Table of Content

21 September 2017, Volume 38 Issue 5

Previous Issue    Next Issue

Changes of IL-4,IL-10,IL-12,IL-13, IFN-γ and TGF-β in the serum of atopic dermatitis patients at different stages of the disease

Li Yan, Xu Wei, Cheng Haiyan, Sun Xiaoli, Li Linfeng

2017, 38(5):  635-639.  doi:10.3969/j.issn.1006-7795.2017.05.001

Asbtract ( 291 )   PDF (1016KB) ( 145 )  

References | Related Articles | Metrics

Objective To investigate the changes of interleukin-4(IL-4),IL-10,IL-12,IL-13, interferon-γ (IFN-γ) and transforming growth factor-β (TGF-β) in the serum of atopic dermatitis patients at different stages of the disease, and explore their significance. Methods Seventy-nine atopic dermatitis patients at acute stage were enrolled. There were 40 cases at remission stage(remission group, RG), and 39 cases were still at acute stage after treatment(acute group, AG). The serum levels of IL-4,IL-10,IL-12,IL-13, IFN-γ and TGF-β were detected by enzyme-linked immunosorbent assay(ELISA) before and after treatment. Results There were significant differences in the serum levels of IL-4,IL-12,IL-13 and IFN-γ before and after treatment in the remission group(P<0.05). There were no significant differences in the serum levels of IL-10, TGF-β before and after treatment in the remission group(P>0.05). There were no significant differences in the serum levels of IL-4,IL-10,IL-12,IL-13, IFN-γ and TGF-β before and after treatment in the acute group(P>0.05).Conclusion Lower expression of IL-4, IL-13, and higher expression of IFN-γ, IL-12 in serum at remission stage of atopic dermatitis.

Dichotomous effect of BAFF:inducing IL-35 production by regulatory T cells

Zhang Yamin, Wang Ruiyun, Li Jun, Tao Juan, Tu Yating

2017, 38(5):  640-644.  doi:10.3969/j.issn.1006-7795.2017.05.002

Asbtract ( 168 )   PDF (4272KB) ( 122 )  

References | Related Articles | Metrics

Objective The purpose of this study was to determine whether B cell activation factor of the tumor necrosis factor (TNF) family (BAFF) has an effect on the production of interleukin-35 (IL-35) by regulatory T cells (Tregs), further identifying the dichotomous regulator of BAFF on immune responses in systemic lupus erythematosus (SLE). Methods Splenic CD4⁺ CD25⁺ T cells were sorted by magnetic isolation and stimulated with BAFF. The production of IL-35 was determined by flow cytometry and quantitative RT-PCR. Furthermore, the frequency of IL-35 producing Tregs in spleen from wild-type (WT) controls, MRL-Fas^lpr/lpr mice and anti-BAFF protein treated MRL-Fas^lpr/lpr mice was also analyzed by flow cytometry. Results We found that BAFF could promote Tregs to produce IL-35 in vitro. Flow cytometric analysis showed that the frequency of IL-35 producing Tregs was much higher in spleen of MRL-Fas^lpr/lpr mice which have increased serum level of BAFF compared with those in WT controls. Whereas anti-BAFF treatment weakened this effect. Conclusion In the pathogenesis of SLE, BAFF is not only a key pathogenic factor but also has immunosuppressive effect by promoting Tregs to produce IL-35.

Overexpression of promyelocytic leukaemia gene correlated with p53 and bcl-2 family in psoriatic peripheral lymphocytes

Peng Shiguang, Li Na, Lan Zulian, Li Li, He Yanling

2017, 38(5):  645-649.  doi:10.3969/j.issn.1006-7795.2017.05.003

Asbtract ( 178 )   PDF (2061KB) ( 107 )  

References | Related Articles | Metrics

Objective To investigate the expressions of promyelocytic leukaemia (PML) gene, p53, bcl-2 and bcl-2 associated X protein (bax) in psoriatic peripheral lymphocytes, and evaluate the relationships between PML and those candidate proteins. Methods Twenty-five psoriasis patients and 20 healthy donors were recruited. The peripheral lymphocytes were isolated. The mRNA and protein levels of PML, p53, bcl-2 and bax in peripheral lymphocytes were detected by qRT-PCR and flow cytometry, respectively. Pearson correlation matrix analysis was conducted to evaluate the relationships between PML protein and those candidate proteins.Results The mRNA and protein of PML and p53 were increased, while protein of bcl-2 decreased in peripheral lymphocytes of psoriasis patients as compared to healthy blood donors. The mRNA of bax decreased while protein of bax increased in psoriatic peripheral lymphocytes. The expression of PML protein was correlated with p53, bcl-2 and bax proteins in psoriatic peripheral lymphocytes. Conclusion PML may play a role in the mechanism of psoriasis.

