Expression and interaction of PDZK1 and PDGFR-β in adenocarcinoma of the esophagogastric junction of Siewert Ⅱ

doi:10.3969/j.issn.1006-7795.2017.06.005

Abstract

Abstract: Objective To study the expression and interaction of PDZ kinase 1 (PDZK1) and platelet-derived growth factor receptor-β (PDGFR-β) in Siewert Ⅱ adenocarcinoma of the esophagogastric junction. To compare the expression of PDZK1 and PDGFR-β in Siewert Ⅱ adenocarcinoma of the esophagogastric junction and distal gastric cancer. To investigate whether PDZK1 is a new target of adenocarcinoma of the esophagogastric junction (AEG) targeted therapy and the basis of molecular typing.Methods Paraffin sections were prepared for immunohistochemical staining. Semi-quantitative scoring system was used to evaluate the results of immunohistochemical staining and then statistical analysis was performed. Results There was 30% (12/40) positive rate of PDZK1 in the adenocarcinoma of the esophagogastric junction. There was 95% (38/40) positive rate of PDZK1 in the normal tissues of esophagogastric junction. There was no significant difference between two tissues (P=0.268). There was 80% (32/40) positive rate of PDGFR-β in the adenocarcinoma of the esophagogastric junction. There was 28% (11/40) positive rate of PDGFR-β in the normal tissue of esophagogastric junction. There was no significant difference between two tissues (P=0.648). There was no significant correlation between PDZK1 and PDGFR-β in the normal tissue of the esophagogastric junction (r=0.026, P=0.874) and the adenocarcinoma of the esophagogastric junction (r=0.111, P=0.497). Compared with distal gastric cancer, the expression of PDZK1 in the normal tissue of the esophagogastric junction was higher than that in the distal gastric tissues (Z=-1.997,P=0.046). the expression of PDGFR-β in the adenocarcinoma tissues of the gastroesophageal junction was higher than that in the distal gastric tissues (Z=-3.811,P=0.000), there was significant difference.Conclusion There was highly expression of PDGFR-β in adenocarcinoma of the esophagogastric junction. There was highly expression of PDZK1 in normal tissues of the esophagogastric junction. There was no significant correlation between PDZK1 and PDGFR-β in the normal tissues of the esophagogastric junction and the adenocarcinoma of the esophagogastric junction. Combined with the clinical characteristics, the adenocarcinoma of esophagogastric junction may be different from the distal gastric cancer. With the determination of expression of PDZK1, it is possible to provide some reference value in AEG molecular classification and targeted therapy.

Key words: adenocarcinoma of the esophagogastric junction, PDZ kinase 1(PDZK1), platelet-derived growth factor receptor-β(PDGFR-β), gastric cancer

CLC Number:

R656

Pu Changsheng, Zheng Zhi, Cai Jun, Tang Huazhen, Zhang Jun, Zhang Zhongtao. Expression and interaction of PDZK1 and PDGFR-β in adenocarcinoma of the esophagogastric junction of Siewert Ⅱ[J]. Journal of Capital Medical University, 2017, 38(6): 804-809.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.06.005

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I6/804

References

[1] 徐衍杰, 郑智, 刘鹏,等. 胃癌组织中CAP70和血小板源性生长因子受体β的表达及其与预后的关系[J]. 中华普通外科杂志, 2015, 30(10):813-814.
[2] Chen P H, Chen X, He X. Platelet-derived growth factors and their receptors:structural and functional perspectives[J]. Biochim Biophys Acta, 2013, 1834(10):2176-2186.
[3] Catena R, Luis-Ravelo D, Antón I, et al. PDGFR signaling blockade in marrow stroma impairs lung cancer bone metastsis[J]. Cancer Res, 2011, 71(1):164-174.
[4] Cao Y. Multifarious functions of PDGFs and PDGFRs in tumor growth and metastasis[J]. Trends Mol Med, 2013, 19(8):460-473.
[5] Appiah-Kubia K, Lana T, Wanga Y, et al. Platelet-derived growth factor receptors (PDGFRs) fusion genes involvement in hematological malignancies[J]. Crit Rev Oncol Hematol, 2017, 109:20-34.
[6] Kono S A, Heasley L E, Doebele R C, et al. Adding to the mix:fibroblast growth factor and platelet-derived growth factor receptor pathways as targets in non-small cell lung cancer[J]. Curr Cancer Drug Targets, 2012, 12(2):107-123.
[7] Frödin M, Mezheyeuski A, Corvigno S, et al. Perivascular PDGFR-β is an independent marker for prognosis in renal cell carcinoma[J]. Br J Cancer, 2017, 116(2):195-201.
[8] Manzat Saplacan R M, Balacescu L, Gherman C, et al. The role of PDGFs and PDGFRs in colorectal cancer[J]. Mediators Inflamm, 2017, 2017:4708076.
[9] Turner E C, Mulvaney E P, Reid H M, et al. Interaction of the human prostacyclin receptor with the PDZ adapter protein PDZK1:role in endothelial cell migration and angiogenesis[J]. Mol Biol Cell,2011, 22(15):2664-2679.
[10] Lee W R, Sacharidou A, Behling-Kelly E,et al. PDZK1 prevents neointima formation via suppression of breakpoint cluster region kinase in vascular smooth muscle[J]. PLoS One, 2015, 10(4):e0124494.
[11] Walther C, Caetano F A, Dunn H A, et al. PDZK1/NHERF3 differentially regulates corticotropin-releasing factor receptor 1 and serotonin 2A receptor signaling and endocytosis[J]. Cell Signal, 2015, 27(3):519-531.
[12] Zheng J, Wang L, Peng Z, et al. Low level of PDZ domain containing 1(PDZK1) predicts poor clinical outcome in patients with clear cell renal cell carcinoma[J]. EbioMedicine, 2017, 15:62-72.
[13] 徐衍杰, 张军. PDZK1通过PDGFR-β通路对胃癌发生发展的影响[J]. 国际外科学杂志, 2013, 40(7):471-474.

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[2]	Ma Zonghui, Zhang Qian, Xing Jie, Zhu Shengtao, Li Peng, Zhang Shutian, Sun Xiujing. Incidence and influence factors of endoscopic non-curative resection of early gastric cancer [J]. Journal of Capital Medical University, 2022, 43(1): 17-21.
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