, Zheng Zhi, Cai Jun, Tang Huazhen, Zhang Jun, Zhang Zhongtao
    2017, 38(6):  804-809.  doi:10.3969/j.issn.1006-7795.2017.06.005
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    Objective To study the expression and interaction of PDZ kinase 1 (PDZK1) and platelet-derived growth factor receptor-β (PDGFR-β) in Siewert Ⅱ adenocarcinoma of the esophagogastric junction. To compare the expression of PDZK1 and PDGFR-β in Siewert Ⅱ adenocarcinoma of the esophagogastric junction and distal gastric cancer. To investigate whether PDZK1 is a new target of adenocarcinoma of the esophagogastric junction (AEG) targeted therapy and the basis of molecular typing.Methods Paraffin sections were prepared for immunohistochemical staining. Semi-quantitative scoring system was used to evaluate the results of immunohistochemical staining and then statistical analysis was performed. Results There was 30% (12/40) positive rate of PDZK1 in the adenocarcinoma of the esophagogastric junction. There was 95% (38/40) positive rate of PDZK1 in the normal tissues of esophagogastric junction. There was no significant difference between two tissues (P=0.268). There was 80% (32/40) positive rate of PDGFR-β in the adenocarcinoma of the esophagogastric junction. There was 28% (11/40) positive rate of PDGFR-β in the normal tissue of esophagogastric junction. There was no significant difference between two tissues (P=0.648). There was no significant correlation between PDZK1 and PDGFR-β in the normal tissue of the esophagogastric junction (r=0.026, P=0.874) and the adenocarcinoma of the esophagogastric junction (r=0.111, P=0.497). Compared with distal gastric cancer, the expression of PDZK1 in the normal tissue of the esophagogastric junction was higher than that in the distal gastric tissues (Z=-1.997,P=0.046). the expression of PDGFR-β in the adenocarcinoma tissues of the gastroesophageal junction was higher than that in the distal gastric tissues (Z=-3.811,P=0.000), there was significant difference.Conclusion There was highly expression of PDGFR-β in adenocarcinoma of the esophagogastric junction. There was highly expression of PDZK1 in normal tissues of the esophagogastric junction. There was no significant correlation between PDZK1 and PDGFR-β in the normal tissues of the esophagogastric junction and the adenocarcinoma of the esophagogastric junction. Combined with the clinical characteristics, the adenocarcinoma of esophagogastric junction may be different from the distal gastric cancer. With the determination of expression of PDZK1, it is possible to provide some reference value in AEG molecular classification and targeted therapy.
    Local anatomical and pathologic study of the structure of mesopancreas
    Xu Jingyong, Tian Xiaodong, Chen Yiran, Ma Yongsu, Zhang Weiguang, Tian Long, Wang Jianwei, Yang Yinmo
    2017, 38(6):  810-815.  doi:10.3969/j.issn.1006-7795.2017.06.006
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    Objective To investigate the anatomical and pathologic characteristics of mesopancreas.Methods A regional anatomical and pathological study was performed on 14 cadavers with large slices and paraffin sections with 12 normal samples and 2 samples from unresectable pancreatic cancer. Anatomical and pathological analysis were performed. Results Mesopancreas is a region that begins from the back of pancreatic head and neck and the left margin of uncinate process,then continues with mesentery of duodenum on the left and Gerota fascia via mesopancreatic root ontheright. The mesopancreatic root surrounded celiac trunk and superior mesentericartery containing vessels, nerves and other connective tissue. But no independent fiber sheath was found enveloping the sestructures. The tumor usually invaded inside the mesopancreas via nerves, vessels and fibers in the mesopancreatic root towards the retroperitoneum.Conclusion Mesopancreasis different from mesocolon and has no independent fascial envelop covered. Mesopancreas should be considered as a clinical concept rather than an anatomic structure to guide clinical practice.