Dynamic changes of CD8⁺ stem memory T cells and their effects on disease progression in chronic HIV-1 infection

Song Bingbing, Lu Xiaofan, Weng Wenjia, Su Bin, Zhang Tong, Gao Yanqing

2017, 38(5):  650-653.  doi:10.3969/j.issn.1006-7795.2017.05.004

Asbtract ( 176 )   PDF (3062KB) ( 174 )  

References | Related Articles | Metrics

Objective To study the dynamics of CD8⁺ stem memory T cells (TSCM) and the impact of CD8⁺ TSCM cells on disease progression of human immunodeficiency virus-1 (HIV-1) infection. Methods Twenty-five cases with chronic HIV-1 infection receiving antiretroviral therapy (ART) with tenofovir plus efavirenz + lamivudine and 36 healthy controls were enrolled and observed. Peripheral blood mononuclear cells were stained with using CD3-APCCy7, CD4-FITC, CD8-PerCPCy5, CD45RA-PECy7, CCR7-APC, CD27-PE, CD95-Pacific Blue, CD38-PE, HLA DR-APC monoclonal antibodies, then CD8⁺ TSCM cell percentage were determined by flow cytometry.To compare the difference of CD8⁺ TSCM cell percentage between HIV-1 chronic infected persons and healthy subjects, and to analyze the relationship between CD8⁺ TSCM cell percentage and disease progression index (CD4⁺ T cell count, HIV-1 viral load and immune activation index).Results Chronic HIV-1 infection resulted in a decrease of the CD8⁺ TSCM cell proportion in HIV-1 patients. CD8⁺ TSCM cells positively correlated with CD4⁺ T cell counts and negatively correlated with plasma viral load and CD8⁺ T cell activation. Prolonged ART successfully recovered the CD8⁺ TSCM cells, and the dynamic change of CD8⁺ TSCM cells was in parallel with CD4⁺ T cell restoration and a decrease in the level of T cell immune activation. Conclusion In summary, this report identifies CD8⁺ TSCM as a correlate of protection from disease progression. HIV-1-specific CD8 ⁺ TSCM can presumably directly contribute to the design of T cell-based vaccines.

Topical rapamycin for the treatment of facial angiofibromas in tuberous sclerosis complex

Wang Jieying, Zhu Wei, Lian Shi

2017, 38(5):  654-657.  doi:10.3969/j.issn.1006-7795.2017.05.005

Asbtract ( 210 )   PDF (970KB) ( 304 )  

References | Related Articles | Metrics

Tuberous sclerosis complex (TSC) is a kind of autosomal dominant neurocutaneous syndrome which causes hamartomatous growths in multiple organs. Facial angiofibromas occur in up to 60% of patients and can cause severe facial disfigurement. Mammalian target of rapamycin (mTOR) inhibitors such as rapamycin have been used to treat symptoms associated with the TSC. Recently, topical rapamycin has been proposed as an effective option to treat angiofibromas. In this paper, the mechanism, clinical applications, therapeutic effect and adverse reactions of rapamycin are reviewed.

Sensitization patterns for aeroallergens in self-reported allergic rhinitis within Beijing, Tianjin, and Hebei region

Lou Hongfei, Ma Siyuan, Zhao Yan, Cao Feifei, He Fei, Wang Xiangdong, Wang Chengshuo, Zhang Luo

2017, 38(5):  665-670.  doi:10.3969/j.issn.1006-7795.2017.05.008

Asbtract ( 251 )   PDF (1399KB) ( 146 )  

References | Related Articles | Metrics

Objective The aim was to assess the patterns of aeroallergen sensitizations in patients with self-reported allergic rhinitis (AR), analyze the differences of aeroallergen sensitization rates in different age groups and genders, and demonstrate the clinical relevance of positive aeroallergen with AR symptoms and allergic comorbidities. Methods A total of 6 236 patients suffering from AR symptoms in Beijing, Tianjin and Hebei region underwent standardized skin prick test (SPT) with 20 common aeroallergens during the period from January 2011 to July 2013 when seeking medical help in Beijing Tongren Hospital. Nasal symptom visual analogue score and the history of asthma and allergic conjunctivitis were recorded. Chi-square and Spearman correlation tests were used in statistical analysis. Results Among the 6 236 self-reported AR patients investigated, 5 530 (88.7%) had at least one positive skin prick reaction. The most common aeroallergen in this area were house dust mites (HDMs), with the sensitization rates (SRs) as 47.3% for Der f and 41.7% for Der p, respectively. SRs of HDMs decreased with age and were higher in males compared to females. Higher SRs of outdoor aeroallergens were observed in moderate-severe AR and persistent AR, whereas HDM SRs were not different between AR classifications. Skin index (SI) of HDMs was not relevant to AR symptoms, but SIs of mugwort and grass pollen were positively correlated with the severity of AR symptoms. More than 4 positive allergens were found in over 54% of patients with AR and comorbidities. HDMs and Blatella were not associated with AR and allergic comorbidities, whereas SRs of outdoor allergens (mugwort, dandelion and ragweed) were significantly higher in patients with AR and comorbidities compared to only AR patients. Conclusion Dust mites were the most prevalent allergens in patients with AR in Beijing, Tianjin and Hebei region. Sensitization pattern of aeroallergens varied with age and genders. Higher SRs and multiple sensitizations were associated with AR comorbidities. SRs of outdoor allergens were positively correlated with severity of AR.

