Overexpression of promyelocytic leukaemia gene correlated with p53 and bcl-2 family in psoriatic peripheral lymphocytes

doi:10.3969/j.issn.1006-7795.2017.05.003

Abstract

Abstract: Objective To investigate the expressions of promyelocytic leukaemia (PML) gene, p53, bcl-2 and bcl-2 associated X protein (bax) in psoriatic peripheral lymphocytes, and evaluate the relationships between PML and those candidate proteins. Methods Twenty-five psoriasis patients and 20 healthy donors were recruited. The peripheral lymphocytes were isolated. The mRNA and protein levels of PML, p53, bcl-2 and bax in peripheral lymphocytes were detected by qRT-PCR and flow cytometry, respectively. Pearson correlation matrix analysis was conducted to evaluate the relationships between PML protein and those candidate proteins.Results The mRNA and protein of PML and p53 were increased, while protein of bcl-2 decreased in peripheral lymphocytes of psoriasis patients as compared to healthy blood donors. The mRNA of bax decreased while protein of bax increased in psoriatic peripheral lymphocytes. The expression of PML protein was correlated with p53, bcl-2 and bax proteins in psoriatic peripheral lymphocytes. Conclusion PML may play a role in the mechanism of psoriasis.

Key words: promyelocytic leukaemia (PML), psoriasis, p53, Bcl-2 family, apoptosis

CLC Number:

R758.63

Peng Shiguang, Li Na, Lan Zulian, Li Li, He Yanling. Overexpression of promyelocytic leukaemia gene correlated with p53 and bcl-2 family in psoriatic peripheral lymphocytes[J]. Journal of Capital Medical University, 2017, 38(5): 645-649.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.05.003

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I5/645

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