    Influence of gasless laparoscopic common bile duct exploration on the coagulation function of patients undergone
    Ji Jiajun, Gao Xia, Fu Jianzhu, Li Guangming, Wu Jixiang
    2017, 38(6):  816-820.  doi:10.3969/j.issn.1006-7795.2017.06.007
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    Objective To investigate the influence on the coagulation function in the patients undergone gasless laparoscopic bile duct exploration.Methods Twenty-six patients undergone gasless laparoscopic common bile duct exploration were enrolled into this research. Blood samples were collected before, during and after the abdominal wall suspension, as well as the first day after operation. The following plasma molecular markers were measured:tissue-type plasminogen activator (t-PA), thrombin-antithrombin Ⅲ complexes (TAT), tissue factor activity (TF:A) and plasminogen activator inhibitor-1 (PAI-1). Results ① TF:A was significantly increased among the four groups (P<0.01). Bonferroni post hoc test indicated that TF:A of the post-suspension group was significantly increased compared to the pre-suspension group, mid-suspension group and post-operation group, respectively (P<0.01). ② TAT was not increased significantly among the four groups (P>0.05). ③ t-PA was significantly increased among the four groups (P<0.01). Bonferroni post hoc test indicated t-PA of the post-suspension group was significantly increased compared to the pre-suspension group and mid-suspension group (P<0.01). And t-PA of the post-operation group was significantly increased compared to the pre-suspension group and mid-suspension group (P<0.01). ④ PAI-1 was significantly increased among the four groups (P<0.01). Post hoc multiple comparison indicated that PAI-1 of the post-suspension group was significantly increased compared to the pre-suspension group, mid-suspension group and post-operation group, respectively (P<0.01).Conclusion Gasless laparoscopic common bile duct exploration activated the coagulation function within the controllable range of the body, and did not lead to hypercoagulation state after operation.
    Correlation between catch up growth and Alzheimer's disease
    Jin He, Zhao Zhiwei, Wang Rong
    2017, 38(6):  831-834.  doi:10.3969/j.issn.1006-7795.2017.06.011
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    The etiology and pathogenesis of Alzheimer's disease is complex and involves many factors related to the interaction of genetic and environmental. Among them, the insulin resistance, abnormal energy metabolism, oxidative stress, inflammatory response and changes in autophagy process with catch up growth are widely recognized as risk factors of Alzheimer's disease.This article focuses studies on the catch up growth phenomenon in recent years, try to explore its potential relationship with Alzheimer's disease.
    Effects of tetrahydroxystilbene glucoside on amyloid precursor protein expression in nerve cells and its mechanism related to CREB regulation
    Yin Xiaomin, Xu Ting, Dai Wufei, Qian Jiayi, Chen Chen, Zhang Lan, Li Lin
    2017, 38(6):  835-840.  doi:10.3969/j.issn.1006-7795.2017.06.012
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    Objective To explore the effects of tetrahydroxystilbene glucoside (TSG) on the expression of amyloid precursor protein (APP) and its mechanism in cultured nerve cells.Methods The expression of APP and cAMP responsive element-binding protein (CREB) was detected by Western blotting assay. The CRE cis-elements of APP promoter were analyzed by Matinspector software. The plasmids expressing serial deletion mutants of APP promoter were constructed. The luciferase activity in cells was measured by luciferase double reporter gene system. Results Activation of CREB by Forskolin down-regulated APP expression in SH-SY5Y cells. The luciferase expression plasmids of APP promoter containing CRE elements were successfully constructed. TSG treatment decreased APP level in SH-SY5Y cells, and reduced luciferase activity of APP reporter gene containing CRE1 and CRE2 cis-elements in APP promoter plasmids-transfected cells.Conclusion TSG down-regulated APP expression in nerve cells, and its mechanism may be related to affecting the binding of CREB with CRE cis-elements.
    Effects of Naoxueshu on the behavior and coagulation in the intracerebral hemorrhage in rats
    Zhang Ying, Liu Tao, Yu Rong, Zhou Ming, Yan Feng, Luo Yumin
    2017, 38(6):  841-845.  doi:10.3969/j.issn.1006-7795.2017.06.013
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    Objective To study the protective effect of Naoxueshu in the intracerebral hemorrhage in rats and its effects on the coagulation.Methods Totally 21 rats were injected with type Ⅳ collagenase were randomly divided into 3 groups, group A, group B and the control group. Group A and B were respectively given high and low concentration of Naoxueshu. We recorded the weight and assessed neurological function before sacrificing the rats at 24 hours, 48 hours, and 72 hours, and tested the coagulation parameters finally. Results The weight significantly increased at 24 h compared with that at the 72 h in group A and B (P<0.05). Compared with the control group, the weights significantly increased in group B at 72 h (P<0.05). The neurological function scores in group B were significantly decreased at 24 h, 48 h and 72 h (P<0.05). Compared with the control group, the activated partial thromboplastin time (APTT) and International Normalized Ratio (INR) significantly decreased in group B (P<0.05).Conclusion Naoxueshu could improve the weights and the neurological defects in the acute phase of intracerebral hemorrhage in rats, the mechanism maybe associated with its coagulation function.