Characteristic analysis of aeroallergens in Beijing patients suspicious of allergic rhinitis

Ma Siyuan, Lou Hongfei, Wang Chengshuo, Zhang Luo

2017, 38(5):  671-676.  doi:10.3969/j.issn.1006-7795.2017.05.009

Asbtract ( 150 )   PDF (1017KB) ( 123 )  

References | Related Articles | Metrics

Objective To analyze the characteristics of the main inhaled allergens in patients suspicious of allergic rhinitis (AR) with skin prick test,and provide reference for the management of AR. Methods Skin prick test was performed on patients with typical symptoms of allergic rhinitis in Beijing Tongren hospital from Jan. 1, 2011 to Jul. 31, 2013. The result of the test in different people was compared. Results 1Of the 2 598 patients, 2 292(88.2%) had positive result with clinical significance。2The most common four allergens in patients with allergic rhinitis were Dermatophagoides farina (43.1%), Dermatophagoides pteronyssinus (37.6%), Blaterlia Germanica (32.6%) and Mugwort (26.6%). Most of the patients were female, but there was no significant difference in the positive rate between the genders. 3Patients with single positive allergen (18.0%), dual positive allergens (22.6%) and triple positive allergens (19.4%) were the most common. The patients before adult had low positive rate in single positive allergen group.4 There were two peaks for the number of patients from April to June and from August to September respectively.5 In single positive allergen group, the Blaterlia Germanica has a much higher positive rate (31.6%). 6 The positive rates of dust mites varied in different age groups. Conclusion Dermatophagoides farina, Dermatophagoides pteronyssinus, Blaterlia Germanica and Mugwort are the most common four allergens in patients with allergic rhinitis, different age groups and months had different allergen distributions.

Short-term impact of severe air pollution on nasal inflammation of healthy adults

Xian Mu, Wang Kuiji, Wang Yang, Wang Chengshuo, Zhang Luo

2017, 38(5):  677-682.  doi:10.3969/j.issn.1006-7795.2017.05.010

Asbtract ( 149 )   PDF (7553KB) ( 98 )  

References | Related Articles | Metrics

Objective To investigate the impact of short-term severe air pollution on the nasal function and inflammation status of young healthy residents. Methods Twenty-three young healthy adults living in downtown Beijing were recruited. The self-reported nasal symptoms, nasal patency, upper and lower respiratory tract nitric oxide (NO) and inflammatory cytokines in the nasal secretions of each participant were recorded before and after severe air pollution which lasted for five days. Results At the second visit, different percentage of participants claimed slight symptoms of nasal congestion, irritation, dryness or postnasal drip. Accordingly, rhinometry and rhinomanometry examination showed that nasal airway resistance increased significantly, while nasal cavity volume and minimum cross-sectional area decreased significantly. Both nasal NO and exhaled NO increased after severe air pollution. In nasal secretion, Th2-type cytokines eotaxin, and interleukin-5 (IL-5), chemokine (C-C motif) ligand 7, and pro-inflammatory cytokine IL-8 increased significantly after severe air pollution, Th1-type cytokine interferon-γ (IFN-γ) as well as immunoregulatory cytokines IL-10 and transforming growth factor (TGF-β) decreased significantly. Conclusion By means of promoting the Th2-predominant immune reaction and suppressing the regulatory immune response, exposure to short-term air pollution predisposes healthy adults to nasal inflammation, especially allergic nasal inflammation.

Expression and corticosteroid inhibition of arachidonate 15-lipoxygenase in chronic rhinosinusitis with nasal polyps

Yan Bing, Wang Yang, Fan Erzhong, Li Ying, Qi Sihan, Chu Xiaohan, Wang Chengshuo, Zhang Luo

2017, 38(5):  683-687.  doi:10.3969/j.issn.1006-7795.2017.05.011

Asbtract ( 193 )   PDF (4123KB) ( 211 )  

