Alloantigen immune tolerance and its mechanism in mice immunized with the ultraviolet radiation immature dendritic cells

doi:10.3969/j.issn.1006-7795.2017.03.020

Abstract

Abstract: Objective To induce alloantigen immune tolerance between C3H mice and Balb/c mice by infusing the ultraviolet B(UVB) radiation immature dendritic cells (imDC) and and study the immunological mechanisms of this process.Methods ①The authors induced alloantigen tolerance in C3H mice (H-2k) by intravenous injecting ultraviolet B (UVB) irradiated Balb/c immature dendritic cells (UVB-Balb/c imDC) derived from the cultures of Balb/c bone marrow cells. ②Detection of immune tolerance induced regulatory T cells, interleukin-10 (IL-10) and interferon-γ (IFN-γ) in mice in order to study the tolerance mechanism. Results ①C3H mice immunized with UVB-Balb/c imDC can produce immune tolerance to Balb/c antigens and can not produce anti-Balb/c antibody. ②C3H mice immunized with UVB-Balb/c imDC can not clear the Balb/c spleen cells in vivo. ③The T cells of C3H mice immunized with UVB-Balb/c imDC can secrete more IL-10 and produce more FOX-P3⁺ regulatory T cells than the control group in vitro. Conclusion ①Immunization of C3H mice with UVB-Balb/c imDC allows C3H mice to fully tolerate Balb/c antigen. ②The possible cause of immune tolerance to Balb/c antigens in C3H mice is due to the increased secretion of IL-10 from T cells and the production of more FOX-P3⁺regulatory T cells.

Key words: dendritic cells, apoptosis, regulatory T cells, immune tolerance

CLC Number:

Guo Yixian, Zhang Lanfang, Sun Xuejing, Wan Suigui, Xia Changqing. Alloantigen immune tolerance and its mechanism in mice immunized with the ultraviolet radiation immature dendritic cells[J]. Journal of Capital Medical University, 2017, 38(3): 431-438.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.03.020

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I3/431

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