    Brain 1H-MRS imaging characteristics in patients with overactive bladder and its correlation with clinical symptoms
    Zhou Yang, Wang Shuangkun, Zuo Long, Wang Biao, Gu Hua
    2017, 38(6):  846-850.  doi:10.3969/j.issn.1006-7795.2017.06.014
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    Objective To measure the metabolic changes of the brain using the proton magnetic resonance spectroscopy (1H-MRS) in the patients with overactive bladder (OAB).Methods We recruited 23 patients with OAB and 23 healthy controls respectively. All participants were evaluated with Self-Rating Depression Scale (SDS) and 23 patients were evaluated with Overactive Bladder Symptom Score (OABSS). Multi-voxel 1H-MRS was performed with a volume of interest located in the centrum semiovale which contains both frontal lobe and cingulate gyrus. Two main ratios of N-acetyl aspartate (NAA)/creatine (Cr) and choline (Cho)/Cr were obtained. The ratios in both frontal lobe and cingulategyrus between the two groups were compared. And the correlation between NAA/Cr, Cho/Cr and SDS, OABSS scores were analyzed. Results We found significantly lower levels of NAA/Cr ratios in both anterior frontal cortex, right medial frontal cortex, both anterior cingulate cortex and left posterior cingulate cortex in patients with OAB than in healthy controls (P<0.05). NAA/Cr in right medial frontal cortex was negatively correlated with SDS scores (r=-0.361,P<0.05).Conclusion The primary finding of the study is significant reduction in the ratios of NAA/Cr in both anterior frontal cortex, right medial frontal cortex, both anterior cingulate cortex and left posterior cingulate cortex in OAB patients, which indicates a marker of neuronal loss or dysfunction in patients with OAB.
    Effect of postnatal nutritional intervention on learning and memory of intrauterine growth retardation rats
    Xu Shiming, Wang Hongjuan, Li Zhongqiu, Gu Tongnan, Xu Zihua, Sheng Shuli
    2017, 38(6):  851-856.  doi:10.3969/j.issn.1006-7795.2017.06.015
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    Objective In order to explore the possible developmental origins and related mechanisms of nutrition in neurodegenerative disease like Alzheimer disease (AD), we observed the changes of learning and memory ability of rats with intrauterine growth retardation (IUGR) under different nutritional intervention.Methods IUGR rats were induced by calorie restriction on pregnant rats. After birth, control (C) rats and IUGR (I) rats were fed with ad libitum food intake (A), 70% restricted food intake (R) and high fat food intake (H) respectively, and 6 groups of rats with different nutritional intervention were obtained:control with ad libitum food intake (CA), control with restricted food intake (CR), control with high fat food intake(CH), IUGR with ad libitum food intake (IA), IUGR with restricted food intake (IR) and IUGR with high fat food intake (IH). Body mass, brain mass, brain:body mass ratio, blood sugar, triglyceride and total cholesterol were determined at the period of newborn, weaning and adult. Morris water maze (MWM) was used to detect the changes of learning and memory ability in adult rats. SPSS 18.0 was applied to analyze the data. Results Compared with the control group, the blood sugar (P<0.05) and the total cholesterol (P<0.01) from newborn IUGR rats were decreased. In weaning rats, triglyceride increased in the IA and IH groups compared to all the control groups (P<0.05); total cholesterol was lower in CR group compared with CA group (P<0.05), while it was higher in CH, IA and IH groups than in CR group (P<0.05), and lower in IR group than in IA group (P<0.05). In adult rats, total cholesterol was higher in CH, IA and IH groups compared with the CA group (P<0.05). The body mass of weaning rats of CH group increased(P<0.05)and that of CR, IA and IR groups decreased(P<0.05)compared to the CA group; the brain mass in weaning rats decreased in CR, IA, IH and IR groups compared to the CA group(P<0.05); brain-to-body mass ratio in weaning rats increased in CR and IR groups(P<0.05)and decreased in CH group(P<0.05)compared to CA group. The varieties of body and brain mass in adult rats were similar to that in weaning rats. In Morris water maze test, when the platform was removed, it was detected that rats of CH and IR groups crossed the platform more frequently than the ones from CA group(P<0.05); the rats of CH group stayed in the quadrant where the platform was placed before for a longer time than the rats of CA, CR and IR group(P<0.05).Conclusion Nutritional interventions on IUGR rats resulted in the changes of blood sugar, triglyceride and total cholesterol, which thus affected the body and brain mass, which might be involved in the variety of ability of learning and memory in adult rats. It indicated that the changes in the brain of neurodegenerative disease like AD might derived from early life and be affected by the nutritional circumstance after birth.