References | Related Articles | Metrics

Objective To detect the expression of arachidonate 15-lipoxygenase (ALOX15) in eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP), and nonesinophilc chronic rhinosinusitis with nasal polyps (nonECRSwNP). Moreover, the effect of corticosteroid on ALOX15 expression was evaluated. Methods Samples were socked in RNA later and embedded in paraffin were acquired from library of Beijing Tongren Hospital. Real-time PCR and immunohistochemistry (IHC) were applied for mRNA quantification and protein distribution of ALOX15 in ECRSwNP, nonECRSwNP and healthy control group respectively.ALOX15 mRNA level of ECRSwNP samples pre-and post-corticosteroid treatment were detected. Results LOX15 mRNA level and protein expression were found increased significantly in ECRSwNP compared with nonECRSwNP and healthy control (both P=0.000). There was no significant difference between nonECRSwNP and healthy control (P=0.594, P=0.550). ALOX15 protein localized in a majority of cells including epithelia, plasma cells,neutrophils and eosinophils.One-week oral corticosteroid could decrease ALOX15 mRNA and protein expression in ECRSwNP significantly (P=0.004, P=0.024). Conclusion ALOX15 is upregulated in ECRSwNP compared with nonECRSwNP and control groups. Corticosteroid treatment could inhibit ALOX15 expression in ECRSwNP.

Association between human leukocyte antigenⅡ (HLA-Ⅱ) gene polymorphism and house dust mites-sensitive allergic rhinitis in Chinese subjects

Zhao Yanming, Wang Chengshuo, Zhao Yali, Zhang Yuan, Zhang Luo

2017, 38(5):  688-694.  doi:10.3969/j.issn.1006-7795.2017.05.012

Asbtract ( 195 )   PDF (1051KB) ( 145 )  

References | Related Articles | Metrics

Objective To elucidate the human leukocyte antigenⅡ (HLA-Ⅱ) gene alleles associated with allergic rhinitis (AR) in house dust mites sensitive (HDM-sensitive) Han Chinese subjects. Methods Seventy-one patients with AR and 92 healthy subjects were recruited to this case control study. Frequencies of HLA were estimated by using the R statistical analysis package. Significance was evaluated using χ² test and Fisher exact test. For multiple allelic frequency comparisons, Bonferroni correction was applied. Genotyping of HLA-DRB1 and HAL-DQB1 was performed by the polymerase chain reaction sequence-based genotyping method. Results A total of 16 alleles of the HLA-DRB1 locus and 13 alleles of the HLA-DQB1 locus were genotyped in the entire study cohort; of which the frequencies of alleles DQB1*06:01:01 (P=0.010, OR=2.347, 95% CI:1.392-4.225) and DRB1*08:03:02 (P=0.012, OR=3.213, 95% CI:1.732-7.821) were significantly increased in HDM-sensitive AR patients compared to healthy controls and considered to be a risk factor for AR in Chinese Han subjects. The frequency of haplotype DRB1*08:03-DQB1*06:01 in HDM-sensitive AR group was also significantly higher than that in the control group (19% vs 4.8%, P=0.007, OR=4.232;95%CI:1.822-9.237). Conclusion HLA-DRB1*08:03:02 and HLA-DQB1*06:01:01 are associated with mites-sensitive AR and may confer a risk for development of AR in Han Chinese subjects sensitized to HDM.

Non-synonymous variant rs2228145 in interleukin-6 receptor (IL-6R) gene is associated with allergic rhinitis in Chinese subjects

Zhao Yali, Zhang Yuan, Wang Chengshuo, Zhang Luo

2017, 38(5):  695-699.  doi:10.3969/j.issn.1006-7795.2017.05.013

Asbtract ( 155 )   PDF (1024KB) ( 127 )  

References | Related Articles | Metrics

Objective Allergic rhinitis (AR) is a complex chronic inflammatory disease of the nasal mucosa, caused by an interaction between genetic and environmental factors. As evidence suggests that a proportion of individuals with AR also present symptoms of asthma and some genetic variants may have increased susceptibility to both AR and asthma. The objective of this study was to identify whether asthma susceptibility variant rs2228145 in interleukin-6 receptor (IL-6R) is associated with AR in the Chinese population. Methods A cohort of 402 individuals with physician diagnosed AR and 416 healthy controls were recruited from the Han Chinese population in Beijing. DNA was extracted from the peripheral blood. rs2228145 was genotyped using the sequenom mass array technology platform. Results Analysis of frequency differences of allele between the AR patients and control subjects showed that rs2228145 in IL-6R was significantly associated with AR (P=0.0039,P_corrected=0.001 6). Genotype analysis further confirmed the difference in distribution of this variant between AR patients and controls in both the co-dominant (P_{A/C vs A/A}=0.000,OR_{A/C vs A/A}=0.546,95%CI:0.389-0.766)and dominant (P_{A/C+C/C vs A/A}=0.001,OR_{A/C+C/C vs A/A}=0.575,95%CI:0.418-0.790) models. Conclusion These results suggest that the rs2228145 in IL-6R gene is associated with decreased risk for AR.

