Serum starvation induces gastric carcinoma chemotherapy resistance through EGFR/ERK pathway

doi:10.3969/j.issn.1006-7795.2016.01.005

Abstract

Abstract: Objective To demonstrate drug resistance induced by serum starvation in gastric cancer and explore the possible mechanism.Methods The viability of SGC7901 cells was measured by MTT assay. The inhibition rates and IC50 values were then calculated. The phosphorylation level of EGFR and ERK induced by serum starvation were measured by Western blotting. Results Serum starvation could reduce the action of chemotherapeutic drug. EGFR signal activation acted as a protective factor against starvation by regulating downstream genes. Compared with the control group, the phosphorylation levels of EGFR and ERK were significantly increased in serum starvation treatment group (P<0.05). EGFR monoclonal antibody could partially reverse multiple drug resistance as a result of serum starvation. Conclusion Serum starvation may induce drug resistance in gastric cancer cell and the mechanism may be related to the activation of EGFR/ERK signaling pathway.

Key words: gastric cancer, serum starvation, epidermal growth factor receptor(EGFR), drug resistance

CLC Number:

R656.6

Wang Junxiong, Cai Xiqiang, Nie Yongzhan, Hao Jianyu, Fan Daiming. Serum starvation induces gastric carcinoma chemotherapy resistance through EGFR/ERK pathway[J]. Journal of Capital Medical University, 2016, 37(1): 23-29.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.01.005

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I1/23

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