    TRPC3 participates in α-synuclein-induced mitochondrial damage
    Lu Yongquan, Chen Min, Gao Ge, Duan Chunli, Lu Lingling, Yang Hui
    2017, 38(6):  857-862.  doi:10.3969/j.issn.1006-7795.2017.06.016
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    Objective To investigate the role of transient receptor potential canonical channel 3 (TRPC3)in the mitochondrial damage induced by aberrant α-synuclein (α-syn) accumulation.Methods Expressions of TRPC3 and α-syn in mitochondria were detected by Western blotting and immunofluorescence in α-syn-overexpressing primary neurons and α-syn transgenic and knock-out mice.The detection of mitochondrial membrane potential(MMP) by JC-1 showed mitochondrial damage and the detection of cell viability used MTT and lactated dehydrogenase(LDH) release assays in α-syn-overexpression primary neurons which TRPC3 was knocked down.Results TRPC3 and α-syn co-expressed in mitochondria. Neurons overexpressing α-syn increased mitochondrial TRPC3 expression and decreased MMP and cell viability. Suppressing TRPC3 expression partially reversed the α-syn-induced reductions in MMP and cell viability.Conclusion Mitochondrial TRPC3 may participate in α-syn-induced mitochondrial damage.
    α-Synuclein promotes NMDA receptor internalization by upregulation of endocytosis protein Rab5B
    Chen Min, Yu Wenjiao, Li Xin, Yang Weiwei, Li Xuran, Yu Shun
    2017, 38(6):  863-867.  doi:10.3969/j.issn.1006-7795.2017.06.017
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    Objective To investigate the effect of α-synuclein (α-Syn) on surface N-methyl-D-aspartic acid receptors (NMDARs) in cultured rat hippocampal neurons.Methods Increased intracellular α-Syn was realized by extracellular addition of recombinant human α-Syn to the culture medium or transfection of human α-Syn gene to the neurons. Western blotting analysis was applied to observe the effects of α-Syn on levels of NMDA receptor NR1 subunit and Rab5B under conditions with or without Rab5B knockdown.Results Intracellular elevation of α-Syn decreased the surface expression of NMDA NR1 and cytoplasmic levels of Rab5B; Inhibition of Rab5B expression reversed the effects of α-Syn.Conclusion The accumulation of α-Syn in hippocampal neurons can promote the internalization of surface NMDARs through an endocytic mechanism that requires participation of Rab5B.
    α-Synuclein reduces surface expressions of NMDA receptors and NMDA-invoked Ca2+ influx and inward current by upregulation of Rab5B in cultured primary hippocampal neurons
    Yu Wenjiao, Yang Weiwei, Li Xin, Li Xuran, Chen Min, Yu Shun
    2017, 38(6):  868-873.  doi:10.3969/j.issn.1006-7795.2017.06.018
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    Objective To investigate the effects of α-synuclein(α-Syn) on surface expressions and functions of N-methyl-D-aspartate (NMDA) receptors (NMDARs) in cultured primary hippocampal neurons.Methods Increased intracellular α-Syn levels were realized by extracellular addition of recombinant human α-Syn to the culture medium of rat primary hippocampal neurons. Expressions of surface NMDARs and Rab5B were observed by fluorescence immunocytochemistry and Western blotting. Rab5B antisense oligonucleotides were applied to suppress Rab5B expression. Live cell imaging system and whole-cell patch-clamp recording were used to record NMDA-evoked Ca2+ influx and inward currents. Results Neurons treated with α-Syn displayed increased Rab5B, decreased surface NMDARs, and reduced NMDA-evoked Ca2+ influx and inward currents. The above α-Syn effects were inhibited by suppression of Rab5B expression.Conclusion α-Syn reduces the surface expression and functions of NMDARs by increasing Rab5B expression.
    Correlation between the brain and age-dependent α-synuclein phosphorylation and oligomerization in the gut of cynomolgus monkeys
    Yang Weiwei, Li Xin, Li Xuran, Yu Shun
    2017, 38(6):  874-878.  doi:10.3969/j.issn.1006-7795.2017.06.019
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    Objective To explore age-dependent alterations in oligomeric and phosphorylated α-synuclein(o-α-Syn and p-α-Syn) in the colon and brain, analyze the correlation for levels of o-α-Syn and p-α-Syn between the colon and different regions of the brain.Methods ELISA methods specific for o-α-Syn and p-α-Syn were used to measure the levels of o-α-Syn and p-α-Syn in the colon and brain of different ages of cynomolgus monkeys. Results Levels of o-α-Syn and p-α-Syn increased significantly with age in the colon and brain, statistically significant difference was observed between the old monkeys and the young and middle-aged monkeys (P< 0.01). Levels of o-α-Syn and p-α-Syn in the colon were positively correlated with those in the temporal lobe, hippocampus, striatum and thalamus of the brain.Conclusion Levels of o-α-Syn and p-α-Syn increase with age in the colon and brain and positively correlated between the colon and some vulnerable brain regions.