Effects of pH values and doses on intraperitoneal injection of L-arginine induced acute pancreatitis in mice

Xiao Luyao, Zhang Jie, Zheng Shuai, Mei Shaoshuai

2017, 38(5):  700-703.  doi:10.3969/j.issn.1006-7795.2017.05.014

Asbtract ( 200 )   PDF (4787KB) ( 178 )  

References | Related Articles | Metrics

Objective To investigate the effects of different methods of L-arginine injection on acute pancreatitis model mice. Methods Mice suffering from acute pancreatitis were induced by different pH values and different doses of L-arginine, then mortality was calculated, and plasma amylase activity was detected by starch-iodine colorimetry, and pancreas and lung tissue injury severity was observed by paraffin section of HE staining at the time of 2, 4, 12, 24 h after last injection. Results Mortality in pH11 high-dose group was 100% at 2 h, and mice in other groups all survived. Compared with the control group, the plasma amylase activity and pancreas pathological score were significantly increased in all model groups (P<0.001).Compared with the other model groups, pancreas and lung tissue injury was the most severe in pH7 high-dose group. Conclusion The pH value and dosage of L-arginine solution have significant impacts on the degree of pancreatic injury. Acute pancreatitis mice model induced by 2 i.p. injections of L-arginine (400 mg/kg body weight) in 0.9% sodium chloride (pH7) at 1-hour intervals was the most severe compared with the other model groups, and with high survival rate.

Effect of propolis's solution on contaminated titanium surfaces:an in vitro study

Yang Yu, Zhou Xuan, Wang Chenbao, Wang Zuomin

2017, 38(5):  704-708.  doi:10.3969/j.issn.1006-7795.2017.05.015

Asbtract ( 171 )   PDF (3541KB) ( 184 )  

References | Related Articles | Metrics

Objective To systematically evaluate the effect of propolis's solution in killing periodontal main pathogenic bacteria,and identify the mechanisms of sterilization and decontamination of surfaces of contaminated titanium surfaces.Methods Decontamination effect of propolis on Porphyromonas gingivalis was determined by the fluid dilution method,and subsequently inoculated with the bacteria of Porphyromonas gingivalis. The bacteria in specimens were counted by colony-forming units and sterilization rate. The titanium disc were incubated in Porphyromonas gingivalis suspension.The bacterial morphology of each group were characterized by scanning electron microscopy.Results With the extension of the propolis solution concentration,reduce the number of its colonies were found. Colony count at different concentrations there were significant differences. In the comparison of 7.5 g/L,3.75 g/L,1.875 g/L after the survival of the colony count and the initial colony number have significant difference. For the different propolis solution concentrations, on average, sterilization rate had significant difference.Sterilization rate of nearly 50% on 1.875 g/L propolis's solution, 3.75 g/L of sterilization rate reached more than 60% on average, an average of 7.5 g/L sterilization rate reached more than 90%.To sum up, the colony count and average sterilization rate were associated with propolis solution concentrations. The integrity of Porphyromonas gingivalis bacterium was damaged after inoculating with propolis solution.Conclusion The findings of the study suggested that propolis's solution has good inhibitory effect on Porphyromonas gingivalis and would be a useful approach for the treatment of peri-implantitis.

Ang Ⅱ activates dendritic cell to produce immune function

Chen Chen, Meng Yan

2017, 38(5):  709-714.  doi:10.3969/j.issn.1006-7795.2017.05.016

Asbtract ( 134 )   PDF (3632KB) ( 102 )  

References | Related Articles | Metrics

Objective To investigate the immune effect of angiotensin Ⅱ(Ang Ⅱ) on immature dendritic cells (DC) compared with tumor necrosis factor-α (TNF-α)in vitro. Methods Prepared DC2.4 stimulated with different stimuli, such as 20 ng/mL TNF-α and 100 nmol Ang Ⅱ for indicated time. The effects of TNF-α and Ang Ⅱ on the proliferation, maturation, phagocytosis, cytokine secretion, migration, and communication with T cells of DCs are test by MTT, flow cytometry, ELISA, transwell assay and mixed lymphocyte culture respectively. Results First, the data showed that Ang Ⅱ stimulation significantly inhibited proliferation of DC, induced phenotypic maturation of DC, markedly increased the migration of DC, inhibited macrophage phagocytosis, promoted the secretion of interleukin-6 (IL-6) and interferon-γ(IFN-γ) and initiated the proliferation and activation of T-cell. Conclusion Compared with TNF-α, Ang Ⅱ positively modulated the function of DC to the same extent.