    Decreased α-synuclein phosphorylation and oligomerization in the plasma of patients with type 2 diabetes
    Zheng Songyang, Li Xin, Yang Weiwei, Li Xuran, Li Xuying, Yu Wenjiao, Yu Shun
    2017, 38(6):  879-883.  doi:10.3969/j.issn.1006-7795.2017.06.020
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    Objective To investigate the effect of the plasma of patients with type 2 diabetes (T2D) patient on the formation of oligomeric and phosphorylated α-synuclein (α-Syn).Methods A total of 70 hospitalized patients with T2D and 70 age-and sex-matched healthy control subjects were recruited. Blood plasmas were collected and recombinant human α-Syn was incubated with the plasma. ELISA was used to measure the levels of oligomeric and phosphorylated α-syn formed in the plasma. Results The levels of oligomeric and phosphorylated α-syn in the plasma of T2D patients were significantly lower than those in the plasma of healthy controls (P<0.05), which was positively correlated with age.Conclusion Compared with control plasma, the plasma from T2D patients decreases α-syn oligomerization and phosphorylation in an age-dependent manner.
    Bioinformatics analysis about point mutation of exon 7 in LMNA in prostate tissues and cell lines
    Zuo Lingkun, Yang Ronghui, Ma Hui, Zhou Ping, Kong Lu
    2017, 38(6):  884-890.  doi:10.3969/j.issn.1006-7795.2017.06.021
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    Objective Our research focuses on investigating the associations between point mutation of exon 7 in LMNA and its expression in prostate cancer.Methods A lamin A/C (LMNA) genomic missense variation C.1159C>CA (p.L387LI, located chr1:156106006), identified by DNA sequencing for all 12 exons of LMNA, in HPV-immobilized normal epithelium(RWPE-1)and high-invasive prostate cancer cell line(PC-3M-1E8)models was recognized. To verify these point mutations in prostate cancer patients, we downloaded three group data from PubMed GEO datasets, respectively from global RNA sequencing data, paraffin-embedded (FFPE) prostatectomy samples from 100 patients, from the transcriptome (polyA+) data of 20 prostate cancer tumors and 10 matched normal tissues and from sequencing data from 21 prostate cell lines. All reads were selected on the range from 156105950 to 156106055 on chr1 and these reads were aligned with CTACGCCTG or NTACGCCTGTCCCCCAGCCC. The length of reads analyzed is 50 nt for one time. Further, we analyzed the function of the sequence around the mutation point using GEO database. At last, we analyzed the effects of LMNA mutation by Western blotting in transfected mutation plasmid cells. Results We found that missense mutation has two forms of C/CA (p.L387LI) and C/CG (p.L387LV), however same-sense mutation has four forms of C/CT (p.L387L), C/CA (p.R388R), C/CT (p.R388R) and C/CG (p.R388R) in the range from 156106006 to 156106011 of exon 7 of chr1 in LMNA from samples or cell lines. However, the incidence of missense mutation accounted for respectively 40%, 11%, 2% and 6% in normal, Gleason score 5-6, 7 and 8-10 of patient samples. In 16 prostate cancer cell lines and 5 prostate benign cell lines, the incidence of missense mutation accounted for respectively 31% and 60% in cancer and benign cell lines. In addition, this sequence from 156106008 to 156106066 on chr1 was transcription factors (TFs) binding site; The common TFs in this region were PAX5, HEN1 (NHLH1), HTF, P53, MIF1, COMP1 and NGFIC (NGF4). By functional cluster analysis, the function of these TFs focuses on chromatin organization, positive regulation of RNA metabolic process, regulation of histone modification, regulation of chromosome organization and negative regulation of programmed cell death. Mutation led to a downregulation of in LMNA and phosphor-LMNA expression levels.Conclusion Missense mutation incidence of 156106006 on chr1 in both prostate cancer tissues and prostate cancer cell lines is lower than that of prostate normal tissue and prostate benign cell lines. It is possible to have relationship between the mutation and expression level or a heterogeneous expression pattern of LMNA.
    Influence of Houshiheisan on the expression of axon guidance factor and Rho GTPases
    Lu Yue, Liu Jianhong, Zhao Hui, Chang Jiahui, Xiang Yangyang, Zhang Qiuxia
    2017, 38(6):  891-896.  doi:10.3969/j.issn.1006-7795.2017.06.022
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    Objective To investigate the influence of Houshiheisan on the expression of axon guidance factor Netrin-1/DCC and molecular switch Rac1/Cdc42/RhoA in cerebral ischemia injury rats.Methods The permanent middle cerebral artery occlusion (pMCAO) model was made by the intraluminal suture method. Rats were randomly divided into sham group, model group, low dose of Houshiheisan group, middle dose of Houshiheisan group and Ginaton group.Western blotting and RT-PCR were employed to detect the expression of Netrin-1, DCC, Rac1, Cdc42 and RhoA in ischemic cerebral cortex of rats 7 days after operation. Results Compared with the model group,in the middle dose of Houshiheisan group and Ginaton groups, the expression of Netrin-1 protein was elevated significantly(P<0.01),middle dose of Houshiheisan group, the expression of Netrin-1 in mRNA was significantly increased(P<0.05); in the low dose of Houshiheisan, middle dose of Houshiheisan and Ginaton groups, the expression of DCC, Rac1 and Cdc42 protein and mRNA (P<0.05, P<0.01)was significantly increased; but the expression of RhoA protein and mRNA was decreased significantly (P<0.01).Conclusion The findings demonstrated that Houshiheisan could accelerate the recovery of neurological impairment after celebral ischemia by regulating the expression of axon guidance factors like Netrin-1/DCC and molecular switch Rac1/Cdc42/RhoA.
    Correlative studies between haemodynamics and sex hormone concentrations in mice with primary ovarian insufficiency
    Feng Xunxun, Dong Xiaoying, Zheng Xiurui
    2017, 38(6):  897-902.  doi:10.3969/j.issn.1006-7795.2017.06.023
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    Objective To study the correlation between haemodynamics and sex hormone concentrations in mice with premature ovarian insufficiency (POI).Methods BALB/c female mice model of POI was established by multiple sites subcutaneous injection of zona pellucida 3 of mice,and then ovaries were observed by applying ultrasound meanwhile hemodynamics changes were recorded by ultrasound color doppler. Finally, mice serum was taken to be detected.Results Under the images of ultrasound, ovaries of mice in model group experience the volume decrease, follicle decrease and low blood flow. Compared to the blank group, mice of model group have lower pulsatility index (PI), higher resistance index (RI) and an ascending trend of pulse pressure (PP). Moreover, the follicle-stimulating hormone (FSH) and anti-Müllerian hormone (AMH) in mice of model group had a significant difference (P<0.05) compared to the blank group. Then, through the correlation test between haemodynamics and sex hormone concentrations, there was a significant difference in PP and FSH (P<0.05).Conclusion The PP in haemodynamics and FSH in sex hormone concentrations have a significant difference and it can provide the base for dynamic observation in course of treatment.
    Construction and identification of gene mutation library of the N-terminal fragments of human bactericidal/permeability increasing proteins
    Li Jingqin, Kong Qingli, An Yunqing
    2017, 38(6):  903-910.  doi:10.3969/j.issn.1006-7795.2017.06.024
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    Objective To construct and identify a gene mutation library of the N-terminal fragments of human bactericidal/permeability increasing proteins (BPI23) for enhancing its activity binding lipopolysaccharides (LPS) by error-prone polymerase chain reaction (PCR) and DNA shuffling method.Methods Error-prone PCR was used to obtain random mutant fragments of BPI600 by adding different concentrations of Mg2+ and Mn2+.Random mutation rate was calculated by DNA sequencing and genetic comparison with wild-type BPI600.The product of error-prone PCR was further mutated via DNA shuffling.The reassembled product was cloned into pYD1 vector and transformed into E.coli XL10-Gold.Ten clones were randomly picked out, digested and identified by restriction enzyme HindⅢ and XhoⅠ. The positive clones were identified by DNA sequencing. Results DNA sequencing result showed that the random mutation rate was 2.3% by error-prone PCR. After DNA shuffling, 6 positive clones including BPI600 were identified in the 10 random clones selected from LB culture medium with ampicillin. Among them, 4 had 6, 9, 11 and 14 mutations, respectively; 1 had 10 mutations and 1 deletion determined by DNA sequencing. By amino acid comparison, only 2 DNA mutants were able to encode a full-length mutant BPI23 protein, with 4 and 14 amino acid mutations respectively.Conclusion A library of BPI23 mutants was constructed with a size of 2×105.The results laid the foundation for the study of its activity binding LPS.
    Comparative analysis of self-gripping mesh and mesh fixed with tacks in laparoscopic transabdominal preperitonealherniorrhaphy for giant inguinal hernia
    Wang Fan, Jin Cuihong, Zhao Fenglin, Shen Yingmo, Chen Jie
    2017, 38(6):  911-914.  doi:10.3969/j.issn.1006-7795.2017.06.025
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    Objective To analyze the self-gripping mesh and mesh fixed with tacks in laparoscopic transabdominal preperitoneal (TAPP) herniorrhaphy for giant inguinal hernia.Methods A total of 80 patients of giant inguinal hernia who were subjected to TAPP from Jan.2014 to Jan.2016 were studied. All patients were divided into two groups, including self-gripping mesh group (observation group, n=42) and mesh fixed with tacks group (control group, n=38). The operation time, hospital stay after operation, hospitalization cost, complications and visual analogue scale (VAS) after operation were observed. Results All the operations were successfully completed. There was no significant difference in the average operation time and complications, and comparing with control group, the self-gripping mesh group cost less money and caused less pain, the difference was significant (P<0.05).Conclusion Use of self-gripping mesh will decrease hospitalization cost and post hospital stay, and does not increase the risk of complications such as recurrence and it's an effectivemeasure for reducingpostoperative pain, which is worth to be popularized.