Analysis of subjects development of universities based on ESI and InCites:a case study of Capital Medical University

Liu Yuting, Ma Lu, Huang Fang, Gong Jiajian

2017, 38(5):  715-726.  doi:10.3969/j.issn.1006-7795.2017.05.017

Asbtract ( 314 )   PDF (5443KB) ( 110 )  

References | Related Articles | Metrics

Objective To investigate situation of the Capital Medical University's (CCMU) Essential Science Indicators (ESI) research areas. Methods Using ESI, InCites, we will analyze the numbers,citations, percentage of citations, Category Normalized Citation Impact (CNCI), H index, and co-operation status of CCMU Web of Science articles. Results CCMU had five ESI research areas(clinical medicine, neuroscience & behavior, immunology, pharmacology & toxicology, biology & biochemistry), the quality of published journals should be improved, supported mainly by the National Natural Science Foundation and the Natural Science Foundation of Beijing, institutional cooperation mainly in the domestic and international cooperation is mainly concentrated in the United States. Conclusion To promote the first-class disciplines construction,Capital Medical University needs to strengthen discipline layout, to enhance academic influence, to pay more attention to the quality of the articles, to encourage teachers to get high-level papers published, to participate in international cooperation and to expand international academic influence, and take into account academic cooperation and academic impact assessment when evaluating introduction of academic talents.

Risk factors on anastomotic leakage after low anterior resection of rectal cancer

Xu Yi, Zhao Xiaomu, Sun Wuqing, Ma Jian, Wang Yueyue, Wang Jin

2017, 38(5):  727-732.  doi:10.3969/j.issn.1006-7795.2017.05.018

Asbtract ( 138 )   PDF (1005KB) ( 125 )  

References | Related Articles | Metrics

Objective To investigate the risk factors on anastomotic leakage after low anterior resection of rectal cancer so as to provide a basis for the prevention and treatment of anastomotic leakage, thus reducing the incidence of leakage. Methods The data of 261 rectal cancer patients undergoing low anterior resection from January 1st 2013 to March 31st 2017 were recruited. All patients experienced a total mesorectal excision (TME) operation. χ² test was performed for univariate analysis, and Logistic regression test was carried out for multivariate correlation analysis. Results The incidence of anastomotic leakage was 6.89% (18/261). Multivariate analysis showed that the occurrence of anastomotic leakage was closely correlated to neoadjuvant chemoradiation (OR=3.498,P=0.024) and combined with other organ surgery (OR=10.834, P=0.003). Conclusion Neoadjuvant chemoradiation and combined with other organ surgery were considered as independent risk factors for anastomotic leakage after anterior resection of rectal cancer. According to the relevant risk factors, the effective measures should be taken to reduce the incidence of anastomotic leakage.

Detail of the en-bloc technique and prognosis of spleen-preserving laparoscopic distal pancreatectomy for pancreatic cancer

Sun Zhipeng, Zhu Yubing, Aminbuhe, Fan Qing, Li Tianxiong, Zhang Nengwei

2017, 38(5):  733-739.  doi:10.3969/j.issn.1006-7795.2017.05.019

Asbtract ( 240 )   PDF (9480KB) ( 164 )  

References | Related Articles | Metrics

Objective The aim of our study was to illustrate the detail of the spleen-preserving en-bloc technique as while as the short-term, long-term outcomes. Methods Describe the detail of the en-bloc technique with pictures. Evaluate the prognosis of successive 23 cases who underwent the laparoscopic distal pancreatectomy (LDP) surgery. There were 17 cases that underwent spleen-preserving LDP while 6 cases underwent spleen-resecting LDP. Results The average surgery time was (203 ±54)minutes, the average blood loss volume was (208 ±106)mL. One case was transferred to open surgery because of severe adhesion. The complication rate was 47% (n=8) in short-term after surgery. Pancreatic fistula rate was 41% (n=7). No lethal case occurred. The average diameter of the tumor was (32 ±12)mm. Average number of the lymph nodes obtained was (19.8 ±9.3). All the cutting edges were negative. Survival rate of the patient after 1, 3, 5 years were 64.7%, 52.9% and 41.2%. These records showed no statistical significance compared with spleen-resecting LDP and open distal pancreatectomy (ODP) surgeries. Conclusion The en-bloc spleen-preserving LDP can be performed by experienced surgeons. This surgery has good short-term and long-term outcome.

Arterial stiffness in type 2 diabetic patients and its related factors

Wang Jundi, Shang Guangyun, He Wei, Wang Hongyu

2017, 38(5):  740-743.  doi:10.3969/j.issn.1006-7795.2017.05.020

Asbtract ( 141 )   PDF (980KB) ( 117 )  