    Comparing posterior cruciate-retaining and posterior cruciate-excising total knee arthroplasty
    Wang Lin, Song Lei, Han Weifeng
    2017, 38(6):  915-918.  doi:10.3969/j.issn.1006-7795.2017.06.026
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    Objective This study was performed to compare the clinical outcomes between patients with posterior cruciate-retaining (CR) and posterior cruciate-excising (CE) total knee arthroplasty (TKA).Methods From 2007 to 2014, 56 patients (21 males and 35 females; 20 left, 36 right) with an average age of (66.56±10.33)years (from 52 to 78 years) were enrolled in this study. Patients were randomly divided into two groups, including group 1 of 34 patients subjected to CR TKA, and group 2 of 22 patients subjected to CE TKA. The evaluation parameters included operative time, blood loss, knee score and range of motion of knee.Results Fifty-six patients after an average follow-up of (25.6±7.3)months (from 12 to 36 months). Operative time was (103±21)min, (98±23)min (P>0.05). Blood loss was (361±120)mL,(355±98)mL (P>0.05). Knee score was 90.2 and 88.6 points (P>0.05). Range of motion of knee was, extension:-1.0 and-2.5 (P<0.05), flexion:111 and 112 (P>0.05). Although there was no significant difference between operative time, blood loss, knee score and the total range of motion of knee, the difference of extension range of motion of knee between the two groups was statistically significant.Conclusion Cruciate-retaining and cruciate-excising TKA function equally well postoperatively. It should be decided on the specific circumstances and the surgeons' habit.
    Through the electrical impedance tomography technology(EIT) to observe the position of the effects of mechanical ventilation in patients with ARDS lung ventilation
    Wang Yugang, Wang Hongwei, Wang Shuangshuang, Yang Weiran, Ye Gang
    2017, 38(6):  919-924.  doi:10.3969/j.issn.1006-7795.2017.06.027
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    Objective To use electrical impedance tomography technology(EIT) to observe acute respiratory distress syndrome (ARDS) patients with mechanical ventilation, in the distribution of gas in the lungs of patients with different position, and to observe the oxygen partial pressure, oxygenation index, changes of the tidal volume.Methods The prospective randomized study method will be used, the patients with ARDS in the Emergency Intensive Care Unit,Beijing Luhe Hospital,Capital Medical University in January 2015 to June 2017 were tested with lines of mechanical ventilation as the research objects, and vis application of random grouping method, the tests were divided into trial group and control group. Experimental group will be set to normal position effectively to raise 30 degrees, on the basis of daily 8 hours continuously to patients with 90 degrees of sitting position. The control group was set up 30 degrees continuously for the position. The two groups of patients were compared, position effectively maintains 72, 96, 120 hours, respectively which were collected by electrical impedance imaging region of interest (ROI) values of two groups of patients, patients which were collected through breathing machine tidal volume, through the blood gas analysis to collect oxygen partial pressure, oxygenation index parameters, such as breathing machine and use time and care in the hospital. Results Finally, a total of 60 patients were included in the analysis of 27 cases of test group and control group 33 cases. Two groups of patients with g ender, age, acute physiology and chronic health evaluation Ⅱ (APACHEⅡ) scores and lead to the causes of ARDS was no statistically significant difference. Compared with the control group, experimental group patients in position effectively maintain 96 hours and 120 hours after PaO2 and oxygenation index was significantly higher than the control group, differences were statistically significant (P<0.05), at the same time, through the observation of EIT technology experimental group patients gravity ROI3 values depend on the area for 96 hours and 120 hours, ROI4 value was significantly higher than the control group (P<0.05). Patients in the experimental group were significantly lower in ventilator use time and in the hospital than in the control group (P<0.05);the experimental group compared with the control group in position effectively maintain 96 hours and 120 hours after, tidal volume no significant change (P>0.05). Position effectively maintain 72 hours, the experimental group compared with the control group patients of oxygen partial pressure, oxygenation index, tidal volume,the difference were statistically significant (P>0.05).Conclusion For patients with ARDS line during invasive ventilation, daily continuously to 90 degrees patients keep sitting more than 8 hours, after 96 hours, can effectively improve the gravity depend on the changes of pulmonary ventilation, effectively improve the patient's oxygen partial pressure and oxygenation index, promote patients of pulmonary rehabilitation, promote patients successfully weaning tube drawing at an early date.