References | Related Articles | Metrics

Objective To explore type 2 diabetes mellitus and arterial stiffness related factors. Methods A total of 582 cases of patients with type 2 diabetes in our hospital from September 2013 to September 2016 as the research objects of the observation group, and selected 384 healthy people in our hospital were enrolled as the control group. Carotid artery ultrasound and pulse wave velocity of the two groups were checked, test results were observed. Results Observation group of patients with systolic blood pressure (SBP), triglyceride (TG), body mass index (BMI), low density lipoprotein-cholesterol (LDL-C), insulin resistance index, C reactive protein (CRP), homocysteine was higher than that of the control group, high density lipoprotein-cholesterol (HDL-C) was lower than that in the control group, there was statistically significant correlation between the two groups (P<0.05). The observation group patients with carotid artery intima-media thickness(IMT), carotid-femoral pulse wave velocity(CF-PWV) higher than that of the control group (P<0.05), the observation group with the plaque detection rate was 47.59%, the control group with the plaque detection rate was 14.22%, the observation group of bilateral carotid artery plaque detection rate was 33.85%, the control group of bilateral carotid artery plaque detection rate was 5.47%, the two groups had significant difference (P<0.05). Analysis of patients with type 2 diabetes mellitus, Logistic regression analysis showed that age,SBP, LDL-C, homeostasis model assessment of insulin resistance (HOMA-IR), and CRP were the major factors affecting the arterial stiffness. Conclusion In patients with type 2 diabetes and hypertension, hyperlipidemia was significantly higher than that in normal group, carotid artery plaque growth more easily, and large artery elasticity decreased obviously, the influence factors of the stiffness are age, SBP, LDL-C, HOMA-IR and CRP. We should strictly control multiple risk factors in the treatment, improve the treatment effect and improve the quality of life.

Clinical analysis of 23 adult cases of invasive salmonellosis

Guo Yiqun, Wang Peng, Yang Chunxia, Gu Li

2017, 38(5):  744-749.  doi:10.3969/j.issn.1006-7795.2017.05.021

Asbtract ( 136 )   PDF (1023KB) ( 122 )  

References | Related Articles | Metrics

Objective To analyze the clinical characteristics of invasive salmonellosis for better clinical diagnosis and management. Methods A retrospective investigation of the clinical manifestation, laboratory test, clinical course and outcome,antimicrobial susceptibility of Salmonella clinical isolates of 23 cases with invasive Salmonellosis were tested between March 2010 and October 2016 in Beijing Chaoyang Hospital. Results Among the 23 invasive cases in this paper,17 patients were male patients,the median age was 62 years;Most of them(78.26%) had the basic disease/conditions. The median age of 12 cases with local infection(including 4 cases of infectious aneurysms)was 67.5 years, and 91.67% had underlying diseases. Most patients presented with high fever(86.96%)and chill (91.3%),the intestinal symptom like diarrhea was infrequent(30.43%).The median count of blood leukocytes was 9.22(2.53,26.33)×10⁹/L,13 cases (56.5%) of white blood cells (WBC)<10×10⁹/L, and eosinophil% less than 0.5% were 78.26%.Nineteen cases were cured or improved, 2 cases transferred to other hospitals,and 2 cases died. The sensitivity of Salmonella to ciprofloxacin and ampicillin were lower (37.5%, 34.78%respectively)(P<0.05).Intermediate to ciprofloxacin accounted for 56.25%.Conclusion The incidence of invasive salmonellosis was associated with advanced age and presence of underlying diseases,which can take the form of a variety of diseases. It was characterized by high fever, chills, rare diarrhea. In the half of the cases, whole blood leukocytes did not increase and acidophilic granulocyte reduced. Clinicians should keep in mind that infected aortic aneurysm is highly possible in patients with atherosclerosis when blood bacteria cultivation result of Salmonella. Antibiotics are chosen to avoid ampicillin and ciprofloxacin, which can be considered for three-generation cephalospore and levofloxacin.

Application of hyperbaric oxygen therapy on moyamoya disease in adult patients

Wang Yong, Li Haidong, Liu Jie, Gao Yali, Lu Minjie, Zhang Xiaoxiao

2017, 38(5):  750-752.  doi:10.3969/j.issn.1006-7795.2017.05.022

Asbtract ( 214 )   PDF (933KB) ( 141 )  

References | Related Articles | Metrics

Objective To investigate the application of hyperbaric oxygen therapy(HBOT) on moyamoya disease in adult patients. Methods The consecutive adult patients with Moyamoya disease admitted in the Department of Hyperbaric oxygen, Fuxing Hospital,Capital Medical University from March 2009 to December 2016 were enrolled. The clinical features, effect after HBOT and cerebrovascular events during HBOT were analyzed. The clinical follow-up duration was 3 months. The National Institutes of Health Stroke Scale (NIHSS) was used to score neurological deficit. The Modified Rankin Scale (mRS) was used to score the prognosis of patients. Results A total of 13 adult patients with moyamoya disease were enrolled into this retrospective study, including 8 cases of ischemic lesion and 5 cases of hemorrhage lesion. In patients with ischemic lesion, 4 patients were bilateral hemispheres involvement and 4 patients were unilateral involvement, 5 patients were treated with revascularization surgery and complicated with cerebral infarction. In patients with hemorrhage lesion, 3 patients showed ventricle hemorrhage, 2 patients showed cerebral hemorrhage broken into ventricles, and all these patients were treated with evacuation of hematoma/or ventriculopuncture. The neurological deficit scores of all patients were poor before hyperbaric oxygen therapy. The number of HBOT ranged from 5 to 40 sessions. The clinical symptoms of 12 patients were improved after HBOT, 1 patient was invalid, 2 patient re-showed cerebral infarction during HBOT, 10 patients had good prognosis at 3 months(mRS ≤ 3). Conclusion HBOT can effectively reduce the postoperative symptoms of moyamoya disease in adult patients and improve prognosis at 90th days, meanwhile HBOT does not significantly increase the occurrence of cerebrovascular events.