    Clinical analysis and experience of 4 children medulloblastoma with progressive disease during treatment
    Zhao Qian, Jin Mei, Zhang Dawei, Zhao Wen, Zhang Xue, Li Gang, Ji Yuanqi, Ma Xiaoli
    2017, 38(6):  925-929.  doi:10.3969/j.issn.1006-7795.2017.06.028
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    Objective To analyze and summarize the clinical characteristics of 4 cases of children medulloblastoma (MB) with disease progression during treatment, in order to improve the understanding of the disease, and accumulate valuable experience for future treatment.Methods The clinical manifestations, diagnosis and treatment of 4 children with MB were analyzed retrospectively from 2012 to 2014. Results Four patients (aged 3-11 years) were treated with tumor resection in our hospital. The tumor was 3.5-6.0 cm, and diagnosed as MB pathologically. Postoperative radiotherapy was performed for routine. Four patients were evaluated before chemotherapy, and all belong to grade Ⅳ, high-risk group. These 4 patients progressed at 7-9 cycles of chemotherapy with cerebrospinal dissemination. Case 1 and 2 had symptoms of intracranial hypertension and radiographic abnormalities, while case 3 and 4 only had radiographic abnormalities. There were no abnormal findings in cerebrospinal fluid cytology for all these 4 cases. In the follow-up treatment, case 1 was treated with second-line chemotherapy protocol, and was given intrathecal injection, the disease was then under control, but died of second progression after 6 months; Case 2 also shortened the chemotherapy interval, and add topotecan on the basis of second-line protocol, the disease was stable, and followed-up in outpatient; Case 3 and 4 were given prophylactic intrathecal therapy at the presence of radiographic abnormalities, follow-up with imaging examinations showed meningeal metastases were controlled. Case 3 died of intracranial primary tumor progression 3 months after chemotherapy withdrawal. Case 4 disease was stable and followed-up in outpatient.Conclusion Medulloblastoma is highly malignancy. Patients should get regular craniospinal irradiation after surgery, with vincristine every week during irradiation. Shorten the chemotherapy interval and regular monitoring of imaging and cerebrospinal fluid might prove prognosis, MRI sensitivity is better, intrathecal injection has a certain therapeutic effect on the cerebrospinal dissemination, especially in the absence of clinical manifestations, but only the cases with image changes, prophylactic intrathecal therapy can significantly improve the prognosis; On the basis of not increasing the dose of single drug, the combination of multiple drugs has certain effect on disease progression.
    Accuracy of Berlin Questionnaire in patients with pulmonary embolism
    Luo Yali, Xie Jiang
    2017, 38(6):  930-933.  doi:10.3969/j.issn.1006-7795.2017.06.029
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    Objective To investigate the accuracy of Berlin Questionnaire in patients with pulmonary embolism (PE).Methods We retrospectively compared the results of Berlin Questionnaire and sleep study for 101 and 66 subjects with and without PE.Results Totally, 74 (73.3%) PE and 46 (69.7%) non-PE patients were diagnosed as obstructive sleep apnea (OSA) by sleep study. Patients with PE demonstrated low accuracy of Berlin Questionnaire assessment, with sensitivity, specificity, positive predictive value, and negative predictive value of 70.3%, 48.1%, 78.8% and 37.1%, respectively. Among subjects objectively diagnosed of OSA, fewer PE patients complained of sleepiness than non-PE counterparts[27(36.5%) vs 26(56.5%), P=0.032]. OSA patients complicated with PE had lower Epworth Scale Score than those without PE(8.6 vs 10.3,P=0.023).Conclusion Berlin Questionnaire yields poorly to diagnose OSA in patients with PE, which may be related to low prevalence of sleepiness in this group. Objective sleep study is indicated for PE patients suspicious of sleep disordered breathing.
    Professional competencies of editors of medical academic journal
    Sun Chaoyuan, Sun Lina, Chen Ruifang, Mu Meng, Zhang Junmin
    2017, 38(6):  943-944.  doi:10.3969/j.issn.1006-7795.2017.06.032
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    Objective Medical academic journals are important because they provide means of communication and permanent record. Journal editors play pivotal roles in the process of publishing. Editors should have not only a wide range of professional knowledge, background of scientific research or clinical experience,but also strong sense of responsibility,good ability of written skills and excellent communication with people. Knowledge, skill and behavior of editors are necessary in improving academic quality of medical journal.