Screening results and significance of 9 013 cases of transfusion irregular antibody

Wu Di

2017, 38(5):  753-757.  doi:10.3969/j.issn.1006-7795.2017.05.023

Asbtract ( 139 )   PDF (981KB) ( 105 )  

References | Related Articles | Metrics

Objective To analyze screening results of 9 013 cases of transfusion irregular antibody, and to explore the clinical significance of the irregular antibody screening. Methods Totally 9 013 cases of patients underwent blood transfusion from July 2013 to August 2016 in Beijing Chaoyang Hospital, Capital Medical University were analyzed retrospectively. The irregular antibody screening and identification were applied with micro-column gel technology. The positive rate of irregular antibody, distribution and irregular antibody positive rate in the different departments were analyzed statistically. In addition, the influence of gender and blood transfusion/history of pregnancy on irregular antibody positive rate. Results The irregular antibody positive rate of 9 013 cases of transfusion of blood specimen was 0.63% (57/9 013), in which mainly for the Rh, was accounted for 42.11% (24/57); Second of MNS type, which was accounting for 24.56% (14/57); Other type and proportion were Lewis system (10.53%), Kidd system (8.77%), P (5.26%) and other (8.77%), respectively. The irregular antibody rate in surgery department was 1.06% (14/1 321), significantly higher than other department 0.59% (43/7 692), with significant difference (P<0.05). The irregular antibody positive rate of women transfusion was 0.94% (30/3 187), which was significantly higher than the 0.46% of male patients (27/5 826), and the difference was statistically significant (P<0.05). The irregular antibody positive rate of patients with blood transfusion/pregnancy history was 0.85% (34/3 986), which was significantly higher than that with no history of transfusion blood transfusion/pregnancy[0.46% (23/5 027)] (P<0.05). Conclusion The irregular antibody screening before blood transfusion is of great significance for the improvement of blood transfusion safety, which provides suitable for screening patients with positive blood type. It can reduce clinical immune hemolysis events in order to increase the effectiveness of blood transfusion.

Discoveries of molecular mechanisms controlling the circadian rhythm-the 2017 Nobel Prize in Physiology or Medicine

Li Yuzheng, Zhang Ting, Wang Xiaomin

2017, 38(5):  765-769.  doi:10.3969/j.issn.1006-7795.2017.05.026

Asbtract ( 303 )   PDF (2200KB) ( 257 )  

References | Related Articles | Metrics

In October 2, 2017, the Nobel Assembly at Karolinska Institute has decided to award the 2017 Nobel Prize in Physiology or Medicine jointly to Jeffrey C. Hall, Michael Rosbash and Michael W. Young for their discoveries of molecular mechanisms controlling the circadian rhythm.

Cryo-electron microscopy:capture life in atomic details-the 2017 Nobel Prize in Chemistry

Yang Hui, Li Shentao, Xue Bing

2017, 38(5):  770-776.  doi:10.3969/j.issn.1006-7795.2017.05.027

Asbtract ( 346 )   PDF (8132KB) ( 451 )  

References | Related Articles | Metrics

The Royal Swedish Academy of Sciences has announced that the Nobel Prize in Chemistry 2017 was awarded to Jacques Dubochet, Joachim Frank and Richard Henderson for their developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution.

The 2017 Lasker Awards

Wu Jianhong, Lu Dan, Zhang Ting, Dai Yinmei, Wang Xiaomin

2017, 38(5):  777-781.  doi:10.3969/j.issn.1006-7795.2017.05.028

Asbtract ( 122 )   PDF (5078KB) ( 111 )  

References | Related Articles | Metrics

In September 6, 2017, the list of winners of the year's Lasker Awards were released. The Albert Lasker Basic Medical Research Award honors Michael N. Hall from University of Basel, Switzerland, for his discovery of the nutrient-activated target of rapamycin (TOR) proteins and their central role in the metabolic control of cell growth. The Lasker~DeBakey Clinical Medical Research Award honors Douglas R. Lowy and John T. Schiller from the National Cancer Institute, whose technological advances enabled the development of human papillomavirus (HPV) vaccines, which prevent cervical cancer and other tumors. The 2017 Lasker~Bloomberg Public Service Award honors Planned Parenthood for providing essential health services and reproductive care to millions of women for more than a century.